Expression, purification of DENV-2 nonstructural protein 3 (NS3) and production of the polyclonal antibody for NS3

Dengue Bulletin

Volumn 30, Issue , 2006, Pages 146-156

  Tian, Yanping ^a   Zhang, Junlei ^a   Chen, Zongtao ^a   Peng, Tao ^a   Xu, Xiaofeng ^a   Jiang, Wen ^a   An, Jing ^b  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

Author keywords

Dengue virus; Nonstructural protein 3; Polyclonal antibody

Indexed keywords

ANTISERUM; HYBRID PROTEIN; NONSTRUCTURAL PROTEIN 3; POLYCLONAL ANTIBODY;

AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DENGUE VIRUS; DENGUE VIRUS 2; DIAGNOSTIC VALUE; ESCHERICHIA COLI; IMMUNOFLUORESCENCE; IMMUNOGENICITY; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN TARGETING; RABBIT;

DENGUE VIRUS; DENGUE VIRUS TYPE 2; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 34848896955     PISSN: 1020895X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Leyssen P, De Clercq E, Neyts J. Perspectives for the treatment of infections with Flaviviridae. Clin Microbiol Rev 2000 Jan;13(1):67-82.
2. Irie K, Mohan PM, Sasaguri Y, Putnak R, Padmanabhan R. Sequence analysis of cloned dengue virus type 2 genome (New Guinea-C strain). Gene 1989 Feb 20;75(2):197-211.
3. Chambers TJ, Hahn CS, Galler R, Rice CM. Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 1990,44:649-88.
4. Chambers TJ, Weir RC, Grakoui A, McCourt DW, Bazan JF, Fletterick RJ, Rice CM. Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proc Natl Acad Sci U S A 1990 Nov;87(22):8898-902.
5. Falgout B, Miller RH, Lai CJ. Deletion analysis of dengue virus type 4 nonstructural protein NS2B: Identification of a domain required for NS2B-NS3 protease activity. J Virol 1993 Apr;67(4):2034-42.
6. Raviprakash K, Sinha M, Hayes CG, Porter KR. Conversion of dengue virus replicative form RNA (RF) to replicative intermediate (RI) by nonstructural proteins NS-5 and NS-3. Am J Trop Med Hyg 1998 Jan;58(1):90-5.
7. Utama A, Shimizu H, Morikawa S, Hasebe F, Morita K, Igarashi A, Hatsu M, Takamizawa K, Miyamura T. Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein. FEBS Lett 2000 Jan 7;465(1):74-8.
8. Wengler G, Wengler G. The NS 3 nonstructural protein of flaviviruses contains an RNA triphosphatase activity. Virology 1993 Nov;197(1):265-73.
9. Gwack Y, Kim DW, Han JH, Choe J. Characterization of RNA binding activity and RNA helicase activity of the hepatitis C virus NS3 protein. Biochem Biophys Res Commun 1996 Aug 14;225(2):654-9.
10. Warrener P, Collett MS. Pestivirus NS3 (p80) protein possesses RNA helicase activity. J Virol 1995 Mar;69(3):1720-6.
11. Kurane I, Zeng L, Brinton MA, Ennis FA. Definition of an epitope on NS3 recognized by human CD4+ cytotoxic T lymphocyte clones cross-reactive for dengue virus types 2, 3, and 4. Virology 1998 Jan 20;240(2):169-74.
12. Spaulding AC, Kurane I, Ennis FA, Rothman AL. Analysis of murine CD8(+) T-cell clones specific for the Dengue virus NS3 protein: flavivirus cross-reactivity and influence of infecting serotype. J Virol 1999 Jan; 73(1):398-403.
13. Tan CH, Yap EH, Singh M, Deubel V, Chan YC. Passive protection studies in mice with monoclonal antibodies directed against the non-structural protein NS3 of dengue 1 virus. J Gen Virol 1990 Mar;71 (Pt 3):745-9.
14. Valdes K, Alvarez M, Pupo M, Vazquez S, Rodriguez R, Guzman MG. Human dengue antibodies against structural and nonstructural proteins. Clin Diagn Lab Immunol 2000 Sep;7(5):856-7.
15. Rao XC, Li S, Hu JC, Jin XL, Hu XM, Huang JJ, Chen ZJ, Zhu JM, Hu FQ. A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli Protein Expr Purif 2004 Jul;36(1):11-8.
16. Rao X, Hu J, Li S, Jin X, Zhang C, Cong Y, Hu X, Tan Y, Huang J, Chen Z, Zhu J, Hu F. Design and expression of peptide antibiotic hPAB-beta as tandem multimers in Escherichia coli. Peptides 2005 May;26(5):721-9.
17. Villaverde A, Carrio MM. Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol Lett 2003 Sep;25(17):1385-95.
18. Arakaki TL, Fang NX, Fairlie DP, Young PR, Martin JL. Catalytically active Dengue virus NS3 protease forms aggregates that are separable by size exclusion chromatography. Protein Expr Purif 2002 Jul;25(2):241-7.