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Volumn 7, Issue 24, 2009, Pages 5245-5254

Assessing the cluster glycoside effect during the binding of concanavalin A to mannosylated artificial lipid rafts

Author keywords

[No Author keywords available]

Indexed keywords

CONCANAVALIN A; GLYCOLIPIDS; LIPID RAFT; MEMBRANE ANCHORS; MEMBRANE LOADING; MULTIVALENT BINDING;

EID: 72549113665     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/b910976e     Document Type: Article
Times cited : (35)

References (56)
  • 2
    • 68149100633 scopus 로고    scopus 로고
    • Cambridge University Press, New York, 1st edn
    • M. Luckey, in Membrane Structural Biology, Cambridge University Press, New York, 1st edn, 2008, ch. 4, pp. 73-76
    • (2008) Membrane Structural Biology , pp. 73-76
    • Luckey In, M.1
  • 22
    • 85184245152 scopus 로고
    • Although there are significant differences in E/M between 2 and 3, it is difficult to compare E/M values for different pyrenyl compounds if E/M is already large, i.e. monomer emission is weak. E/M is sensitive to several factors including excited-state lifetime and the rate of collision between pyrene moieties. See ref. 33
    • G. Zemplén E. Pacsu Chem. Ber. 1929 62 1613
    • (1929) Chem. Ber. , vol.62 , pp. 1613
    • Zemplén, G.1    Pacsu, E.2
  • 41
    • 0016846985 scopus 로고
    • This effect means changes in E/M ratio will not necessarily reflect changes in lateral distribution if the multivalent ligand causes significant dynamic quenching. See ref. 32 Replicating the procedure of Bondurant et al. (ref. 13) resulted in a broad and featureless pyrene emission spectrum. We believe the large excess of Con A compared to phospholipid and mannose lipid (a 1:1 ratio of Con A to total lipid) may cause rapid bilayer disruption, similar to that observed by Bondurant et al. over several hours
    • H. J. Galla E. Sackmann J. Am. Chem. Soc. 1975 97 4114
    • (1975) J. Am. Chem. Soc. , vol.97 , pp. 4114
    • Galla, H.J.1    Sackmann, E.2
  • 42
    • 0003475878 scopus 로고    scopus 로고
    • Wiley-VCH, Weinheim, 1st edn, 86 The quenching constants obtained from these Stern-Volmer plots were verified by iterative fitting of the data to a 1+1 binding isotherm in Dynafit. See
    • B. Valeur, in Molecular Fluorescence: Principles and Applications, Wiley-VCH, Weinheim, 1st edn, 2002, ch. 4, p. 86
    • (2002) Molecular Fluorescence: Principles and Applications
    • Valeur In, B.1
  • 50
    • 28944446243 scopus 로고    scopus 로고
    • These domains have a comparable size to those observed for other phase-separating pyrenyl lipids. See
    • C. W. Lim B. J. Ravoo D. N. Reinhoudt Chem. Commun. 2005 5627
    • (2005) Chem. Commun. , pp. 5627
    • Lim, C.W.1    Ravoo, B.J.2    Reinhoudt, D.N.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.