Molecular dissection of membrane-transport proteins: Mass spectrometry and sequence determination of the galactose-H+ symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine and 15NH3

Biochemical Journal

Volumn 363, Issue 2, 2002, Pages 243-252

  Venter, Henrietta ^a   Ashcroft, Alison E ^a   Keen, Jeffrey N ^a   Henderson, Peter J F ^a   Herbert, Richard B ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

CNBr proteolysis; Glucose transporter; NMR; Stable isotope labelling; Trypsin proteolysis

Indexed keywords

AMINO ACIDS; DESORPTION; DNA SEQUENCES; ELECTROPHORESIS; IONIZATION; LASER APPLICATIONS; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; SIZE EXCLUSION CHROMATOGRAPHY;

MEMBRANE-TRANSPORT PROTEINS;

PROTEINS;

CARRIER PROTEIN; GALACTOSE; GLUCOSE TRANSPORTER; HISTIDINE; MEMBRANE PROTEIN; METHIONINE; NITRILOTRIACETIC ACID; PROTEIN GALP; TRYPSIN; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; DNA SEQUENCE; ELECTROPHORESIS; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEASUREMENT; MOLECULAR WEIGHT; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PURIFICATION; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; AMMONIA; CALCIUM-BINDING PROTEINS; CARBON ISOTOPES; CYANOGEN BROMIDE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GALACTOSE; HISTIDINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MONOSACCHARIDE TRANSPORT PROTEINS; NITROGEN ISOTOPES; PEPTIDE FRAGMENTS; PERIPLASMIC BINDING PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

ESCHERICHIA COLI;

EID: 0037090565     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3630243     Document Type: Article

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