Surface morphology and mechanical properties of fibroblasts from scleroderma patients

Journal of Cellular and Molecular Medicine

Volumn 13, Issue 8 B, 2009, Pages 1644-1652

  Reich, Adam ^a,b,c   Meurer, Michael ^b   Eckes, Beate ^d   Friedrichs, Jens ^c   Muller, Daniel J ^c  

^a WROCLAW MEDICAL UNIVERSITY   (Poland)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^d UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Atomic force microscopy; Cytoskeleton; Extracellular matrix; Fibroblast; Morphea; Systemic sclerosis

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; ATOMIC FORCE MICROSCOPY; CASE CONTROL STUDY; CHILD; EXTRACELLULAR MATRIX; FEMALE; FIBROBLAST; HUMAN; MALE; METABOLISM; PATHOLOGY; SYSTEMIC SCLEROSIS;

ADOLESCENT; ADULT; AGED; CASE-CONTROL STUDIES; CHILD; EXTRACELLULAR MATRIX; FEMALE; FIBROBLASTS; HUMANS; MALE; MICROSCOPY, ATOMIC FORCE; SCLERODERMA, SYSTEMIC; YOUNG ADULT;

EID: 72949104604     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2008.00401.x     Document Type: Article

References (36)

1. Denton CP, Abraham DJ. Transgenic analysis of scleroderma: understanding key pathogenic events in vivo. Autoimmun. Rev. 2004 3 : 285 293.
2. Liu B, Connolly M K. The pathogenesis of cutaneous fibrosis. Semin Cutan Med Surg. 1998 17 : 3 11.
3. Varga J, Abraham D. Systemic sclerosis: a prototypic multisystem fibrotic disorder. J Clin Invest 2007 117 : 557 567.
4. Sandorfi N, Jimenez SA. Scleroderma fibroblast survival in action. J Invest Dermatol. 2005 124 : VIII XI.
5. Wallis DD, Tan FK, Kielty CM, et al. Abnormalities in fibrillin-1 containing microfibrils in dermal fibroblasts cultures from patients with systemic sclerosis (scleroderma). Arthritis Rheum. 2001 44 : 1855 1864.
6. Leask A, Denton CP, Abraham DJ. Insights into the molecular mechanism of chronic fibrosis: the role of connective tissue growth factor in scleroderma. J Invest Dermatol. 2004 122 : 1 6.
7. Baroni SS, Santillo M, Bevilacqua F, et al. Stimulatory autoantibodies to the PDGF receptor in systemic sclerosis. New Engl J Med. 2006 354 : 2667 2676.
8. Eckes B, Kessler D, Aumailley M, et al. Interactions of fibroblasts with the extracellular matrix: implications for the understanding of fibrosis. Springer Semin Immunopatho. 2000 21 : 415 429.
9. Zaman MH, Trapani LM, Sieminski AL, et al. Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis. Proc Natl Acad Sci USA. 2006 103 : 10889 10894.
10. Grinnell F, Ho C-H, Tamariz E, et al. Dendritic fibroblasts in three-dimensional collagen matrices. Mol Biol Cell. 2003 14 : 384 395. (Pubitemid 36237003)
11. Kessler D, Dethlefsen S, Haase I, et al. Fibroblasts in mechanically stressed collagen lattices assume a "synthetic" phenotype. J Biol Chem. 2001 276 : 36575 36585.
12. Eckes B, Zweers MC, Zhang Z G, et al. Mechanical tension and integrin α2β1 regulate fibroblast functions. J Invest Dermatol Symp Proc. 2006 11 : 66 72.
13. Binnig G, Quate CF, Gerber C. Atomic force microscopy. Phys Rev Lett. 1986 56 : 930 933.
14. Poole K, Muller DJ. Flexible, actin-based ridges colocalise with the β1 integrin on the surface of melanoma cells. Br J Cancer. 2005 92 : 1499 1505.
15. Al-Hassan E, Heinz WF, Antonik MD, et al. Relative microelastic mapping of living cells by atomic force microscopy. Biophys J. 1998 74 : 1564 1578.
16. Reich A, Lehmann B, Meurer M, et al. Structural alterations provoked by narrow-band ultraviolet B in immortalized keratinocytes: assessment by atomic force microscopy. Exp Dermatol. 2007 16 : 1007 1015.
17. Engel A, Schoenenberger C-A, Muller DJ. High resolution imaging of native biological sample surfaces using scanning probe microscopy. Curr Opin Struct Biol. 1997 7 : 279 284.
18. Gorzelanny C, Goerge T, Schnaeker E-M, et al. Atomic force microscopy as an innovative tool for nanoanalysis of native stratum corneum. Exp Dermatol. 2006 15 : 387 391.
19. Braet F, Seynaeve C, De Zanger R, et al. Imaging surface and submembranous structures with the atomic force microscope: a study on living cancer cells, fibroblasts and macrophages. J Microsc. 1998 190 : 328 338.
20. Haga H, Nagayama M, Kawabata K, et al. Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM. J Electron Microsc. 2000 49 : 473 481.
21. Rotsch C, Radmacher M. Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study. Biophys J. 2000 78 : 520 535.
22. Kuznetsov YG, Datta S, Kothari NH, et al. Atomic force microscopy investigation of fibroblasts infected with wild-type and mutant murine leukaemia virus (MuLV). Biophys J. 2002 83 : 3665 3674.
23. Sinniah K, Paauw J, Ubels J. Investigating live and fixed epithelial and fibroblast cells by atomic force microscopy. Curr Eye Res. 2002 25 : 61 8.
24. Mahaffy RE, Park S, Gerde E, et al. Quantitative analysis of the viscoelastic properties of thin regions of fibroblasts using atomic force microscopy. Biophys J. 2004 86 : 1777 1793.
25. Franz C, Muller D. Analyzing focal adhesion structure by atomic force microscopy. J Cell Sci. 2005 118 : 5315 5323.
26. Hutter JL, Chen J, Wan WK, et al. Atomic force microscopy investigation of the dependence of cellular elastic moduli on glutaraldehyde fixation. J Microsc. 2005 219 : 61 8.
27. Murphy MF, Lalor MJ, Manning FC, et al. Comparative study of the conditions required to image live human epithelial and fibroblast cells using atomic force microscopy. Micros Res Tech. 2006 69 : 757 765.
28. Kidoaki S, Matsuda T. Shape-engineered fibroblasts: cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy. J Biomed Mater Res A. 2007 81 : 803 810.
29. Hoh JH, Schoenenberger CA. Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. J Cell Sci. 1994 107 : 1105 1114.
30. Nho RS, Xia H, Kahm J, et al. Role of integrin-linked kinase in regulating phosphorylation of Akt and fibroblast survival in type I collagen matrices through a β1 integrin viability signaling pathway. J Biol Chem. 2005 280 : 26630 26639.
31. Kidoaki S, Matsuda T, Yoshikawa K. Relationship between apical membrane elasticity and stress fiber organization in fibroblasts analysed by fluorescence and atomic force microscopy. Biomech Model Mechanobiol. 2006 5 : 263 272.
32. Ingber DE. Tensegrity: the architectural basis of cellular mechanotransduction. Annu Rev Physiol. 1997 59 : 575 599.
33. Ingber DE. Cellular mechanotransduction: putting all the pieces together again. FASEB J. 2006 20 : 811 827.
34. Berdyyeva TK, Woodworth CD, Sokolov I. Human epithelial cells increase their rigidity with aging in vitro: direct measurements. Phys Med Biol. 2005 50 : 81 92.
35. Cytrynbaum EN, Sommi P, Brust-Mascher I, et al. Early spindle assembly in Drosophila embryos: role of a force-balance involving cytoskeletal dynamics and nuclear mechanics. Mol Biol Cell. 2005 16 : 4967 4981.
36. Mounkes L, Kozlov S, Burke B, et al. The laminopathies: nuclear structure meets disease. Curr Op Genet Dev. 2003 13 : 223 230.