Imaging surface and submembranous structures with the atomic force microscope: A study on living cancer cells, fibroblasts and macrophages

Journal of Microscopy

Volumn 190, Issue 3, 1998, Pages 328-338

  Braet, F ^a   Seynaeve, C ^a   De Zanger, R ^a   Wisse, E ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Atomic force microscopy; Colon carcinoma cells; Fibroblasts; Image artefacts; Liver; Macrophages; Scanning electron microscopy

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; ATOMIC FORCE MICROSCOPY; CELL CULTURE; FIBROBLASTS; IMAGE ACQUISITION; MACROPHAGES;

ATOMIC FORCE; ATOMIC-FORCE-MICROSCOPY; CELL-BE; CELL/B.E; CELL/BE; COLON CARCINOMA CELLS; GLUTARALDEHYDES; IMAGE ARTEFACTS; LIVING CELL; MICROSCOPY IMAGING;

SCANNING ELECTRON MICROSCOPY;

GLUTARALDEHYDE;

ANIMAL CELL; ARTICLE; ARTIFACT; ATOMIC FORCE MICROSCOPY; CANCER CELL; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CYTOSKELETON; MACROPHAGE; MICROFILAMENT; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; RAT; SCANNING FORCE MICROSCOPY; SKIN FIBROBLAST; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALIA;

EID: 0031860049     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00333.x     Document Type: Article

References (47)

1. Allen, M.J., Hud, N.V., Balooch, M., Tench, R.J., Siekhaus, W.J. & Balhorn, R. (1992) Tip-radius-induced artifacts in AFM images of protamine-complexed DNA fibers. Ultramicroscopy, 42, 1095-1100.
2. Beckmann, M., Kolb, H.A. & Lang, F. (1994) Atomic force microscopy of peritoneal macrophages after particle phagocytosis. J. Membrane Biol. 140, 197-204.
3. Binnig, G., Quate, C.F. & Gerber, C.H. (1986) Atomic force microscope. Phys. Rev. Lett. 56, 930-953.
4. Bottomley, L.A., Coury, J.E. & First, P.N. (1996) Scanning probe microscopy. Anal. Chem. 68, 185-230.
5. Braet, F., De Zanger, R., Jans, D., Spector, I. & Wisse, E. (1996a) Microfilament disrupting agent latrunculin A induces an increased number of fenestrae in rat liver sinusoidal endothelial cells: comparison with cytochalasin B. Hepatology, 24, 627-635.
6. Braet, F., De Zanger, R., Kalle, W.H.J., Raap, A.K., Tanke, H.J. & Wisse, E. (1996b) Comparative scanning, transmission and atomic force microscopy of the microtubular cytoskeleton in fenestrated endothelial cells. Scanning Microsc. 10, 225-236.
7. Braet, F., De Zanger, R., Kämmer, S. & Wisse, E. (1997a) Noncontact versus contact imaging: an atomic force microscopic study on hepatic endothelial cells in vitro. Int. J. Imaging Syst. Technol. 8, 162-167.
8. Braet, F., De Zanger, R., Sasaoki, T., Baekeland, M., Janssens, P., Smedsrød, B. & Wisse, E. (1994) Assessment of a method of isolation, purification and cultivation of rat liver sinusoidal endothelial cells. Lab. Invest. 70, 944-952.
9. Braet, F., De Zanger, R. & Wisse, E. (1997b) Drying cells for SEM, AFM and TEM by hexamethyldisilazane: a study on hepatic endothelial cells. J. Microsc. 186, 84-87.
10. Braet, F. Kall W.H.J., De Zanger, R.B., de Grooth, B.G., Raap, A.K., Tanke, H.J. & Wisse, E. (1996c) Comparative atomic force and scanning electron microscopy; an investigation on fenestrated endothelial cells in vitro. J. Microsc. 181, 10-17.
11. Braet, F., Rotsch, C., Wisse, E. & Radmacher, M. (1998) Comparison of fixed and living liver endothelial cells by AFM. Appl. Phys. A, in press.
12. Collard, J.G. & Temmink, J.H.M. (1976) Surface morphology and agglutinability with concanavalin A in normal and transformed murine fibroblasts. J. Cell. Biol. 68, 101-112.
13. Freshney, I. (1987) Disaggregation of the tissue and primary culture. Culture of Animal Cells - a Manual of Basic Techniques (ed. by I. Freshney), pp. 107-126. A. Liss, New York.
14. Fritz, M., Radmacher, M. & Gaub, H.E. (1994) Granular motion and membrane spreading during activation of human platelets imaged by atomic force microscopy. Biophys. J. 66, 1328-1334.
15. Grütter, P., Zimmermann-Edling, W. & Brodbeck, D. (1992) Tip artifacts of microfabricated force sensors for atomic force microscopy. Appl. Phys. Lett. 60, 2741-2743.
16. Häberle, W., Hörber, J.K.H. & Binnig, G. (1991) Force microscopy on living cells. J. Vac. Sci. Technol. 9, 1210-1213.
17. Hansma, H.G. & Hoh, J.H. (1994) Biomolecular imaging with the atomic force microscope. Annu. Rev. Biophys. Biomol. Struct. 23, 115-139.
18. Haydon, P.G., Lartius, R., Parpura, V. & Marchese-Ragona, S.P. (1996) Membrane deformation of living glial cells using atomic force microscopy. J. Microsc. 182, 114-120.
19. Henderson, E., Haydon, P.G. & Sakaguchi, D.S. (1992) Actin filament dynamics in living glial cells imaged by atomic force microscopy. Science, 257, 944-946.
20. Hoh, J.H. & Hansma, P.K. (1992) Atomic force microscopy for high-resolution imaging in cell biology. Trends Cell Biol. 2, 208-213.
21. Hoh, J.H. & Schoenenberger, C.A. (1994) Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. J. Cell Sci. 107, 1105-1114.
22. Hörber, J.K.H., Häberle, W., Ohnesorge, F., Binnig, G., Liebich, H.G., Czerny, C.P., Mahnel, H. & Mayr, A. (1992) Investigation of living cells in the nanometer regime with the scanning force microscope. Scanning Microsc. 6, 919-950.
23. Kasas, S., Gotzos, V. & Celio, M.R. (1993) Observation of living cells using atomic force microscope. Biophys. J. 64, 539-544.
24. Kasas, S., Thomson, N.H., Smith, B.L., Hansma, P.K., Miklossy, J. & Hansma, H.G. (1997) Biological applications of the AFM: From single molecules to organs. J. Imaging Syst. Technol. 8, 151-161.
25. Lal, R., Drake, B., Blumberg, D., Saner, D.R., Hansma, P.K. & Feinstein, S.C. (1995) Imaging real-time neurite outgrowth and cytoskeletal reorganization with an atomic force microscope. Am. J. Physiol. 269, C275-C285.
26. Lal, R. & Scott, A.J. (1994) Biological applications of atomic force microscopy. Am. J. Physiol. 266, C1-C21.
27. Laskin, D.L. (1997) Role of hepatic macrophages in inflammation and tissue injury. Functional Heterogeneity of the Liver Tissue (ed. by F. Vidal-Vanaclocha), pp. 161-176. Austin: Medical Intelligence Unit - R. G. Landes Company, London.
28. Lewalle, J.M., Szpirer, C., Szpirer, J., Manaut, C. & Foidart, J.M. (1991) Rat chromosome 5 (q22-23) contains elements that control, cell morphology and interactions with extracellular matrix: a study of normal fibroblast x malignant cell hybrids. Exp. Cell Res. 196, 164-171.
29. Lindroth, M., Bell, P.B., Fredriksson, B.A. & Xiao-Dong, L. (1992) Preservation and visualisation of molecular structure in detergent-extracted whole mounts of cultured cells. Microsc. Res. Techn. 22, 130-150.
30. Macejak, D.G. & Luftig, R.B. (1991) Stabilization of actin filaments at early times after adenovirus infection and in heat-shocked cells. Virus Res. 19, 31-46.
31. Marquet, R.L., Westbroek, D.L. & Jeekel, J. (1984) Interferon treatment of a transplantable rat colon adenocarcinoma: importance of tumor site. Int. J. Cancer. 33, 689-692.
32. Morris, V.J. (1994) Biological applications of scanning probe microscopies. Prog. Biophys. Molec. Biol. 61, 131-185.
33. Proksch, R., Lal, R., Hansma, P.K., Morse, D. & Stucky, G. (1996) Imaging the internal and external pore structure of membranes in fluid: tapping mode scanning ion conductance microscopy. Biophys. J. 71, 2155-2157.
34. Putman, C.A.J., van der Werf, K.O., de Grooth, B.G., van Hulst, N.F. & Greve, J. (1994) Viscoelasticity of living cells allows high resolution imaging by tapping mode atomic force microscopy. Biophys. J. 67, 1749-1753.
35. Putman, C.A.J., van Leeuwen, A.M., de Grooth, B.G., Radosevic, K., van der Werf, K.O., van Hulst, N.F. & Greve, J. (1993) Atomic-force microscopy combined with confocal laser scanning microscopy: a new look at cells. Bioimaging, 1, 63-70.
36. Radmacher, M., Tillman, R.W., Fritz, M. & Gaub, H.E. (1992) From molecules to cells: imaging soft samples with the atomic force microscope. Science, 257, 1900-1905.
37. Rotsch, C., Braet, F., Wisse, E. & Radmacher, M. (1998) AFM imaging and elasticity measurements on living rat liver macrophages. Cell Biol. Int., in press.
38. Schoenenberger, C.A. & Hoh, J.H. (1994) Slow cellular dynamics in MDCK and R5 cells monitored by time-lapse atomic force microscopy. Biophys. J. 67, 929-936.
39. Smedsrød, B., Pertoft, H., Eggertsen, G. & Sundström, C. (1985) Functional and morphological characterization of cultures of Kupffer cells and liver endothelial cells prepared by means of density separation in Percoll, and selective substrate adherence. Cell. Tissue. Res. 241, 639-649.
40. Spector, I., Shochet, N.R., Blasberger, D. & Kashman, Y. (1989) Latrunculins - novel marine macrolides that disrupt microfilament organization and affect cell growth: comparison with cytochalasin D. Cell Motil. Cytoskel. 13, 127-144.
41. Spector, I., Shochet, N.R., Kashman, Y. & Groweiss, A. (1983) Latrunculins: novel marine toxins that disrupt microfilament organization in cultured cells. Sci. 219, 491-495.
42. Thomas, C., Nijenhuis, A.M., Timens, W., Kuppen, P.J.K., Daemen, T. & Scherphof, G.L. (1993) Liver metastasis model of colon cancer in the rat: Immunohistochemical characterization. Invas. Metast. 13, 102-112.
43. Ushiki, T., Hitomi, J., Ogura, S., Umemoto, T. & Shigeno, M. (1996) Atomic force microscopy in histology and cytology. Arch. Histol. Cytol. 59, 421-451.
44. Vermijlen, D., Braet, F., Jans, D., Luo, D., De Zanger, R. & Wisse, E. (1997) Observations on the actin cytoskelelon of pit cells and CC531s colon carcinoma cells during cytotoxic conjugation. Cells of the Hepatic Sinusoid 6 (ed. by K. Wisse, D. L. Knock, C. Balabaud), pp. 429-454. Kupffer Cell Foundation, Leiden.
45. Wisse, E., Braet, F., Luo, D., De Zanger, R., Jans, D., Crabbé, E. & Vermoesen, A. (1996) Structure and function of sinusoidal lining cells in the liver. Toxicol. Pathol. 24, 100-111.
46. Yang, J. & Zhifeng, S. (1995) Recent advances in biological atomic force microscopy. Micron, 26, 55-49.
47. Zhang, P.C., Bai,C., Huang, Y.-M., Zhao, H., Fang, Y., Wang, N.-X. & Li, Q. (1995) Atomic force microscopy of line structures of the entire surface of red blood cells. Scanning Microsc. 9, 981-988.