Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus

Clinical Chemistry

Volumn 55, Issue 12, 2009, Pages 2218-2222

  Wenzel, Jürgen J ^a   Walch, Heiko ^b   Bollwein, Markus ^a   Niller, Hans Helmut ^a   Ankenbauer, Waltraud ^b   Mauritz, Ralf ^b   Höltke, Hans Joachim ^b   Zepeda, Héctor Manuel ^c   Wolf, Hans ^a   Jilg, Wolfgang ^a   Reischl, Udo ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b ROCHE DIAGNOSTICS GMBH   (Germany)

^c INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; HEMAGGLUTININ; LOCKED NUCLEIC ACID; NUCLEIC ACID; SIALIDASE;

ARTICLE; CLINICAL EVALUATION; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC TEST; EUROPE; FLUORESCENCE; GENE LIBRARY; GERMANY; HUMAN; HUMAN CELL; HYDROLYSIS; IMMUNOASSAY; INFLUENZA VIRUS A; INFLUENZA VIRUS A H1N1; MEXICO; MOLECULAR PROBE; NONHUMAN; NUCLEIC ACID SYNTHESIS; NUCLEOTIDE SEQUENCE; PATHOGENESIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS STRAIN;

ANIMALS; CROSS REACTIONS; FEASIBILITY STUDIES; FLUORESCENT DYES; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; HUMANS; HYDROLYSIS; INFLUENZA A VIRUS, H1N1 SUBTYPE; INFLUENZA, HUMAN; NEURAMINIDASE; NUCLEIC ACID PROBES; OLIGONUCLEOTIDES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY;

ANIMALIA; INFLUENZA A VIRUS; ORTHOMYXOVIRIDAE;

EID: 71749087119     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2009.136192     Document Type: Article

References (17)

1. Update: swine influenza A (H1N1) infections: California and Texas, April 2009. MMWR Morb Mortal Wkly Rep 2009;58:435-437
2. WHO. World now at the start of 2009 influenza pandemic. 2009. http://www.who.int/mediacentre/news/statements/2009/h1n1-pandemic-phase6- 20090611/en/index.html (Accessed August 2009).
3. Neumann G, Noda T, Kawaoka Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature 2009;459:931-939
4. Dawood FS, Jain S, Finelli L, Shaw MW, Lindstrom S, Garten RJ, et al. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 2009;360:2605-2615
5. Taubenberger JK, Morens DM. 1918 Influenza: the mother of all pandemics. Emerg Infect Dis 2006;12:15-22.
6. Petric M, Comanor L, Petti CA. Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemics. J Infect Dis 2006;194 Suppl 2:S98-110. (Pubitemid 44630953)
7. Melzl H, Wenzel JJ, Kochanowski B, Feierabend K, Kreuzpaintner B, Kreuzpaintner E, et al. First sequence-confirmed case of infection with the new influenza A(H1N1) strain in Germany. Euro Surveill 2009;14(18):3-4; erratum 2009;14(19). Erratum available online.
8. Panning M, Eickmann M, Landt O, Monazahian M,Ölschläger S, Baumgarte S, et al. Detection of influenza A(H1N1)v virus by real-time RT-PCR. Euro Surveill. 2009;14(36):7-12.
9. Braasch DA, Corey DR. Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA. Chem Biol 2001;8:1-7. (Pubitemid 32165807)
10. Petersen M, Wengel J. LNA: a versatile tool for therapeutics and genomics. Trends Biotechnol 2003;21:74-81. (Pubitemid 36165007)
11. Wahlestedt C, Salmi P, Good L, Kela J, Johnsson T, Hokfelt T, et al. Potent and nontoxic antisense oligonucleotides containing locked nucleic acids. Proc Natl Acad Sci U S A 2000;97:5633-5638 (Pubitemid 30313770)
12. Update: novel influenza A (H1N1) virus infection: Mexico, March-May, 2009. MMWR Morb Mortal Wkly Rep 2009;58:585-589
13. Chan KH, Lai ST, Poon LL, Guan Y, Yuen KY, Peiris JS. Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1). J Clin Virol 2009;45:205-207
14. Carr MJ, Gunson R, Maclean A, Coughlan S, Fitzgerald M, Scully M, et al. Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections. J Clin Virol 2009;45:196-199
15. Lau SK, Chan KH, Yip CC, Ng TK, Tsang OT, Woo PC, Yuen KY. Confirmation of the first Hong Kong case of human infection by novel swine origin influenza A (H1N1) virus diagnosed using ultrarapid, real-time reverse transcriptase PCR. J Clin Microbiol 2009;47:2344-2346
16. Poon LL, Chan KH, Smith GJ, Leung CS, Guan Y, Yuen KY, Peiris JS. Molecular detection of a novel human influenza (H1N1) of pandemic potential by conventional and real-time quantitative RTPCR assays. Clin Chem 2009;55:1555-1558
17. Ward CL, Dempsey MH, Ring CJ, Kempson RE, Zhang L, Gor D, et al. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J Clin Virol 2004;29:179-188 (Pubitemid 38201464)