Genotyping of single Cryptosporidium oocysts isolated from sewage and river water

Water Science and Technology

Volumn 54, Issue 3, 2006, Pages 197-202

  Hirata, T ^a   Hashimoto, Atsushi ^a,b  

^a AZABU UNIVERSITY   (Japan)

^b ANAN NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

18S rRNA; Cryptosporidium; Environmental isolates; Genotyping; Semi nested PCR; Single oocyst

Indexed keywords

CRYPTOSPORIDIUM; GENOTYPING; OOCYST;

CELLS; ENVIRONMENTAL IMPACT; GENETIC ENGINEERING; RIVER POLLUTION; RNA; SEWAGE;

WATER ANALYSIS;

FLUORESCEIN ISOTHIOCYANATE; RIVER WATER; RNA 18S;

CELLS; ENVIRONMENTAL IMPACT; GENETIC ENGINEERING; RIVER POLLUTION; RNA; SEWAGE; WATER ANALYSIS;

CELL ORGANELLE; GENOTYPE; POLYMERASE CHAIN REACTION; PROTOZOAN; RIVER WATER; RNA; SEWAGE;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; DNA SEQUENCE; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYST; POLYMERASE CHAIN REACTION; SEWAGE; WATER SAMPLING;

ANIMALS; BASE SEQUENCE; CRYPTOSPORIDIUM; DNA PRIMERS; GENOTYPE; OOCYSTS; RIVERS; SEWAGE; WATER;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 33749154058     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2006.469     Document Type: Article

References (9)

1. Hashimoto, A., et al. (2002). Prevalence of Cryptosporidium oocysts and Giardia cysts in the drinking water supply in Japan. Water Res., 36, 519-526.
2. Jellison, K.L., et al. (2002). Sources and species of Cryptosporidium oocysts in the Wachusett reservoir watershed. Appl. Environ. Microbiol., 68, 569-575.
3. Johnson, D.W., et al. (1995). Development of PCR protocol for sensitive detection of Cryptosporidium oocysts in water sample. Appl. Environ. Microbiol., 61, 3849-3855.
4. Morgan, U.M., et al. (1997). Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis. J. Parasitol., 83, 825-830.
5. Pieniazek, N.J., et al. (1999). New Cryptosporidium genotypes in HIV-infected persons. Emer. Infect. Dis., 5, 444-449.
6. Sturbaum, G.D., et al. (2002). Immunomagnetic separation (IMS)-fluorescent antibody detection and IMS-PCR detection of seeded Cryptosporidium parvum oocysts in natural waters and their limitations. Appl. Environ. Microbiol., 68, 2991-2996.
7. USEPA (1999) Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. USEPA.
8. Ward, P.I., et al. (2003). Detection of eight Cryptosporiditan genotypes in surface and waste water in Europe. Parasitol., 124, 359-368.
9. Xiao, L., et al. (2000). Identification of species and source of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl. Environ. Microbiol., 66, 5492-5498.