Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples

Water Science and Technology

Volumn 60, Issue 8, 2009, Pages 2167-2172

  Inomata, A ^a   Kishida, N ^b   Momoda, T ^c   Akiba, M ^b   Izumiyama, S ^d   Yagita, K ^d   Endo, T ^d  

^a TOKYO METROPOLITAN INSTITUTE OF PUBLIC HEALTH   (Japan)

^b NATIONAL INSTITUTE OF PUBLIC HEALTH   (Japan)

^c EIKEN CHEMICAL CO LTD   (Japan)

^d NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Cryptosporidiosis; Cryptosporidium; LAMP; Oocysts; RT LAMP

Indexed keywords

RNA; TRANSCRIPTION;

18S RRNA; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM OOCYSTS; DETECTION LIMITS; MICROSCOPIC OBSERVATIONS; OOCYSTS; REVERSE TRANSCRIPTION; SENSITIVE DETECTION; WATER SAMPLES;

AMPLIFICATION;

RNA 18S; WATER;

AMPLIFICATION; CYST; DETECTION METHOD; MICROSCOPY; PROTOZOAN; RNA; WATER TREATMENT;

CONFERENCE PAPER; CRYPTOSPORIDIUM; NONHUMAN; OOCYST; PARASITE IDENTIFICATION; REVERSE TRANSCRIPTION; WATER CONTAMINATION; WATER SAMPLING; AGAR GEL ELECTROPHORESIS; ANIMAL; ARTICLE; CYTOLOGY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; PARASITOLOGY; STATISTICAL PARAMETERS; TEMPERATURE;

CRYPTOSPORIDIUM;

ANIMALS; CRYPTOSPORIDIUM; ELECTROPHORESIS, AGAR GEL; LIMIT OF DETECTION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OOCYSTS; REVERSE TRANSCRIPTION; TEMPERATURE; WATER;

EID: 73349139563     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2009.599     Document Type: Article

References (21)

1. Carpenter, C., Fayer, R., Trout, J. & Beach, M. J. 1999 Chlorine disinfection of recreational water for Cryptosporidium parvum. Emerg. Infect. Dis. 5(4), 579-584.
2. Cicirello, H. G., Kehl, K. S., Addiss, D. G., Chusid, M. J., Glass, R. I., Davis, J. P. & Havens, P. L. 1997 Cryptosporidiosis in children during a massive waterborne outbreak in Milwaukee, Wisconsin: clinical, laboratory and epidemiologic findings. Epidemiol. Infect. 119(1), 53-60.
3. Corso, P. S., Kramer, M. H., Blair, K. A., Addiss, D. G., Davis, J. P. & Haddix, A. C. 2003 Cost of illness in the 1993 waterborne Cryptosporidium outbreak, Milwaukee, Wisconsin. Emerg. Infect. Dis. 9(4), 426-431.
4. Di Giovanni, G. D., Hashemi, F. H., Shaw, N. J., Abrams, F. A., LeChevallier, M. W. & Abbaszadegan, M. 1999 Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR. Appl. Environ. Microbiol. 65(8), 3427-3432.
5. Fegatella, F., Lim, J., Kjelleberg, S. & Cavicchioli, R. 1998 Implications of rRNA operon copy number and ribosome content in the marine oligotrophic ultramicrobacterium Sphingomonas sp. strain RB2256. Appl. Environ. Microbiol. 64(11), 4433-4438.
6. Hirata, T. & Hashimoto, A. 2006 Genotyping of single Cryptosporidium oocysts isolated from sewage and river water. Water Sci. Technol. 54(3), 197-202.
7. Lodish, H., Berk, A., Zipursky, L. S., Matsudaira, P., Baltimore, D. & Darnell, J. 2000 Processing of rRNA and tRNA. In: Molecular Cell Biology, 4th edition, W. H. Freeman and Company, New York. http://www.ncbi.nlm.nih.gov/ books/bv.fcgi?rid=mcb.section.2975 (accessed 10 June 2009).
8. MacKenzie, W. R., Schell, W. L., Blair, K. A., Addiss, D. G., Peterson, D. E., Hoxie, N. J., Kazmierczak, J. J. & Davis, J. P. 1995 Massive outbreak of waterborne Cryptosporidium infection in Milwaukee, Wisconsin: recurrence of illness and risk of secondary transmission. Clin. Infect. Dis. 21(1), 57-62.
9. Masago, Y., Oguma, K., Katayama, H. & Ohgaki, S. 2006 Quantification and genotyping of Cryptosporidium spp. in river water by quenching probe PCR and denaturing gradient gel electrophoresis. Water Sci. Technol. 54(3), 119-126.
10. Methods for the Detection of Cryptosporidium, Giardia and Indicator Microorganisms in Water Supply Systems 2007 Ministry of Health, Labour and Welfare, Tokyo, Japan.
11. Momoda, T., Kojima, T., Ikedo, M., Izumiyama, S. & Endo, T. 2009 Sensitive and rapid detection of Cryptosporidium and Giardia by loop-mediated isothermal amplification. J. Jpn Soc. Water Environ. 32(6), 321-324.
12. Monis, P. T. & Saint, C. P. 2001 Development of a nested-PCR assay for the detection of Cryptosporidium parvum in finished water. Water Res. 35(7), 1641-1648.
13. Nichols, G. 2008 Epidemiology. In: Fayer, R. & Xiao, L. (eds) Cryptosporidium and Cryptosporidiosis, 2nd edition. IWA Publishing, London, UK, pp. 79-118.
14. Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N. & Hase, T. 2000 Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28(12), e63.
15. O'Donoghue, P. J. 1995 Cryptosporidium and cryptosporidiosis in man and animals. Int. J. Parasitol. 25(2), 139-195.
16. Ohtsuka, K., Yanagawa, K., Takatori, K. & Hara-Kudo, Y. 2005 Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates. Appl. Environ. Microbiol. 71(11), 6730-6735.
17. Paul, J. H., Jeffrey, W. H., David, A. W., DeFlaun, M. F. & Cazares, L. H. 1989 Turnover of extracellular DNA in eutrophic and oligotrophic freshwater environments of Southwest Florida. Appl. Environ. Microbiol. 55(7), 1823-1828.
18. Peeters, J. E., Mazas, E. A., Masschelein, W. J., Villacorta Martiez de Maturana, I. & Debacker, E. 1989 Effect of disinfection of drinking water with ozone or chlorine dioxide on survival of Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 55(6), 1519-1522.
19. Ramirez, N. E. & Sreevatsan, S. 2006 Development of a sensitive detection system for Cryptosporidium in environmental samples. Vet. Parasitol. 136(3-4), 201-213.
20. Tani, H., Teramura, T., Adachi, K., Tsuneda, S., Kurata, S., Nakamura, K., Kanagawa, T. & Noda, N. 2007 Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop-mediated isothermal amplification. Anal. Chem. 79(15), 5608-5613.
21. Yagita, K., Izumiyama, S., Tachibana, H., Masuda, G., Iseki, M., Furuya, K., Kameoka, Y., Kuroki, T., Itagaki, T. & Endo, T. 2001 Molecular characterization of Cryptosporidium isolates obtained from human and bovine infections in Japan. Parasitol. Res. 87(11), 950-955.