Identification and functional characterization of phosphorylation sites on GTP cyclohydrolase i

Arteriosclerosis, Thrombosis, and Vascular Biology

Volumn 29, Issue 12, 2009, Pages 2161-2168

  Du, Jianhai ^a,b   Wei, Na ^a,b   Xu, Hao ^a,b   Ge, Ying ^a,b,d   Vásquez Vivar, Jeannette ^c   Guan, Tongju ^a,b   Oldham, Keith T ^a,b   Pritchard, Kirkwood A ^a,b   Shi, Yang ^a,b  

^a Division of Pediatric Surgery   (United States)

^b Children's Research Institution   (United States)

^c MEDICAL COLLEGE OF WISCONSIN   (United States)

^d UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

GTP cyclohydrolase I; Phosphorylation; Tetrahydrobiopterin

Indexed keywords

GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE I; SERINE; TETRAHYDROBIOPTERIN; THREONINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; BIOPTERIN; CELL LINE; CELL NUCLEUS; GTP CYCLOHYDROLASE; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RATS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 73849125957     PISSN: 10795642     EISSN: None     Source Type: Journal    
DOI: 10.1161/ATVBAHA.109.194464     Document Type: Article

References (20)

1. Werner-Felmayer G, Golderer G, Werner ER. Tetrahydrobiopterin biosynthesis, utilization and pharmacological effects. Curr Drug Metab. 2002;3:159-173.
2. Thony B, Auerbach G, Blau N. Tetrahydrobiopterin biosynthesis, regeneration and functions. Biochem J. 2000;347 Pt 1:1-16.
3. Moens AL, Kass DA. Therapeutic potential of tetrahydrobiopterin for treating vascular and cardiac disease. J Cardiovasc Pharmacol. 2007;50: 238-246.
4. Cai S, Alp NJ, McDonald D, Smith I, Kay J, Canevari L, Heales S, Channon KM. GTP cyclohydrolase I gene transfer augments intracellular tetrahydrobiopterin in human endothelial cells: effects on nitric oxide synthase activity, protein levels and dimerisation. Cardiovasc Res. 2002; 55:838-849.
5. Alp NJ, Mussa S, Khoo J, Cai S, Guzik T, Jefferson A, Goh N, Rockett KA, Channon KM. Tetrahydrobiopterin-dependent preservation of nitric oxide-mediated endothelial function in diabetes by targeted transgenic GTP-cyclohydrolase I overexpression. J Clin Invest. 2003;112:725-735.
6. Bendall JK, Alp NJ, Warrick N, Cai S, Adlam D, Rockett K, Yokoyama M, Kawashima S, Channon KM. Stoichiometric relationships between endothelial tetrahydrobiopterin, endothelial NO synthase (eNOS) activity, and eNOS coupling in vivo: insights from transgenic mice with endothe-lial-targeted GTP cyclohydrolase 1 and eNOS overexpression. Circ Res. 2005;97:864-871.
7. Lapize C, Pluss C, Werner ER, Huwiler A, Pfeilschifter J. Protein kinase C phosphorylates and activates GTP cyclohydrolase I in rat renal mes-angial cells. Biochem Biophys Res Commun. 1998;251:802-805.
8. Hesslinger C, Kremmer E, Hultner L, Ueffing M, Ziegler I. Phosphory-lation of GTP cyclohydrolase I and modulation of its activity in rodent mast cells. GTP cyclohydrolase I hyperphosphorylation is coupled to high affinity IgE receptor signaling and involves protein kinase C. J Biol Chem. 1998;273:21616-21622.
9. Widder JD, Chen W, Li L, Dikalov S, Thony B, Hatakeyama K, Harrison DG. Regulation of Tetrahydrobiopterin Biosynthesis by Shear Stress. Circ Res. 2007;101:830-838.
10. Elzaouk L, Laufs S, Heerklotz D, Leimbacher W, Blau N, Resibois A, Thony B. Nuclear localization of tetrahydrobiopterin biosynthetic enzymes. Biochim Biophys Acta. 2004;1670:56-68.
11. Du J, Xu H, Wei N, Wakim B, Halligan B, Pritchard KA Jr, Shi Y. Identification of proteins interacting with GTP cyclohydrolase I. Biochem Biophys Res Commun. 2009;385:143-147.
12. Funderburk CD, Bowling KM, Xu D, Huang Z, O'Donnell JM. A typical N-terminal extensions confer novel regulatory properties on GTP cyclo-hydrolase isoforms in Drosophila melanogaster. J Biol Chem. 2006;281: 33302-33312.
13. Kim S, Choi H, Park ZY. Improved detection of multi-phosphorylated peptides by LC-MS/MS without phosphopeptide enrichment. Mol Cells. 2007;23:340-348.
14. Maita N, Hatakeyama K, Okada K, Hakoshima T. Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein. J Biol Chem. 2004;279:51534-51540.
15. Saunders-Pullman R, Blau N, Hyland K, Zschocke J, Nygaard T, Raymond D, Shanker V, Mohrmann K, Arnold L, Tabbal S, deLeon D, Ford B, Brin M, Chouinard S, Ozelius L, Klein C, Bressman SB. Phe-nylalanine loading as a diagnostic test for DRD: interpreting the utility of the test. Mol Genet Metab. 2004;83:207-212.
16. Chavan B, Gillbro JM, Rokos H, Schallreuter KU. GTP cyclohydrolase feedback regulatory protein controls cofactor 6-tetrahydrobiopterin synthesis in the cytosol and in the nucleus of epidermal keratinocytes and melanocytes. J Invest Dermatol. 2006;126:2481-2489.
17. Jagnandan D, Sessa WC, Fulton D. Intracellular location regulates calcium-calmodulin-dependent activation of organelle-restricted eNOS. Am J Physiol Cell Physiol. 2005;289:C1024-C1033.
18. Gobeil F Jr, Zhu T, Brault S, Geha A, Vazquez-Tello A, Fortier A, Barbaz D, Checchin D, Hou X, Nader M, Bkaily G, Gratton JP, Heveker N, Ribeiro-da-Silva A, Peri K, Bard H, Chorvatova A, D'Orleans-Juste P, Goetzl EJ, Chemtob S. Nitric oxide signaling via nuclearized endothelial nitric-oxide synthase modulates expression of the immediate early genes iNOS and mPGES-1. J Biol Chem. 2006;281:16058-16067.
19. Saini R, Patel S, Saluja R, Sahasrabuddhe AA, Singh MP, Habib S, Bajpai VK, Dikshit M. Nitric oxide synthase localization in the rat neutrophils: immunocytochemical, molecular, and biochemical studies. J Leukoc Biol. 2006;79:519-528.
20. Klinz F-J, Herberg N, Arnhold S, Addicks K, Bloch W. Phospho-eNOS Ser-1176 is associated with the nucleoli and the Golgi complex in C6 rat glioma cells. Neuroscience Letters. 2007;421:224-228.