Comparative study of the conditions required to image live human epithelial and fibroblast cells using atomic force microscopy

Microscopy Research and Technique

Volumn 69, Issue 9, 2006, Pages 757-765

  Murphy, Mark F ^a   Lalor, Michael J ^a   Manning, Francis C R ^a   Lilley, Francis ^a   Crosby, Steven R ^a   Randall, Catherine ^a   Burton, David R ^a  

^a LIVERPOOL JOHN MOORES UNIVERSITY   (United Kingdom)

Author keywords

Atomic force microscope; Epithelial; Fibroblast; Human; Live cells

Indexed keywords

ATOMIC FORCE MICROSCOPY; CELL CULTURE; NANOCANTILEVERS; SPRINGS (COMPONENTS); SURFACE TOPOGRAPHY;

ATOMIC FORCE; ATOMIC FORCE MICROSCOPE; ATOMIC-FORCE-MICROSCOPY; CELL CONTACTS; COMPARATIVES STUDIES; CONTACT MODES; EPITHELIAL; HUMAN; LIVE CELL; SPRING CONSTANTS;

FIBROBLASTS;

POLYLYSINE;

ARTICLE; ATOMIC FORCE MICROSCOPY; CELL CULTURE; CELL STRUCTURE; CELL SURFACE; CELL TYPE; COMPARATIVE STUDY; CONTROLLED STUDY; ELECTRON MICROSCOPY; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; IMAGE QUALITY; IMAGE RECONSTRUCTION; PRIORITY JOURNAL;

EID: 33748698033     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20339     Document Type: Article

References (23)

1. Binnig G, Quate CF, Gerber C. 1986. Atomic force microscope. Phys Rev Lett 56:930-933.
2. Bushell GR, Cahill C, Clarke FM, Gibson CT, Myhra S, Watson GS. 1999. Analysis of human fibroblast in vitro-Imaging conditions and cytochalasin treatment. Appl Surf Sci 144/145:141-145.
3. Campbell PA, Cuschieri A, Liovic M, Lane EB. 2001. Cytoskeleton metrology and dynamics during cell motility: AFM observation of PtK2 cells in the pico-newton force regime. Inst Phys Conf Ser 168:517-520.
4. Engel A, Schoenenberger CA, Müller DJ. 1997. High resolution imaging of native biological sample surfaces using scanning probe microscopy. Curr Opin Struct Biol 7:279-284.
5. Hansma HG, Revenko I, Kim K, Laney DE. 1996. Atomic force microscopy of long and short double-stranded, single-stranded and triple-stranded nucleic acids. Nucleic Acids Res 24:713-720.
6. Henderson E, Haydon PG, Sakaguchi DS. 1992. Actin filament dynamics in living glial cells imaged by atomic force microscopy. Science 257:1944-1946.
7. Hofmann UG, Rotsch C, Parak WJ, Radmacher MJ. 1997. Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope. Struct Biol 119:84-91.
8. Hoh JH, Schoenenberger CA. 1994. Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. J Cell Sci 107:1105-1114.
9. Kim JM, Jung HO, Park JW, Lee HY, Kawai T. 2004. AFM phase lag mapping for protein-DNA oligonucleotide complexes. Anal Chim Acta 525:151-157.
10. Le Grimellec C, Lesniewska É, Cachia C, Schreiber JP, de Fornel F, Goudonnet JP. 1994. Imaging of the membrane surface of MDCK cells by atomic force microscopy. Biophys J 67:36-41.
11. Le Grimellec C, Lesniewska É, Giocondi MĆ, Finot É, Goudonnet JP. 1997. Simultaneous imaging of the surface and the submembranous cytoskeleton in living cells by tapping mode atomic force microscopy. C R Acad Sci III 320:637-643.
12. Le Grimellec C, Lesniewska É, Giocondi MĆ, Finot É, Vié V, Goudonnet JP. 1998. Imaging of the surface of living cells by low-force contact-mode atomic force microscopy. Biophys J 75:695-703.
13. Mücke N, Kreplak L, Kirmse R, Wedig T, Herrmann H, Aebi U, Langowski J. 2004. Assessing the flexibility of intermediate filaments by atomic force microscopy. J Mol Biol 335:1241-1250.
14. Nagao E, Dvorak JA. 1998. An integrated approach to the study of living cells by atomic force microscopy. J Microsc 191:8-19.
15. Pu FR, Manning FCR, Brannigan AE, Crosby SR. 2001. Differential regulation of calcitonin secretion in normal and neoplastic pulmonary neuroendocrine cells in vitro. Exp Lung Res 27:689-703.
16. Putman CAJ, van der Werf KO, de Grooth BG, van Hulst NF, Greve J. 1994. Viscoelasticity of living cells allows high-resolution imaging by tapping mode atomic-force microscopy. Biophys J 67:1749-1753.
17. Rotsch C, Braet F, Wisse E, Radmacher M. 1997. AFM imaging and elasticity measurements on living rat macrophages. Cell Biol Int 21:685-696.
18. Rotsch C, Jacobson K, Radmacher M. 1999. Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by atomic force microscopy. Proc Natl Acad Sci USA 96:921-926.
19. Schaus SS, Henderson ER. 1997. Cell viability and probe-cell membrane interactions of XR1 glial cells imaged by atomic force microscopy. Biophys J 73:1205-1214.
20. Scheuring S, Seguin J, Marco S, Lévy D, Robert B, Rigaud JL. 2003. Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Proc Natl Acad Sci USA 100:1690-1693.
21. Shlyakhtenko LS, Gall AA, Weimer JJ, Hawn DD, Lyubchenko YL. 1999. Atomic force microscopy imaging of DNA covalently immobilized on a functionalized mica substrate. Biophys J 77:568-576.
22. Stark RW, Heckl WM. 2003. Higher harmonics imaging in tapping-mode atomic-force microscopy. Rev Sci Instrum 74:5111-5114.
23. Vié V, Giocondi MĆ, Lesniewska É, Finot É, Goudonnet JP, Le Grimellec C. 2000. Tapping-mode atomic force microscopy on intact cells: Optimal adjustment of tapping conditions by using the deflection signal. Ultramicroscopy 82:279-288.