Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

Theriogenology

Volumn 67, Issue 6, 2007, Pages 1097-1107

  Hoelker, Michael ^a   Mekchay, Supamit ^b   Schneider, Hendrik ^a   Bracket, Benjamin Gaylord ^c   Tesfaye, Dawit ^a   Jennen, Danyel ^a   Tholen, Ernst ^a   Gilles, Markus ^a   Rings, Franka ^a   Griese, Josef ^a   Schellander, Karl ^a  

^a UNIVERSITY OF BONN   (Germany)

^b CHIANG MAI UNIVERSITY   (Thailand)

^c UNIVERSITY OF GEORGIA   (United States)

Author keywords

Cattle; Exogenous DNA; Gene expression; Real time PCR; Sperm mediated

Indexed keywords

DNA; FUGENE; INTERFERON; LIPID; PLACENTA PROTEIN; TROPHOBLAST PROTEIN 1; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; COMPARATIVE STUDY; EMBRYO; EVALUATION; FEMALE; FERTILIZATION IN VITRO; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; MALE; METABOLISM; METHODOLOGY; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATOZOON; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CATTLE; DNA; EMBRYO; FEMALE; FERTILIZATION IN VITRO; GENE EXPRESSION; GENETIC ENGINEERING; INTERFERON TYPE I; LIPIDS; MALE; PLASMIDS; PREGNANCY PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPERMATOZOA; TRANSFECTION;

BOS; BOVINAE; MURINAE;

EID: 33847607522     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2006.12.011     Document Type: Article

References (46)

1. Brackett B.G., Baranska W., Sawicki W., and Koprowski H. Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc Natl Acad Sci USA 68 (1971) 353-357
2. Lavitrano M., Camaioni A., Fazio V.M., Dolci S., Farace M.G., and Spadafora C. Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57 (1989) 717-723
3. Webster NL, Forni M, Bacci ML, Giovannoni R, Razzini R, Fantinati P, et al. Multi-transgenic pigs expressing three fluorescent proteins produced with high efficiency by sperm mediated gene transfer. Mol Reprod Dev 2005;72:68-76.
4. Rieth A., Pothier F., and Sirard M.A. Effect of the target copy number on transgene integration by homologous recombination in bovine embryos. Theriogenology 51 (1999) 425
5. Rieth A., Pothier F., and Sirard M.A. Electroporation of bovine spermatozoa to carry DNA containing highly repetitive sequences into oocytes and detection of homologous recombination events. Mol Reprod Dev 57 (2000) 338-345
6. Niemann H., and Kues W.A. Transgenic livestock: premises and promises. Anim Reprod Sci 60-61 (2000) 277-293
7. Niemann H., Kues W., and Carnwath J.W. Transgenic farm animals: present and future. Rev Sci Tech 24 (2005) 285-298
8. Wolf E., Schernthaner W., Zakhartchenko V., Prelle K., Stojkovic M., and Brem G. Transgenic technology in farm animals-progress and perspectives. Exp Physiol 85 (2000) 615-625
9. Lavitrano M., Busnelli M., Cerrito M.G., Giovannoni R., Manzini S., and Vargiolu A. Sperm-mediated gene transfer. Reprod Fertil Dev 18 (2006) 19-23
10. Patil J.G., and Khoo H.W. Nuclear internalization of foreign DNA by zebrafish spermatozoa and its enhancement by electroporation. J Exp Zool 274 (1996) 121-129
11. Tsai H.J., Lai C.H., and Yang H.S. Sperm as a carrier to introduce an exogenous DNA fragment into the oocyte of Japanese abalone (Haliotis divorsicolor suportexta). Transgenic Res 6 (1997) 85-95
12. Nakanishi A., and Iritani A. Gene transfer in the chicken by sperm-mediated methods. Mol Reprod Dev 36 (1993) 258-261
13. Maione B., Lavitrano M., Spadafora C., and Kiessling A.A. Sperm-mediated gene transfer in mice. Mol Reprod Dev 50 (1998) 406-409
14. Gandolfi F., Lavitrano M., Camaioni A., Spadafora C., Siracusa G., and Lauria A. The use of sperm-mediated gene transfer for the generation of transgenic pics. J Reprod Fertil Abstr Ser 4 (1989) 10
15. Sperandio S., Lulli V., Bacci M.L., Forni M., Maione B., Spadafora C., et al. Sperm-mediated and transfer in bovine and swine. Anim Biotechnol 71 (1996) 59-77
16. Lavitrano M., Forni M., Varzi V., Pucci L., Bacci M.L., Di Stefano C., et al. Sperm-mediated gene transfer: production of pigs transgenic for a human regulator of complement activation. Transplant Proc 29 (1997) 3508-3509
17. Perez A., Soana R., Castro F.O., Leonart R.L., De Armas R., Martinez R., et al. Sperm cells mediated gene transfer in cattle. Biotechnol Aplicada 8 (1991) 95-101
18. Schellander K., Peli J., Schmoll F., and Brem G. Artificial insemination in cattle with DNA-treated sperm. Anim Biotechnol 6 (1995) 41-50
19. Lavitrano M., Forni M., Bacci M.L., Di Stefano C., Varzi V., Wang H., et al. Sperm mediated gene transfer in pig: selection of donor boars and optimization of DNA uptake. Mol Reprod Dev 64 (2003) 284-291
20. Chan A.W., Luetjens C.M., and Schatten G.P. Sperm-mediated gene transfer. Curr Top Dev Biol 50 (2000) 89-102
21. Sciamanna I., Piccoli S., Barberi L., Zaccagnini G., Magnano A.R., Giordano R., et al. DNA dose and sequence dependence in sperm-mediated gene transfer. Mol Reprod Dev 56 (2000) 301-305
22. Anzar M., and Buhr M.M. Spontaneous uptake of exogenous DNA by bull spermatozoa. Theriogenology 65 (2006) 683-690
23. Sasaki S., Kojima Y., Kubota H., Tatsura H., Hayashi Y., and Kohri K. Effects of the gene transfer into sperm mediated by liposomes on sperm motility and fertilization in vitro. Hinyokika Kiyo 46 (2000) 591-595
24. Gagne M.B., Pothier F., and Sirard M.A. Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. Mol Reprod Dev 29 (1991) 6-15
25. Perry D.J., Austin K.J., and Hansen T.R. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol Endocrinol 13 (1999) 1197-1206
26. Bachiller D., Schellander K., Peli J., and Ruther U. Liposome-mediated DNA uptake by sperm cells. Mol Reprod Dev 30 (1991) 194-200
27. Rottmann O., Antes R., Höfer P., and Maierhofer G. Liposome mediated gene-transfer via spermatozoa into avine egg cells. J Anim Breed Genet 109 (1992) 64-70
28. Shen W., Li L., Pan Q., Min L., Dong H., and Deng J. Efficient and simple production of transgenic mice and rabbits using the new DMSO-sperm mediated exogenous DNA transfer method. Mol Reprod Dev 73 (2006) 589-594
29. Lauria A., and Gandolfi F. Recent advances in sperm cell mediated gene transfer. Mol Reprod Dev 56 (1993) 281-284
30. Khoo H.W. Sperm-mediated gene transfer studies on zebrafish in Singapore. Mol Reprod Dev 56 (2000) 278-280
31. Wegner M., Zastrow G., Klavinius A., Schwender S., Muller F., Luksza H., et al. Cis-acting sequences from mouse rDNA promote plasmid DNA amplification and persistence in mouse cells: implication of HMG-I in their function. Nucleic Acids Res 17 (1989) 9909-9932
32. Kulmburg P., Veelken H., Schmoor C., Rosenthal F.M., Mackensen A., Lindemann A., et al. NTS influence on transgene expression from. J Mol Med 75 (1997) 223-229
33. Rottmann O., Höfer P., Sommer B., Wanner G., Görlach A., Grummt F., et al. Liposome-mediated gene transfer via sperm cells. High transfer efficiency and persistence of transgenes by use of liposomes and sperm cells and a murine amplification element. J Anim Breed Genet 113 (1996) 401-411
34. Chan A.W., Luetjens C.M., Dominko T., Ramalho-Santos J., Simerly C.R., Hewitson L., et al. Foreign DNA transmission by ICSI: injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live rhesus monkey births. Mol Hum Reprod 6 (2000) 26-33
35. Macha J., Stursova D., Takac M., Habrova V., and Jonak J. Uptake of plasmid RSV DNA by frog and mouse spermatozoa. Folia Biol (Praha) 43 (1997) 123-127
36. Atkinson P.W., Hines E.R., Beaton S., Matthaei K.I., Reed K.C., and Bradley M.P. Association of exogenous DNA with cattle and insect spermatozoa in vitro. Mol Reprod Dev 29 (1991) 1-5
37. Heid C.A., Stevens J., Livak K.J., and Williams P.M. Real time quantitative PCR. Genome Res 6 (1996) 986-994
38. Parrish J.J., Susko-Parrish J., Winer M.A., and First N.L. Capacitation of bovine sperm by heparin. Biol Reprod 38 (1988) 1171-1180
39. Rosenkrans Jr. C.F., and First N.L. Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro. J Anim Sci 72 (1994) 434-437
40. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25 (2001) 402-408
41. Jang G., Bhuiyan M.M., Jeon H.Y., Ko K.H., Park H.J., Kim M.K., et al. An approach for producing transgenic cloned cows by nuclear transfer of cells transfected with human alpha 1-antitrypsin gene. Theriogenology (2005)
42. Hyun S., Lee G., Kim D., Kim H., Lee S., Nam D., et al. Production of nuclear transfer-derived piglets using porcine fetal fibroblasts transfected with the enhanced green fluorescent protein. Biol Reprod 69 (2003) 1060-1068
43. Hemann C., Gartner E., Weidle U.H., and Grummt F. High-copy expression vector based on amplification-promoting sequences. DNA Cell Biol 13 (1994) 437-445
44. Lavitrano M., French D., Zani M., Frati L., and Spadafora C. The interaction between exogenous DNA and sperm cells. Mol Reprod Dev 31 (1992) 161-169
45. Chan A.W., Kukolj G., Skalka A.M., and Bremel R.D. Timing of DNA integration, transgenic mosaicism, and pronuclear microinjection. Mol Reprod Dev 52 (1999) 406-413
46. Murakami M., Fahrudin M., Varisanga M.D., and Suzuki T. Fluorescence expression by bovine embryos after pronuclear microinjection with the EGFP gene. J Vet Med Sci 61 (1999) 843-847