Enhanced secretion of human granulocyte colony-stimulating factor directed by a novel hybrid fusion peptide from recombinant Saccharomyces cerevisiae at high cell concentration

Biotechnology and Bioengineering

Volumn 57, Issue 5, 1998, Pages 600-609

  Bae, Cheon Soon ^a   Yang, Doo Suk ^a   Chang, Ki Ryong ^a   Seong, Baik Lin ^a   Lee, Jeewon ^a  

^a Hanhyo Institute of Technology   (South Korea)

Author keywords

Conformation; Fusion protein; Glycosylation; Multimer; RhG CSF; Secretion efficiency

Indexed keywords

AMINO ACIDS; BATCH CELL CULTURE; BIOSYNTHESIS; CELL MEMBRANES; COMPOSITION EFFECTS; CONFORMATIONS; PROTEINS; SURFACE ACTIVE AGENTS; YEAST;

GLYCOSYLATION; HUMAN GRANULOCYTE COLONY STIMULATING FACTOR;

GENETIC ENGINEERING;

GALACTOSE; GRANULOCYTE COLONY STIMULATING FACTOR; HYBRID PROTEIN; INTERLEUKIN 1BETA; POLYSORBATE 80; SIGNAL PEPTIDE;

ARTICLE; CIRCULAR DICHROISM; KLUYVEROMYCES; NONHUMAN; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; SACCHAROMYCES CEREVISIAE;

BIOTECHNOLOGY; CELL DIVISION; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GLYCOSYLATION; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; INTERLEUKIN-1; PEPTIDES; PLASMIDS; POLYSORBATES; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SURFACE-ACTIVE AGENTS;

EID: 0344605531     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980305)57:5<600::AID-BIT12>3.0.CO;2-F     Document Type: Article

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