Identification of novel bioactive hexapeptides against phytopathogenic bacteria through rapid screening of a synthetic combinatorial library

Journal of Microbiology and Biotechnology

Volumn 19, Issue 8, 2009, Pages 792-802

  Choi, Jaehyuk ^a   Moon, Eunpyo ^a  

^a Ajou University   (South Korea)

Author keywords

AMP; Antibiotics; Antimicrobial peptide; Cytotoxicity; Infection control; MTT test; Phytopathogenic bacteria; Synthetic

Indexed keywords

HEXAPEPTIDE; LYSYLTRYPTOPHANYLTRYPTOPHANYLTRYPTOPHANYLARGINYLTRYPTOPHANYLAMIDE; LYSYLTRYTOPHANYLARGINYLTRYPTOPHANYLISOLEUCYLTRYPTOPHANYLAMIDE; POLYPEPTIDE ANTIBIOTIC AGENT; THREONYLTRYPTOPHANYLTRYPTOPHANYLARGINYLTRYPTOPHANYLTRYPTOPHANYLAMIDE; TRYPTOPHANYLARGINYLTRYPTOPHANYLPHENYLALANYLISOLEUCYLHISTIDYLAMIDE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; BACTERIAL PLANT DISEASE; BACTERIAL STRAIN; BACTERICIDAL ACTIVITY; CABBAGE; COMBINATORIAL LIBRARY; CONTROLLED STUDY; CYTOTOXICITY TEST; EMBRYO; FEMALE; FUNGAL STRAIN; GROWTH INHIBITION; HUMAN; HUMAN CELL; IN VIVO STUDY; INFECTION CONTROL; INOCULATION; MOUSE; NONHUMAN; PECTOBACTERIUM; PHENOTYPE; PLANT DISEASE CONTROL; PSEUDOMONAS; XANTHOMONAS;

ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; BRASSICA RAPA; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; HUMANS; MICE; MICROBIAL SENSITIVITY TESTS; PECTOBACTERIUM; PEPTIDE LIBRARY; PLANT DISEASES; PSEUDOMONAS; XANTHOMONAS;

BACTERIA (MICROORGANISMS); BRASSICA OLERACEA VAR. CAPITATA; PECTOBACTERIUM; PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM; PROTOFUNGI; PSEUDOMONAS; XANTHOMONAS;

EID: 70849087269     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0809.497     Document Type: Article

References (37)

1. Agrios, G. N., 2005. Plant Pathology, 4th Ed. Academic Press, London.
2. Bader, M. W., S. Sanowar, M. E. Daley, A. R. Schneider, U. Cho, W. Xu, R. E. Klevit, H. Le Moual, and S. I. Miller. 2005. Recognition of antimicrobial peptides by a bacterial sensor kinase. Cell 122: 461-472. (Pubitemid 41127146)
3. Blondelle, S. E., E. Crooks, R. Aligue, N. Agell, O. Bachs, V. Esteve, R. Tejero, B. Celda, M. T. Pastor, and E. Perez-Paya. 2000. Novel, potent calmodulin antagonists derived from an all- D hexapeptide combinatorial library that inhibits in vivo cell proliferation: Activity and structural characterization. J. Pept. Res. 55: 148-162.
4. Blondelle, S. E. and R. A. Houghten. 1996. Novel antimicrobial compounds identified using synthetic combinatorial library technology. Trends Biotechnol. 14: 60-65.
5. Blondelle, S. E. and K. Lohner. 2000. Combinatorial libraries: A tool to design antimicrobial and antifungal peptide analogues having lytic specificities for structure-activity relationship studies. Biopolymers 55: 74-87.
6. Blondelle, S. E., E. Perez-Paya, and R. A. Houghten. 1996. Synthetic combinatorial libraries: Novel discovery strategy for identification of antimicrobial agents. Antimicrob. Agents Chemother. 40: 1067-1071.
7. Blondelle, S. E., C. Pinilla, and C. Boggiano. 2003. Synthetic combinatorial libraries as an alternative strategy for the development of novel treatments for infectious diseases. Methods Enzymol. 369: 322-344.
8. Brogden, K. A. 2005. Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nat. Rev. Microbiol. 3: 238-250.
9. Cabrefiga, J. and E. Montesinos. 2005. Analysis of Aggressiveness of Erwinia amylovora Using Disease-Dose and Time Relationships. p. 1430-1437.
10. Chen, Y., M. T. Guarnieri, A. I. Vasil, M. L. Vasil, C. T. Mant, and R. S. Hodges. 2007. Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptides. Antimicrob. Agents Chemother. 51: 1398-1406.
11. Feng, Y., N. Huang, Q. Wu, L. Bao, and B. Y. Wang. 2005. Alpha-helical domain is essential for antimicrobial activity of high mobility group nucleosomal binding domain 2 (HMGN2). Acta Pharmacol. Sin. 26: 1087-1092. (Pubitemid 41296949)
12. Fields, G. B. and R. L. Noble. 1990. Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids. Int. J. Pept. Protein Res. 35: 161-214.
13. Hancock, R. E. and H. G. Sahl. 2006. Antimicrobial and hostdefense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 24: 1551-1557.
14. Heu, S., J. Oh, Y. Kang, S. Ryu, S. K. Cho, Y. Cho, and M. Cho. 2001. gly Gene cloning and expression and purification of glycinecin A, a bacteriocin produced by Xanthomonas campestris pv. glycines 8ra. Appl. Environ. Microbiol. 67: 4105-4110.
15. Hong, S. Y., J. E. Oh, M. Kwon, M. J. Choi, J. H. Lee, B. L. Lee, H. M. Moon, and K. H. Lee. 1998. Identification and characterization of novel antimicrobial decapeptides generated by combinatorial chemistry. Antimicrob. Agents Chemother. 42: 2534-2541.
16. Houghten, R. A. 2000. Parallel array and mixture-based synthetic combinatorial chemistry: Tools for the next millennium. Annu. Rev. Pharmacol. Toxicol. 40: 273-282.
17. Hughes, S. R., P. F. Dowd, R. E. Hector, T. Panavas, D. E. Sterner, N. Qureshi, et al. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces cerevisiae strain. J. Pept. Sci. 14: 1039-1050.
18. Kyte, J. and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105-132.
19. Lopez-Garcia, B., L. Gonzalez-Candelas, E. Perez-Paya, and J. F. Marcos. 2000. Identification and characterization of a hexapeptide with activity against phytopathogenic fungi that cause postharvest decay in fruits. Mol. Plant Microbe Interact. 13: 837-846.
20. Lopez-Garcia, B., J. F. Marcos, C. Abad, and E. Perez-Paya. 2004. Stabilisation of mixed peptide/lipid complexes in selective antifungal hexapeptides. Biochim. Biophys. Acta 1660: 131-137.
21. Lopez-Garcia, B., E. Perez-Paya, and J. F. Marcos. 2002. Identification of novel hexapeptides bioactive against phytopathogenic fungi through screening of a synthetic peptide combinatorial library. Appl. Environ. Microbiol. 68: 2453-2460.
22. Lopez-Garcia, B., W. Ubhayasekera, R. L. Gallo, and J. F. Marcos. 2007. Parallel evaluation of antimicrobial peptides derived from the synthetic PAF26 and the human LL37. Biochem. Biophys. Res. Commun. 356: 107-113.
23. Marcos, J. F., R. N. Beachy, R. A. Houghten, S. E. Blondelle, and E. Perez-Paya. 1995. Inhibition of a plant virus infection by analogs of melittin. Proc. Natl. Acad. Sci. U.S.A. 92: 12466-12469.
24. Matsuzaki, K. 1999. Why and how are peptide-lipid interactions utilized for self-defense? Magainins and tachyplesins as archetypes. Biochim. Biophys. Acta 1462: 1-10.
25. Montesinos, E. 2007. Antimicrobial peptides and plant disease control. FEMS Microbiol. Lett. 270: 1-11.
26. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63. (Pubitemid 14203433)
27. Munoz, A., B. Lopez-Garcia, E. Perez-Paya, and J. F. Marcos. 2007. Antimicrobial properties of derivatives of the cationic tryptophan-rich hexapeptide PAF26. Biochem. Biophys. Res. Commun. 354: 172-177.
28. Ostresh, J. M., G. M. Husar, S. E. Blondelle, B. Dorner, P. A. Weber, and R. A. Houghten. 1994. "Libraries from libraries": Chemical transformation of combinatorial libraries to extend the range and repertoire of chemical diversity. Proc. Natl. Acad. Sci. U.S.A. 91: 11138-11142.
29. Rajasekaran, K., J. W. Cary, J. M. Jaynes, and T. E. Cleveland. 2005. Disease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants. Plant Biotechnol. J. 3: 545-554. (Pubitemid 44944494)
30. Reddy, K. V., R. D. Yedery, and C. Aranha. 2004. Antimicrobial peptides: Premises and promises. Int. J. Antimicrob. Agents 24: 536-547.
31. Sundin, G. W. and C. L. Bender. 1993. Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 59: 1018-1024.
32. Vidaver, A. K. 2002. Uses of antimicrobials in plant agriculture. Clin. Infect. Dis. 34 Suppl 3: S107-S110.
33. Wan, Y. K., S. P. Tian, and G. Z. Qin. 2003. Enhancement of biocontrol activity of yeasts by adding sodium bicarbonate or ammonium molybdate to control postharvest disease of jujube fruits. Lett. Appl. Microbiol. 37: 249-253.
34. Yeaman, M. R. and N. Y. Yount. 2003. Mechanisms of antimicrobial peptide action and resistance. Pharmacol. Rev. 55: 27-55.
35. Yedery, R. D. and K. V. Reddy. 2005. Antimicrobial peptides as microbicidal contraceptives: Prophecies for prophylactics - a mini review. Eur. J. Contracept. Reprod. Health Care 10: 32-42.
36. Zasloff, M. 2002. Antimicrobial peptides of multicellular organisms. Nature 415: 389-395.
37. Zhao, C., T. Nguyen, L. M. Boo, T. Hong, C. Espiritu, D. Orlov, W. Wang, A. Waring, and R. I. Lehrer. 2001. RL-37, an alpha-helical antimicrobial peptide of the rhesus monkey. Antimicrob. Agents Chemother. 45: 2695-2702.