Application of antibodies against nonstructural protein 2B of dengue serotype 2 virus induced by DNA immunisation or recombinant protein NS 2B immunisation in BALB/c mice

Journal of Virological Methods

Volumn 163, Issue 1, 2010, Pages 10-16

  Liu, Limei ^a   Tian, Yanping ^a   Gao, Na ^a   Chen, Zong tao ^a   Zhang, Heng ^a   An, Jing ^b  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

Dengue virus; DNA immunisation; NS2B protein; Polyclonal antibody; Protein immunisation

Indexed keywords

NONSTRUCTURAL PROTEIN 2; NONSTRUCTURAL PROTEIN 2B; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS DNA;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY TITER; ARTICLE; BAGG ALBINO MOUSE; BLOOD SAMPLING; CONTROLLED STUDY; DENGUE VIRUS; DNA IMMUNIZATION; EUKARYOTIC CELL; EXPRESSION VECTOR; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENE AMPLIFICATION; GENE INSERTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SEROTYPE; VIRUS PATHOGENESIS; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CERCOPITHECUS AETHIOPS; DENGUE; DENGUE VIRUS; FEMALE; GENETIC VECTORS; IMMUNIZATION; MICE; MICE, INBRED BALB C; RECOMBINANT PROTEINS; VERO CELLS; VIRAL NONSTRUCTURAL PROTEINS;

DENGUE VIRUS; ESCHERICHIA COLI; EUKARYOTA; MUS; PROKARYOTA;

EID: 70649096916     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.04.011     Document Type: Article

References (22)

1. Barrett A.D. Japanese encephalitis and dengue vaccines. Biologicals 25 (1997) 7-34
2. Chambers T.J., Weir R.C., Grakoui A., McCourt D.W., Bazan J.F., Fletterick R.J., and Rice C.M. Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proc. Natl. Acad. Sci. U.S.A. 87 (1990) 8898-8902
3. Chambers T.J., Grakoui A., and Rice C.M. Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J. Virol. 65 (1991) 6042-6050
4. Chang Y.S., Liao C.L., Tsao C.H., Chen M.C., Liu C.I., Chen L.K., and Lin Y.L. Membrane permeabilization by small hydrophobic nonstructural proteins of Japanese encephalitis virus. J. Virol. 73 (1999) 6257-6264
5. Chen Z., Sahashi Y., Matsuo K., Asanuma H., Takahashi H., Iwasaki T., Suzuki Y., Aizawat C., Kurata T., and Tamura S.I. Comparison of the ability of viral protein-expressing plasmid DNAs to protect against influenza. Vaccine 16 (1998) 1544-1549
6. Falgout B., Pethel M., Zhang Y.M., and Lai C.J. Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J. Virol. 65 (1991) 2467-2475
7. Falgout B., Miller R.H., and Lai C.J. Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity. J. Virol. 67 (1993) 2034-2042
8. Gagnon S.J., Ennis F.A., and Rothman A.L. Bystander target cell lysis and cytokine production by dengue virus-specific human CD4+ cytotoxic T lymphocyte clones. J. Virol. 73 (1999) 3623-3629
9. Gardsvoll H., Solberg H., Dano K., and Hoyer-Hansen G. Generation of high-affinity rabbit polyclonal antibodies to the murine urokinase receptor using DNA immunization. J. Immunol. Methods 234 (2000) 107-116
10. Halstead S.B., and Deen J. The future of dengue vaccines. Lancet 360 (2002) 1243-1245
11. Kurane I., Brinton M.A., Samson A.L., and Ennis F.A. Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones. J. Virol. 65 (1991) 1823-1828
12. Mathew A., and Rothman A.L. Understanding the contribution of cellular immunity to dengue disease pathogenesis. Immunol. Rev. 225 (2008) 300-313
13. Roehrig J.T., Bolin R.A., and Kelly R.G. Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica. Virology 246 (1998) 317-328
14. Sampath A., and Padmanabhan R. Molecular targets for flavivirus drug discovery. Antivir. Res. 81 (2009) 6-15
15. Stephen C., Kurt E.E., and Padmanabhan R. Cotranslational membrane insertion of the serine proteinase precursor NS2B-NS3(pro) of dengue virus type 2 is required for efficient in vitro processing and is mediated through the hydrophobic regions of NS2B. J. Biol. Chem. 272 (1997) 30715-30723
16. Tang D.C., De Vit M., and Johnston S.A. Genetic immunization is a simple method for eliciting an immune response. Nature 356 (1992) 152-154
17. Tymciu S., Wijkhuisen A., Sagot M.A., Frobert Y., Grassi J., Créminon C., Couraud J.Y., and Boquet D. Use of DNA immunization to produce polyclonal antibodies against the natural human neurokinin-1 receptor for substance P. J. Neuroimmunol. 128 (2002) 23-30
18. Tian Y.P., Zhang J.L., Chen Z.T., Peng T., Xu X.F., Jiang W., and An J. Expression, purification of DENV-2 nonstructural protein 3 (NS3) and production of the polyclonal antibody for NS3. Dengue Bull. 30 (2006) 146-156
19. World Health Organization. Dengue Haemorrhagic Fever (1997), WHO Publications, Geneva
20. Yeung S.C., Anderson J., Kobayashi K., Oka K., and Chan L. Production of rabbit polyclonal antibody against apobec-1 by genetic immunization. J. Lipid Res. 38 (1997) 2627-2632
21. Young P.R., Hilditch P.A., Bletchly C., and Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J. Clin. Microbiol. 38 (2000) 1053-1057
22. Zuo Z., Liew O.W., Chen G., Chong P.C., Lee S.H., Chen K., Jiang H., Puah C.M., and Zhu W. Mechanism of NS2B-mediated activation of NS3pro in dengue virus: molecular dynamics simulations and bioassays. J. Virol. 83 (2009) 1060-1070