Development and in-house validation of the event-specific qualitative and quantitative PCR detection methods for genetically modified cotton MON15985

Journal of the Science of Food and Agriculture

Volumn 90, Issue 3, 2010, Pages 402-408

  Jiang, Lingxi ^a   Yang, Litao ^a   Rao, Jun ^a   Guo, Jinchao ^a   Wang, Shu ^a   Liu, Jia ^a   Leeb, Seonghun ^b   Zhanga, Dabing ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b Ministry of Agriculture and Forestry Korea   (South Korea)

Author keywords

Event specific; Genetically modified cotton; In house validation; Mon15985

Indexed keywords

PLANT DNA; PRIMER DNA;

ARTICLE; COTTON; GENETICS; HAPLOIDY; METHODOLOGY; NUCLEOTIDE SEQUENCE; PLANT GENOME; POLYMERASE CHAIN REACTION; STATISTICAL PARAMETERS; TRANSGENIC PLANT; VALIDATION STUDY;

BASE SEQUENCE; DNA PRIMERS; DNA, PLANT; GENOME, PLANT; GOSSYPIUM; HAPLOIDY; LIMIT OF DETECTION; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION;

GOSSYPIUM HIRSUTUM;

EID: 73349107814     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.3829     Document Type: Article

References (20)

1. James C, Executive summary of global status of commercialized biotech/GM crops ISAAA Briefs 39:(2008).
2. European Commission Regulation (EC)No. 1829/2003 and 1830/2003. Off J Eur Commun L268:1-28 (2003).
3. Notification 2000-31, Ministry of Agriculture and Forestry of Korea, Seoul, Korea (2000).
4. Notification1775, Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan, Tokyo (2000).
5. Order 10, Ministry of Agriculture of the People's Republic of China, Beijing (2002).
6. Miraglia M, Berdal KG, Brera C, Corbisier P, Holst-Jensen A, Kok EJ, et al, Detection and traceability of genetically modified organisms in the food production chain. Food Chem Toxicol 42:1157-1180 (2004).
7. Holst-Jensen A, Røning SB, Løseth A and Berdal KG, PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal Chem 375:985-993 (2003).
8. China Cotton Network [Online]. China's GM cotton area increased. Available: http://www.cncotton.com/gnmh/scdt/xh/qy/200803/t20080305 152865.html [2 November 2009].
9. Agbios [Online]. GM Database Available: http://www.agbios.com/dbase.php?action=ShowProd&data=15985 [2 November 2009].
10. Lee SH, Kee JK and Yi BY, Detection methods for biotech cotton MON15985 and MON88913 by PCR. J Agric Food Chem 55: 3351-3357 (2007).
11. Yang LT, Pan AH, Zhang KW, Guo JC, Yin CS, Chen JX, et al, Identification and quantification of three genetically modified insect resistant cotton lines using conventional and Taq Man real-time polymerase chain reaction methods. J Agric Food Chem 53:6222-6229 (2005).
12. Yang LT, Pan AH, Zhang KW, Yin CS, Qian BJ, Chen JX, et al, Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton MON1445 and MON531. Transgen Res 14:817-831 (2005).
13. Event-specific method for the quantification of cotton line MON 15985 using real-time PCR. [Online]. Available: http://gmo-crl.jrc.ec.europa.eu/summaries/MON15985validated Method.pdf [2 November 2009].
14. Arumuganathan K and Earle ED, Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208-218 (1991).
15. 15 GMO detection method database [Online]. Available: http://gmdd.shgmo.org/ [2 November 2009].
16. Dong W, Yang LT, Shen KL, Banghyun K, Gijs AK, Hans JPM, et al, GMDD: a database of GMO detection methods. BMC Bioinform 9:260 (2008).
17. Yang LT, Chen JX, Cheng H, Liu YH, Jia SR, Pan LW, et al, Validation of a cotton-specific gene, Sad I, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons. Plant Cell Rep 24:237-245 (2005).
18. Berdal KG and Holst-Jensen A, Roundup Ready soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur Food Res Technol 213:432-438 (2001).
19. Review of GMO Detection and Quantification Techniques. European Commission, Joint Research Centre, Brussels (2001).
20. Definition of minimum performance requirements for analytical methods of GMO testing - ENGL. [Online]. Available: http://gmo-crl.jrc.ec.europa.eu/doc/method%20requirements.pdf.[16November 2009].