Information content analysis in automated microscopy imaging using an adaptive autofocus algorithm for multimodal functions

Journal of Microscopy

Volumn 236, Issue 3, 2009, Pages 194-202

  Brazdilova S L ^a   Kozubek, M ^a  

^a MASARYK UNIVERSITY   (Czech Republic)

Author keywords

Autofocus algorithm; Automated microscopy; Content function; Information content analysis; Multimodal focus function; Normalized variance

Indexed keywords

FLUORESCENCE MICROSCOPY; IMAGE ACQUISITION; INFORMATION USE;

AUTO-FOCUS ALGORITHM; AUTOMATED MICROSCOPY; CONTENT FUNCTION; FOCUS FUNCTION; INFORMATION CONTENT ANALYSIS; INFORMATION CONTENTS; LOCAL MAXIMUM; MULTI-MODAL; MULTIMODAL FOCUS FUNCTION; NORMALIZED VARIANCE;

AUTOMATION;

ALGORITHM; ARTICLE; AUTOMATION; CLUSTER ANALYSIS; CONTENT ANALYSIS; FLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; RELIABILITY; THEORETICAL STUDY;

EID: 70450207190     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03280.x     Document Type: Article

References (12)

1. Boddeke, F.R., van Vliet, L.J., Netten, H. Young, I.T. (1994) Auto-focusing in microscopy based on the OTF and sampling. Bioimaging 2, 193 203.
2. Brázdilová, S.L. (2007) Towards reliable autofocusing in automated microscopy. Proceedings of the Fourth International Conference on Informatics in Control, Automation and Robotics, Intelligent Control Systems and Optimization, pp. 440 447. INSTICC Press, Angers.
3. Bueno-Ibarra, M.A., Álvarez Borrego, J., Acho, L. Chávez-Sánchez, M.C. (2005) Fast autofocus algorithm for automated microscopes. Opt. Eng. Bellingham 44, 063601.1 063601.8.
4. Della Mea, V., Viel, F. Beltrami, C.A. (2005) A pixel-based autofocusing technique for digital histologic and cytologic slides. Comput. Med. Imaging Graph. 29 (5 333 341.
5. Firestone, L., Cook, K., Culp, K., Talsania, N. Preston, K. (1991) Comparison of autofocus methods for automated microscopy. Cytometry 12, 195 206.
6. Groen, F.C., Young, I.T. Ligthart, G. (1985) A comparison of different focus functions for use in autofocus algorithms. Cytometry 6, 81 91.
7. Hilsenstein, V. (2005) Robust autofocusing for automated microscopy imaging of fluorescently labelled bacteria. Digital Image Computing: Techniques and Applications, IEEE Computer Society, Washington.
8. Kozubek, M., Kozubek, S., Lukášová, E., Marečková, A., Bártová, E., Skalníková , M. Jergová, A. (1999) High-resolution cytometry of fish dots in interphase cell nuclei. Cytometry 36, 279 293.
9. Netten, H. (1997) Automated image analysis of FISH-stained cell nuclei. PhD Thesis, Delft University of Technology, Delft.
10. Pech-Pacheco, J.L., Cristóbal, G., Chamorro-Martínez, J. Fernández-Valdivia, J. (2000) Diatom autofocusing in brightfield microscopy: a comparative study. Proceedings of the International Conference on Pattern Recognition (ICPR'00), IEEE Computer Society, Washington.
11. Santos, A., Ortiz de Solórzano, C., Vaquero, J.J., Peña, J.M., Malpica, N. del Pozo, F. (1997) Evaluation of autofocus functions in molecular cytogenetic analysis. J. Microsc. 188, 264 272.
12. Sun, Y., Duthaler, S. Nelson, B.J. (2004) Autofocusing in computer microscopy: selecting the optimal focus algorithm. Microsc. Res. Tech. 65, 139 149.