3D tomography reveals connections between the phagophore and endoplasmic reticulum

Autophagy

Volumn 5, Issue 8, 2009, Pages 1180-1185

  Ylä Anttila, Päivi ^a   Vihinen, Helena ^b   Jokitalo, Eija ^b   Eskelinen, Eeva Liisa ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

Author keywords

3D reconstruction; Autophagosome; Electron microscopy; Electron tomography; Endoplasmic reticulum; Isolation membrane; Phagophore

Indexed keywords

FAT DROPLET; GLUTARALDEHYDE; OSMIUM TETRAOXIDE;

ANIMAL CELL; ARTICLE; AUTOPHAGOSOME; BILAYER MEMBRANE; CELL ORGANELLE; ELECTRON MICROSCOPY; KIDNEY CELL; MEMBRANE FUSION; MOUSE; NONHUMAN; PHAGOPHORE; ROUGH ENDOPLASMIC RETICULUM; THREE DIMENSIONAL IMAGING; TOMOGRAPHY;

RATTUS;

EID: 71649112895     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.5.8.10274     Document Type: Article

References (22)

1. Eskelinen EL. New insights into the mechanisms of macroautophagy in mammalian cells. Int Rev Cell Mol Biol 2008; 266:207-47.
2. Seglen PO. Regulation of autophagic protein degradation in isolated liver cells. In: Glaumann H, Ballard FJ, ed. Lysosomes: Their role in protein breakdown. Orlando, London 1987; 371-414.
3. Yamamoto A, Masaki R, Fukui Y, Tashiro Y. Absence of cytochrome p-450 and presence of autolysosomal membrane antigens on the isolation membranes and autophagosomal membranes in rat hepatocytes. J Histochem Cytochem 1990; 38:1571-81.
4. Locke M, Sykes AK. The role of the golgi complex in the isolation and digestion of organelles. Tissue Cell 1975; 7:143-58.
5. Juhasz G, Neufeld TP. Autophagy: A forty-year search for a missing membrane source. PLoS Biol 2006; 4:36.
6. Eskelinen EL. The mystery of the membranes. Autophagy 2008; 4:3-4.
7. Longatti A, Tooze SA. Vesicular trafficking and autophagosome formation. Cell Death Differ 2009; 16:956-65.
8. Ishihara N, Hamasaki M, Yokota S, Suzuki K, Kamada Y, Kihara A, et al. Autophagosome requires specific early sec proteins for its formation and nsf/snare for vacuolar fusion. Mol Biol Cell 2001; 12:3690-702.
9. Arstila AU, Trump BF. Studies on cellular autophagocytosis. The formation of autophagic vacuoles in the liver after glucagon administration. Am J Pathol 1968; 53:687-733.
10. Eskelinen EL. Macroautophagy in mammalian cells. In: Saftig P, ed. Lysosomes. Georgetown: Landes Bioscience/Eurekah.com 2004; 166-80.
11. Kovacs AL, Palfia Z, Rez G, Vellai T, Kovacs J. Sequestration revisited: Integrating traditional electron microscopy, de novo assembly and new results. Autophagy 2007; 3:655-62.
12. Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, et al. Dissection of autophagosome formation using apg5-deficient mouse embryonic stem cells. J Cell Biol 2001; 152:657-67.
13. Fujita N, Hayashi-Nishino M, Fukumoto H, Omori H, Yamamoto A, Noda T, Yoshimori T. An atg4b mutant hampers the lipidation of lc3 paralogues and causes defects in autophagosome closure. Mol Biol Cell 2008; 19:4651-9.
14. Dunn WA. Studies on the mechanisms of autophagy: Formation of the autophagic vacuole. J Cell Biol 1990; 110:1923-33.
15. Furuno K, Ishikawa TKA, Lee S, Nishimura Y, Tsuji H, Himeno M, Kato K. Immunocyto-chemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes. Exp Cell Res 1990; 189:261-8.
16. Axe EL, Walker SA, Manifava M, Chandra P, Roderick HL, Habermann A, et al. Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum. J Cell Biol 2008; 182:685-701.
17. Novikoff AB, Shin WY. Endoplasmic reticulum and autophagy in rat hepatocytes. Proc Natl Acad Sci USA 1978; 75:5039-42.
18. Yla-Anttila P, Vihinen H, Jokitalo E, Eskelinen EL. Monitoring autophagy by electron microscopy in mammalian cells. Methods Enzymol 2009; 452:143-64.
19. Shibata M, Yoshimura K, Furuya N, Koike M, Ueno T, Komatsu M, et al. The map1-lc3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun 2009; 382:419-23.
20. Singh R, Kaushik S, Wang Y, Xiang Y, Novak I, Komatsu M, et al. Autophagy regulates lipid metabolism. Nature 2009; 458:1131-5.
21. Walker S, Chandra P, Manifava M, Axe E, Ktistakis NT. Making autophagosomes: Localized synthesis of phosphatidylinositol 3-phosphate holds the clue. Autophagy 2008; 4:1093-6.
22. Fengsrud M, Roos N, Berg T, Liou W, Slot JW, Seglen PO. Ultrastructural and immunocyto-chemical characterization of autophagic vacuoles in isolated hepatocytes: Effects of vinblastine and asparagine on vacuole distributions. Exp Cell Res 1995; 221:504-19.