Purification and on-column activation of a recombinant histidine-tagged pro-transglutaminase after soluble expression in Escherichia coli

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 11, 2009, Pages 2531-2534

  Yang, Hui Lin ^a   Pan, Li ^a   Lin, Ying ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Escherichia coli; High density culture; Microbial transglutaminase; On column activation; Soluble recombinant expression

Indexed keywords

FUSION PROTEINS; HIGH-DENSITY CULTURES; MICROBIAL TRANSGLUTAMINASE; ON-COLUMN ACTIVATION; PROTEIN CROSSLINKING; RECOMBINANT EXPRESSION; SDS-PAGE; SOLUBLE EXPRESSION; STREPTOMYCES MOBARAENSIS; TRANSGLUTAMINASES;

DIFFUSERS (OPTICAL); GAS GENERATORS; PROTEINS; THICKNESS GAGES;

ESCHERICHIA COLI;

BOVINE SERUM ALBUMIN; ENZYME PRECURSOR; HISTIDINE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT PROTEIN; TRYPSIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY; STREPTOMYCES;

ANIMALS; CATTLE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME PRECURSORS; ESCHERICHIA COLI; GENE EXPRESSION; HISTIDINE; RECOMBINANT PROTEINS; SERUM ALBUMIN, BOVINE; SOLUBILITY; STREPTOMYCES; TRANSGLUTAMINASES; TRYPSIN;

ESCHERICHIA COLI; STREPTOMYCES; STREPTOMYCES MOBARAENSIS;

EID: 72949124423     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90422     Document Type: Article

References (25)

1. Folk JE, Ann. Rev. Biochem., 49, 517-531 (1980).
2. Chung SI, Ann. NY Acad. Sci., 202, 240-255 (1972).
3. Folk JE and Cole PW, J. Biol. Chem. 241, 5518-5525 (1966.
4. Yasueda H, Nakanishi K, Kumazawa K, Nagase K, Motoki M, and Matsui H, Eur. J. Biochem., 232, 411-419 (1995).
5. Facchiano A and Facchiano F, Amino Acids, 36, 599-614 (2009).
6. Beninati S and Piacentini M, Amino Acids, 26, 367-372 (2004).
7. Ando H, Adachi M, Umeda K, Matsuura A, Nonaka M, Uchio R, Tanaka H, and Motoki M, Agric. Biol. Chem., 53, 2613-2617 (1989).
8. Duran R, Junqua M, Schmitter JM, Gancet C, and Goulas P, Biochimie, 80, 313-319 (1998).
9. Kobayashi K, Kumazawa Y, Miwa K, and Yamanaka S, FEMS Microbiol. Lett., 144, 157-160 (1996).
10. Zilhao R, Isticato R, Martins LO, Steil L, Volker U, Ricca E, Moran CPJr, and Henriques AO, J. Bacteriol., 187, 7753-7764 (2005).
11. Yokoyama K, Nio N, and Kikuchi Y, Appl. Microbiol. Biotechnol., 64, 447-454 (2004).
12. Takehana S, Washizu K, Ando K, Koikeda S, Takeuchi K, Matsui H, Motoki M, and Takagi H, Biosci. Biotechnol. Biochem., 58, 88-92 (1994).
13. Kawai M, Takehana S, and Takagi H, Biosci. Biotechnol. Biochem., 61, 830-835 (1997).
14. Yokoyama KI, Nakamura N, Seguro K, and Kubota K, Biosci. Biotechnol. Biochem., 64, 1263-1270 (2000).
15. Liu XQ, Yang XQ, Xie FH, Song LY, Zhang GQ, and Qian SJ, Protein Expr. Purif., 51, 179-186 (2007).
16. Washizu K, Ando K, Koikeda S, Hirose S, Matsuura A, Takagi H, Motoki M, and Takeuchi K, Biosci. Biotechnol. Biochem., 58, 82-87 (1994).
17. Marx CK, Hertel TC, and Pietzsch M, Enzyme Microb. Technol., 40, 1543-1550 (2007).
18. Yu YJ, Wu SC, Chan HH, Chen YC, Chen ZY, and Yang MT, Appl. Microbiol. Biotechnol., 81, 523-532 (2008).
19. Date M, Yokoyama K, Umezawa Y, Matsui H, and Kikuchi Y, J. Biotechnol., 110, 219-226 (2004).
20. Kikuchi Y, Date M, Yokoyama K, Umezawa Y, and Matsui H, Appl. Environ. Microbiol., 69, 358-366 (2003).
21. Zotzel J, Keller P, and Fuchsbauer HL, Eur. J. Biochem., 270, 3214-3222 (2003).
22. Zotzel J, Pasternack R, Pelzer C, Ziegert D, Mainusch M, and Fuchsbauer HL, Eur. J. Biochem., 270, 4149-4155 (2003).
23. Marx CK, Hertel TC, and Pietzsch M, Enzyme Microb. Technol., 42, 568-575 (2008).
24. Neu HC and Heppel LA, J. Biol. Chem., 240, 3685-3692 (1965).
25. Pasternack R, Dorsch S, Otterbach JT, Robenek IR, Wolf S, and Fuchsbauer HL, Eur. J. Biochem., 257, 570-576 (1998).