Efficient expression and secretion of recombinant human growth hormone in the methylotrophic yeast Pichia pastoris: Potential applications for other proteins

Biotechnology and Applied Biochemistry

Volumn 54, Issue 4, 2009, Pages 197-205

  Apte Deshpande, Anjali ^a   Rewanwar, Sachin ^a   Kotwal, Prakash ^a   Raiker, Veena A ^a   Padmanabhan, Sriram ^a  

^a LUPIN LIMITED   (India)

Author keywords

Agilent 2100 Bioanalyzer; Human growth hormone (hGH); Methanol induction; Oxygen transfer; Pichia pastoris; Surfactant

Indexed keywords

AGILENT; HUMAN GROWTH HORMONE; HUMAN GROWTH HORMONE (HGH); OXYGEN TRANSFER; PICHIA PASTORIS;

DESORPTION; METHANOL; OXYGEN; PROTEINS; SURFACE ACTIVE AGENTS;

CHEMICAL ANALYSIS;

ALCOHOL OXIDASE; HUMAN GROWTH HORMONE; METHANOL; POLYSORBATE 20; POLYSORBATE 80;

AMINO TERMINAL SEQUENCE; ARTICLE; BATCH REACTOR; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; FERMENTATION; GENE EXPRESSION SYSTEM; IMMUNOREACTIVITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN SECRETION; WESTERN BLOTTING;

BIOREACTORS; CLONING, MOLECULAR; FERMENTATION; GENE DOSAGE; GENE EXPRESSION; GENETIC VECTORS; HUMAN GROWTH HORMONE; HUMANS; PICHIA; POLYSORBATES;

PICHIA; PICHIA PASTORIS;

EID: 70649089334     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20090179     Document Type: Article

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