5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line

Biochemical and Biophysical Research Communications

Volumn 391, Issue 1, 2010, Pages 305-309

  Lefebvre, Bruno ^a,b   Belaich, Sandrine ^a,b   Longue, Justine ^a,b   Vandewalle, Brigitte ^a,b   Oberholzer, Jose ^d   Gmyr, Valery ^a,b   Pattou, François ^a,b,c   Kerr conte, Julie ^a,b  

^a UNIV LILLE   (France)

^b INSERM   (France)

^c LILLE UNIVERSITY HOSPITAL   (France)

^d UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

5 Azadeoxycytidine; Endocrine differentiation; Pancreatic human ductal epithelial cells; Reprogramming; Transdifferentiation

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; BETACELLULIN; GLUCAGON; NEUROGENIN 3; SOMATOSTATIN; AZACITIDINE; BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; DNA METHYLTRANSFERASE; DRUG DERIVATIVE; NERVE PROTEIN; NEUROG3 PROTEIN, MOUSE; TRANSCRIPTION FACTOR;

ARTICLE; CELL DIFFERENTIATION; CELL LINE; CELL MATURATION; EMBRYO; EMBRYO DEVELOPMENT; ENDOCRINE CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; PANC 1 HUMAN DUCTAL CELL LINE; PANCREAS; PANCREAS ISLET CELL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; BIOSYNTHESIS; CELL TRANSDIFFERENTIATION; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; METABOLISM; PANCREAS DUCT; PANCREAS ISLET;

AZACITIDINE; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CELL DIFFERENTIATION; CELL LINE; CELL TRANSDIFFERENTIATION; DNA MODIFICATION METHYLASES; HUMANS; ISLETS OF LANGERHANS; NERVE TISSUE PROTEINS; PANCREATIC DUCTS; TRANSCRIPTION FACTORS;

EID: 72949124420     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.11.054     Document Type: Article

References (43)

1. Mineo D., Pileggi A., Alejandro R., and Ricordi C. Point: steady progress and current challenges in clinical islet transplantation. Diabetes Care 32 (2009) 1563-1569
2. Khan M.H., and Harlan D.M. Counterpoint: clinical islet transplantation: not ready for prime time. Diabetes Care 32 (2009) 1570-1574
3. Inada A., Bonner-Weir S., and Toschi E. How can we get more beta cells?. Curr. Diab. Rep. 6 (2006) 96-101
4. Nir T., and Dor Y. How to make pancreatic beta cells - prospects for cell therapy in diabetes. Curr. Opin. Biotechnol. 16 (2005) 524-529
5. Dor Y., Brown J., Martinez O.I., and Melton D.A. Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 429 (2004) 41-46
6. Lechner A., and Habener J.F. Stem/progenitor cells derived from adult tissues: potential for the treatment of diabetes mellitus. Am. J. Physiol. Endocrinol. Metab. 284 (2003) E259-E266
7. Parnaud G., Bosco D., Berney T., Pattou F., Kerr-Conte J., Donath M.Y., Bruun C., Mandrup-Poulsen T., Billestrup N., and Halban P.A. Proliferation of sorted human and rat beta cells. Diabetologia 51 (2008) 91-100
8. Xu X., D'Hoker J., Stange G., Bonne S., De L.N., Xiao X., Van de C.M., Mellitzer G., Ling Z., Pipeleers D., Bouwens L., Scharfmann R., Gradwohl G., and Heimberg H. Beta cells can be generated from endogenous progenitors in injured adult mouse pancreas. Cell 132 (2008) 197-207
9. Gu G., Brown J.R., and Melton D.A. Direct lineage tracing reveals the ontogeny of pancreatic cell fates during mouse embryogenesis. Mech. Dev. 120 (2003) 35-43
10. Murtaugh L.C., and Melton D.A. Genes, signals, and lineages in pancreas development. Annu. Rev. Cell Dev. Biol. 19 (2003) 71-89
11. Schonhoff S.E., Giel-Moloney M., and Leiter A.B. Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types. Dev. Biol. 270 (2004) 443-454
12. Gu G., Dubauskaite J., and Melton D.A. Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development 129 (2002) 2447-2457
13. Gradwohl G., Dierich A., LeMeur M., and Guillemot F. Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas. Proc. Natl. Acad. Sci. USA 97 (2000) 1607-1611
14. Wang S., Hecksher-Sorensen J., Xu Y., Zhao A., Dor Y., Rosenberg L., Serup P., and Gu G. Myt1 and Ngn3 form a feed-forward expression loop to promote endocrine islet cell differentiation. Dev. Biol. 317 (2008) 531-540
15. Heremans Y., Van de C.M., in't V.P., Gradwohl G., Serup P., Madsen O., Pipeleers D., and Heimberg H. Recapitulation of embryonic neuroendocrine differentiation in adult human pancreatic duct cells expressing neurogenin 3. J. Cell Biol. 159 (2002) 303-312
16. Harb G., Heremans Y., Heimberg H., and Korbutt G.S. Ectopic expression of neurogenin 3 in neonatal pig pancreatic precursor cells induces (trans)differentiation to functional alpha cells. Diabetologia 49 (2006) 1855-1863
17. Haumaitre C., Lenoir O., and Scharfmann R. Histone deacetylase inhibitors modify pancreatic cell fate determination and amplify endocrine progenitors. Mol. Cell. Biol. 28 (2008) 6373-6383
18. Hendy G.N., Bevan S., Mattei M.G., and Mouland A.J. Chromogranin A. Clin. Invest. Med. 18 (1995) 47-65
19. Jensen J. Gene regulatory factors in pancreatic development. Dev. Dyn. 229 (2004) 176-200
20. Park M.K., Han C., Lee K.H., Hong S.H., Kim H.S., Lee Y.J., Jeong I.K., Noh J.H., Yang T.Y., Lee M.S., Kim K.W., and Lee M.K. Effects of activin A on pancreatic ductal cells in streptozotocin-induced diabetic rats. Transplantation 83 (2007) 925-930
21. Li L., Yi Z., Seno M., and Kojima I. Activin A and betacellulin: effect on regeneration of pancreatic beta-cells in neonatal streptozotocin-treated rats. Diabetes 53 (2004) 608-615
22. Frandsen U., Porneki A.D., Floridon C., Abdallah B.M., and Kassem M. Activin B mediated induction of Pdx1 in human embryonic stem cell derived embryoid bodies. Biochem. Biophys. Res. Commun. 362 (2007) 568-574
23. Zhan X.R., Li X.Y., Liu X.M., Zhou J.H., Yang Y.L., Yi R., Zhang J., and Yang B.F. Generation of insulin-secreting cells from adult rat pancreatic ductal epithelial cells induced by hepatocyte growth factor and betacellulin-delta4. Biochem. Biophys. Res. Commun. 382 (2009) 375-380
24. Yoshikumi Y., Ohno H., Suzuki J., Isshiki M., Morishita Y., Ohnishi H., Yasuda H., Omata M., Fujita T., and Mashima H. Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets. Endocr. J. 55 (2008) 757-765
25. Vaca P., Berna G., Araujo R., Carneiro E.M., Bedoya F.J., Soria B., and Martin F. Nicotinamide induces differentiation of embryonic stem cells into insulin-secreting cells. Exp. Cell Res. 314 (2008) 969-974
26. Zhao M., Amiel S.A., Christie M.R., Rela M., Heaton N., and Huang G.C. Insulin-producing cells derived from human pancreatic non-endocrine cell cultures reverse streptozotocin-induced hyperglycaemia in mice. Diabetologia 48 (2005) 2051-2061
27. Vaca P., Martin F., Vegara-Meseguer J.M., Rovira J.M., Berna G., and Soria B. Induction of differentiation of embryonic stem cells into insulin-secreting cells by fetal soluble factors. Stem cells 24 (2006) 258-265
28. Brolen G.K., Heins N., Edsbagge J., and Semb H. Signals from the embryonic mouse pancreas induce differentiation of human embryonic stem cells into insulin-producing beta-cell-like cells. Diabetes 54 (2005) 2867-2874
29. Hui H., Wright C., and Perfetti R. Glucagon-like peptide 1 induces differentiation of islet duodenal homeobox-1-positive pancreatic ductal cells into insulin-secreting cells. Diabetes 50 (2001) 785-796
30. Hardikar A.A., Marcus-Samuels B., Geras-Raaka E., Raaka B.M., and Gershengorn M.C. Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates. Proc. Natl. Acad. Sci. USA 100 (2003) 7117-7122
31. Gasa R., Mrejen C., Leachman N., Otten M., Barnes M., Wang J., Chakrabarti S., Mirmira R., and German M. Proendocrine genes coordinate the pancreatic islet differentiation program in vitro. Proc. Natl. Acad. Sci. USA 101 (2004) 13245-13250
32. Schwitzgebel V.M., Scheel D.W., Conners J.R., Kalamaras J., Lee J.E., Anderson D.J., Sussel L., Johnson J.D., and German M.S. Expression of neurogenin3 reveals an islet cell precursor population in the pancreas. Development 127 (2000) 3533-3542
33. Apelqvist A., Li H., Sommer L., Beatus P., Anderson D.J., Honjo T., Hrabe de A.M., Lendahl U., and Edlund H. Notch signalling controls pancreatic cell differentiation. Nature 400 (1999) 877-881
34. Grapin-Botton A., Majithia A.R., and Melton D.A. Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Genes Dev. 15 (2001) 444-454
35. Hussain M.A., Miller C.P., and Habener J.F. Brn-4 transcription factor expression targeted to the early developing mouse pancreas induces ectopic glucagon gene expression in insulin-producing beta cells. J. Biol. Chem. 277 (2002) 16028-16032
36. Sosa-Pineda B., Chowdhury K., Torres M., Oliver G., and Gruss P. The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas. Nature 386 (1997) 399-402
37. Dohrmann C., Gruss P., and Lemaire L. Pax genes and the differentiation of hormone-producing endocrine cells in the pancreas. Mech. Dev. 92 (2000) 47-54
38. Kim S.K., and Hebrok M. Intercellular signals regulating pancreas development and function. Genes Dev. 15 (2001) 111-127
39. Kim S.K., Hebrok M., and Melton D.A. Notochord to endoderm signaling is required for pancreas development. Development 124 (1997) 4243-4252
40. Otonkoski T., Beattie G.M., Mally M.I., Ricordi C., and Hayek A. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J. Clin. Invest. 92 (1993) 1459-1466
41. Bhushan A., Itoh N., Kato S., Thiery J.P., Czernichow P., Bellusci S., and Scharfmann R. Fgf10 is essential for maintaining the proliferative capacity of epithelial progenitor cells during early pancreatic organogenesis. Development 128 (2001) 5109-5117
42. Norgaard G.A., Jensen J.N., and Jensen J. FGF10 signaling maintains the pancreatic progenitor cell state revealing a novel role of Notch in organ development. Dev. Biol. 264 (2003) 323-338
43. Miralles F., Lamotte L., Couton D., and Joshi R.L. Interplay between FGF10 and Notch signalling is required for the self-renewal of pancreatic progenitors. Int. J. Dev. Biol. 50 (2006) 17-26