MS/MS/MS reveals false positive identification of histone serine methylation

Journal of Proteome Research

Volumn 9, Issue 1, 2010, Pages 585-594

  Zhang, Junmei ^a   Chen, Yue ^b   Zhang, Zhihong ^b   Xing, Gang ^a   Wysocka, Joanna ^c   Zhao, Yingming ^b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF CHICAGO   (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

C terminal elimination; Charge dependence; False positive identification; Histone modifications; Loss of C terminal; MS MS MS analysis; Protein methylation; Side chain assisted C terminal rearrangement

Indexed keywords

CARBOXYL GROUP; HISTONE; SERINE; SYNTHETIC PEPTIDE;

ACETYLATION; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CALF (BOVINE); CARBOXY TERMINAL SEQUENCE; CHEMICAL REACTION; COLLISIONALLY ACTIVATED DISSOCIATION; CONFORMATIONAL TRANSITION; DERIVATIZATION; ELUTION; FALSE POSITIVE RESULT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IN VITRO STUDY; ISOTOPE LABELING; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DATABASE; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN METHYLATION; PROTEIN PROCESSING; SEQUENCE ALIGNMENT; TECHNIQUE; THYMUS;

CELL LINE; FALSE POSITIVE REACTIONS; HELA CELLS; HISTONES; HUMANS; ISOTOPE LABELING; METHYLATION; PEPTIDES; SEQUENCE ALIGNMENT; SERINE; TANDEM MASS SPECTROMETRY;

EID: 73649107630     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr900864s     Document Type: Article

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