Precision of protein aggregation measurements by sedimentation velocity analytical ultracentrifugation in biopharmaceutical applications

Analytical Biochemistry

Volumn 396, Issue 2, 2010, Pages 231-241

  Gabrielson, John P ^a   Arthur, Kelly K ^a   Stoner, Michael R ^b   Winn, Bradley C ^a   Kendrick, Brent S ^a   Razinkov, Vladimir ^c   Svitel, Juraj ^a   Jiang, Yijia ^a   Voelker, Paul J ^d   Fernandes, Cledwyn A ^d   Ridgeway, Ron ^e  

^a AMGEN INC   (United States)

^b GENENCOR INTERNATIONAL INC   (United States)

^c AMGEN INC   (United States)

^d Beckman Coulter Inc   (United States)

^e BECKMAN COULTER INC   (United States)

Author keywords

Analytical ultracentrifugation; Formulation; Protein aggregation; Sedimentation velocity

Indexed keywords

ASSOCIATION REACTIONS; CENTRIFUGATION; COLUMN CHROMATOGRAPHY; QUALITY CONTROL; SEDIMENTATION; SIZE EXCLUSION CHROMATOGRAPHY;

ANALYTICAL ULTRACENTRIFUGATION; BIOPHARMACEUTICAL DEVELOPMENT; EXPERIMENTAL APPROACHES; FORMULATION; INTERACTION EFFECT; MEASUREMENT PRECISION; PROTEIN AGGREGATION; SEDIMENTATION VELOCITIES;

PROTEINS;

ACETIC ACID; BOVINE SERUM ALBUMIN; CYTOKINE; HISTIDINE; IMMUNOGLOBULIN FC FRAGMENT; MANNITOL; MONOCLONAL ANTIBODY; POLYSORBATE; SODIUM CHLORIDE; SODIUM DIHYDROGEN PHOSPHATE; SORBITOL; SUCROSE;

ACCURACY; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DATA ANALYSIS; DRUG RESEARCH; GEL PERMEATION CHROMATOGRAPHY; INTERMETHOD COMPARISON; MATHEMATICAL ANALYSIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; QUALITY CONTROL; SEDIMENTATION RATE; ULTRACENTRIFUGATION;

EID: 70450240816     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.09.036     Document Type: Article

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