Expression of human Cu, Zn-superoxide dismutase in an insect cell-free system and its structural analysis by MALDI-TOF MS

Journal of Biotechnology

Volumn 144, Issue 4, 2009, Pages 287-292

  Ezure, Toru ^a   Suzuki, Takashi ^a   Ando, Eiji ^a   Nishimura, Osamu ^b   Tsunasawa, Susumu ^b  

^a Clinical and Biotechnology Business Unit   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Cell free protein synthesis system; hSOD1; Insect cell; MALDI TOF MS; Metalloprotein

Indexed keywords

CELL FREE PROTEIN SYNTHESIS; CU ,ZN SUPEROXIDE DISMUTASE; CYTOSOLS; DENATURING CONDITIONS; DISULFIDE BONDS; ENZYMATIC ACTIVITIES; HOMODIMERS; HUMAN ERYTHROCYTES; INSECT CELLS; MALDI TOF MS; METALLOPROTEIN; REACTION VOLUME; REDUCING ENVIRONMENT; SPODOPTERA FRUGIPERDA; STRUCTURAL STABILITIES; ZINC IONS;

MASS SPECTROMETRY; METAL IONS; METAL REFINING; OXYGEN; PROTEINS; STABILITY; STRUCTURAL ANALYSIS;

ZINC;

COPPER ION; COPPER ZINC SUPEROXIDE DISMUTASE; RECOMBINANT ENZYME; ZINC ION;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; DISULFIDE BOND; ENZYME ANALYSIS; ENZYME STRUCTURE; ERYTHROCYTE; INSECT CELL; LEPIDOPTERA; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; BIOTECHNOLOGY; CELL-FREE SYSTEM; HUMANS; MOLECULAR SEQUENCE DATA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPODOPTERA; STRUCTURE-ACTIVITY RELATIONSHIP; SUPEROXIDE DISMUTASE;

HEXAPODA; SPODOPTERA FRUGIPERDA;

EID: 70449720539     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.09.017     Document Type: Article

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