Analysis of integral membrane proteins by heat gel-embedment combined with improved in-gel digestions

Electrophoresis

Volumn 30, Issue 23, 2009, Pages 4109-4117

  Zhou, Jian ^a   Huang, Sha ^a   Bi, Deng ^a   Zhang, Hai ^a   Li, Jianglin ^a   Lin, Yong ^a   Chen, Ping ^a   Wang, Xianchun ^a   Liang, Songping ^a  

^a HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Gel embedment; In gel digestion; Integral membrane protein; Mass spectrometry; Polyacrylamide

Indexed keywords

ACTIVATION ANALYSIS; BIOLOGICAL MEMBRANES; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; PROTEINS; SOAPS (DETERGENTS); SODIUM;

ACRYLAMIDES; CAPILLARY LIQUID CHROMATOGRAPHY; GEL-EMBEDMENT; HYDROPHOBIC NATURE; INGEL DIGESTION; INTEGRAL MEMBRANE PROTEINS; LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROMETRIES; MEMBRANE PROTEINS; PROTEIN LYSATES; SODIUM DEOXYCHOLATE;

MASS SPECTROMETRY;

DEOXYCHOLATE SODIUM; MEMBRANE PROTEIN; POLYACRYLAMIDE GEL; TRYPSIN;

ANALYTIC METHOD; ANIMAL CELL; CAPILLARY CHROMATOGRPHY; CHEMICAL PROCEDURES; CONFERENCE PAPER; HEAT GEL EMBEDMENT; HIGH THROUGHPUT SCREENING; HYDROPHOBICITY; IN GEL DIGESTION; LIQUID CHROMATOGRAPHY; NONHUMAN; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; RAT; SOLVENT EFFECT; TANDEM MASS SPECTROMETRY; TEMPERATURE SENSITIVITY;

EID: 72849131098     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900391     Document Type: Conference Paper

References (22)

1. Speers, A. E., Wu, C. C., Chem. Rev. 2007, 107, 3687-3714.
2. Hopkins, A. L., Groom, C. R., Nat. Rev. Drug Discov. 2002, 1, 727-730.
3. Zhang, L. J., Wang, X. E., Peng, X., Wei, Y. J., Cao, R., Liu, Z., Xiong, J. X. et al., Cell. Mol. Life Sci. 2006, 63, 1790-1804.
4. Josic, D., Clifton, J. G., Proteomics 2007, 7, 3010-3029.
5. Fischer, F., Wolters, D., Rögner, M., Poetsch, A., Mol. Cell. Proteomics 2006, 5, 444-453.
6. Zhang, N., Chen, R., Young, N., Wishart, D., Winter, P., Weiner, J. H., Li, L., Proteomics 2007, 7, 484-493.
7. McCarthy, F. M., Burgess, S. C., van den Berg, B. H. J., Koter, M. D., Pharr, G. T., J. Proteome Res. 2005, 4, 316-324.
8. Nagaraj, N., Lu, A., Mann, M., Wiśniewski, J. R., J. Proteome Res. 2008, 7, 5028-5032.
9. Lu, X., Zhu, H., Mol. Cell. Proteomics 2005, 4, 1948-1958.
10. Yu, H., Wakim, B., Li, M., Halligan, B., Tint, G. S., Patel, S. B., Proteome Sci. 2007, 5, 17.
11. Han, C. L., Chien, C. W., Chen, W. C., Chen, Y. R., Wu, C. P., Li, H., Chen, Y. J., Mol. Cell. Proteomics 2008, 7, 1983-1997.
12. Krogh, A., Larsson, B., von Heijne, G., Sonnhammer, E. L., J. Mol. Biol. 2001, 305, 567-580.
13. Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P. et al., Nat. Genet. 2000, 25, 25-29.
14. Blonder, J., Goshe, M. B., Moore, R. J., Pasa-Tolic, L., Masselon, C. D., Lipton, M. S., Smith, R. D., J. Proteome Res. 2002, 1, 351-360.
15. Chen, E. I., Cociorva, D., Norris, J. L., Yates, J. R., 3rd., J. Proteome Res. 2007, 6, 2529-2538.
16. Zhang, N., Li, L., Rapid Commun. Mass Spectrom. 2004, 18, 889-896.
17. Nomura, E., Katsuta, K., Ueda, T., Toriyama, M., Mori, T., Inagaki, N. J., J. Mass Spectrom. 2004, 39, 202-207.
18. Ruth, M. C., Old, W. M., Emrick, M. A., Meyer-Arendt, K., Aveline-Wolf, L. D., Pierce, K. G., Mendoza, A. M. et al., J. Proteome Res. 2006, 5, 709-719.
19. Zhou, J., Zhou, T., Cao, R., Liu, Z., Shen, J., Chen, P., Wang, X. et al., J. Proteome Res. 2006, 5, 2547-2553.
20. Masuda, T., Tomita, M., Ishihama, Y., J. Proteome Res. 2008, 7, 731-740.
21. Zhang, L., Xie, J., Wang, X., Liu, X., Tang, X., Cao, R., Hu, W. et al., Proteomics 2005, 5, 4510-4524.
22. Chen, P., Li, X., Sun, Y., Liu, Z., Cao, R., He, Q., Wang, M. et al., J. Neurochem. 2006, 98, 1126-1140.