NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: Histidine 188 is the general base

Biochemistry

Volumn 48, Issue 47, 2009, Pages 11220-11230

  Chan, Patrick H W ^a   Lairson, Luke L ^a   Lee, Ho Jun ^a   Wakarchuk, Warren W ^b   Strynadka, Natalie C J ^a,c   Withers, Stephen G ^a   McIntosh, Lawrence P ^a,c  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CAMPYLOBACTER JEJUNI; CATALYTIC BASE; CATALYTIC ROLE; CELL SURFACES; CONFORMATIONAL EXCHANGE; DEUTERATED PROTEINS; GENERAL BASE; GLYCANS; MONOMERIC FORMS; MONOMERIC VARIANTS; NATURAL SUGARS; NMR SPECTROSCOPY; PH DEPENDENCE; PH-DEPENDENT; POINT MUTATIONS; SIALIC ACIDS; SIALYLTRANSFERASES; SITE-SPECIFIC; SPECTROSCOPIC CHARACTERIZATION; STRUCTURE AND DYNAMICS; SUBUNIT INTERFACES; SUGAR ACCEPTORS; TETRAMERS; TIME-SCALES; TRANSFER MECHANISMS; WILD TYPES;

AMIDES; CARBOXYLIC ACIDS; CELL MEMBRANES; DEUTERIUM; DYES; ENZYMES; NANOTECHNOLOGY; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SUGAR (SUCROSE); SUGARS;

CRYSTAL STRUCTURE;

SIALYLTRANSFERASE;

ARTICLE; CAMPYLOBACTER JEJUNI; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ANALYSIS; ENZYME STRUCTURE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POINT MUTATION; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ANIONS; CAMPYLOBACTER JEJUNI; CATALYSIS; HISTIDINE; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; POINT MUTATION; SIALYLTRANSFERASES; SUBSTRATE SPECIFICITY;

CAMPYLOBACTER JEJUNI;

EID: 72749096483     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi901606n     Document Type: Article

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