Dichloran rose-bengal chloramphenicol agar: Preferred medium for isolating mycotoxigenic fungal contaminants in silage

World Mycotoxin Journal

Volumn 2, Issue 4, 2009, Pages 419-427

  Van Pamel, E ^a   Daeseleire, E ^a   De Boever, J ^a   Heyndrickx, M ^a   Herman, L ^a   Verbeken, A ^b   Vlaemynck, G ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Aspergillus; Fusarium; Maize silage; Mycotoxigenic moulds; Penicillium

Indexed keywords

AGAR; CHLORAMPHENICOL; MYCOPHENOLIC ACID; MYCOTOXIN; PATULIN; ROQUEFORTIN C; ROSE BENGAL; UNCLASSIFIED DRUG;

AGAR MEDIUM; ANIMAL HEALTH; ARTICLE; ASPERGILLUS FUMIGATUS; BACTERIAL COUNT; BEET; BELGIUM; CULTURE MEDIUM; FERMENTATION; FUNGAL CONTAMINATION; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; MAIZE; MICROSCOPY; MOULD; NONHUMAN; PENICILLIUM; PENICILLIUM PANEUM; PENICILLIUM ROQUEFORTI; RESEARCH; RISK; SILAGE;

ANIMALIA; ASPERGILLUS; ASPERGILLUS FUMIGATUS; BETA VULGARIS SUBSP. VULGARIS; FUNGI; FUSARIUM; PENICILLIUM; PENICILLIUM PANEUM; PENICILLIUM ROQUEFORTII; ZEA MAYS;

EID: 70449627534     PISSN: 18750710     EISSN: 18750796     Source Type: Journal    
DOI: 10.3920/WMJ2009.1143     Document Type: Article

References (36)

1. Aucherbach, H., Oldenburg, E. and Weissbach, F., 1998. Incidence of Penicillium roqueforti and roquefortine C in silages. Journal of the Science of Food and Agriculture 76: 565-572.
2. Beckers, H.J., De Boer, E., Dijkmann, K.E., Hartog, B.J., Van Kooij, J.A., Kuik, D., Mol, N., Nooitgedagt, A.J., Northolt, M.D. and Samson, R.A., 1986. Comparison of various media for the enumeration of yeasts and moulds in food. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L. (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 66-67.
3. Bennet, J.W. and Klich, M., 2003. Mycotoxins. Clinical Microbiology Reviews 16: 497-516.
4. Boysen, M.E., Jacobsson, K.G. and Schnurer, J., 2000. Molecular identification of species from the Penicillium roqueforti group associated with spoiled animal feed. Applied and Environmental Microbiology 66: 1523-1526.
5. Byrem, T.M., Pestka, J.J., Chu, F.S. and Strasburg, G.M., 1999. Analysis and pharmacokinetics of cyclopiazonic acid in market weight pigs. Journal of Animal Science 77: 173-179.
6. Cole, R.J., Kirksey, J.W., Dorned, J.W., Wilson, D.M., Johnson, J., Jr., Bedell, D., Springer, J.P., Chexal, K.K., Clardy, J. and Cox, R.H., 1977. Mycotoxins produced by Aspergillus fumigatus isolated from silage. Annales de la Nutrition et d'Alimentation 31: 685-691.
7. Deák, T., Török, T., Lehoczki, J., Reichart, O., Tabajdi-Pinter, V. and Fabri, I., 1986. Comparison of general media for the enumeration of molds and yeasts. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 114-119.
8. European Commission (EC), 1971. Second Commission Directive 71/393/EEC of 18 November 1971 establishing Community methods of analysis for the official control of feedingstuffs. Official Journal of the European Union L 279: 7-18.
9. Fink-Gremmels, J., 2008. Mycotoxins in cattle feeds and carry-over to dairy milk: a review. Food Additives and Contaminants 25: 172-180.
10. Garon, D., Richard, E., Sage, L., Bouchart, V., Pottier, D. and Lebailly, P., 2006. Mycoflora and multimycotoxin detection in corn silage: experimental study. Journal of Agricultural and Food Chemistry 54: 3479-3484.
11. Gawehn, K., 1984. D(-)-Lactate. In: Bergmeyer, H.U., Bergmeyer, J. and Grals, M. (eds.) Methods of enzymatic analysis. Volume VI. Verlag Chemie, Weinheim, Germany, pp. 588-592.
12. Golosov, I.M., Boltushkin, A.N. and Kovalskaia, M.G., 1967. The viability of the genus Fusarium in silage. Veterinaria 44: 98-100.
13. Hastings, J.W., Tsai, W.Y.J. and Bullerman, L.B., 1986. Comparison of a general purpose mycological medium with a selective mycological medium for mold enumeration. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 94-96.
14. International Organization for Standardization (ISO), 2005. ISO 5983- 2. Animal feeding stuffs - determination of nitrogen content and calculation of crude protein content - part 2: block digestion/steam distillation method, ISO, Geneva, Switzerland, pp. 14.
15. Jouany, J.P., 1981. Dosages des acides gras volatils et des alcools dans les ensilages par chromatographie en phase gazeuse. Bulletin Technique Centre de Recherches Zootechniques et Vétérinaires de Theix (INRA) 46: 63-66.
16. Kellen, L.A., Smith, T.M., Hannon, C.B. and Olson, K.E., 1986. Comparative study of media for the detection and enumeration of molds and yeasts. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 103-106.
17. King, A.D., Hocking, A.D. and Pitt, J.I., 1979. Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Applied and Environmental Microbiology 37: 959-964.
18. Kung, L. and Stokes, M.R., 2002. Analysing silages for fermentation end products. Available at: http://ag.udel.edu/anfs/faculty/kung/articles/ analyzing-silages-for-fermentati.htm. Accessed 7 August 2008.
19. Lenovich, L.M., Walters, J.L. and Reed, D.M., 1986. Comparison of media for the enumeration of fungi from dried foods. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp.76-83.
20. Mancini, N., Ossi, C.M., Perotti, M., Carletti, S., Gianni, C., Paganoni, G., Matuska, S., Guglielminetti, M., Cavallero, A., Burioni, R., Rama, P. and Clementi, M., 2005. Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. Journal of Medical Microbiology 54: 897-900.
21. Mansfield, M.A. and Kuldau, G.A., 2007. Microbiological and molecular determination of mycobiota in fresh and ensiled maize silage. Mycologia 99: 269-278.
22. Norred, W.P., Porter, J.K., Dorner, J.W. and Cole, R.J., 1998. Occurrence of the mycotoxin cyclopiazonic acid in meat after oral administration to chickens. Journal of Agricultural and Food Chemistry 36: 113-116.
23. Nout, M.J.R., Bouwmeester, H.M., Haaksma, J. and Van Dijk, H., 1993. Fungal growth in silages of sugarbeet and maize. Journal of the Science of Food and Agriculture 11: 458-466.
24. O'Brien, M., Nielsen, K.F., O'Kiely, P., Forristal, P.D., Fuller, H.T. and Frisvad, J.C., 2006. Mycotoxins and other secondary metabolites produced in vitro by Penicillium paneum Frisvad and Penicillium roqueforti Thom isolated from baled grass silage in Ireland. Journal of Agricultural and Food Chemistry 54: 9268-9276.
25. Pelhate, J., 1977. Maize silage: incidence of moulds during conservation. Folia Veterinaria Latina 7: 1-16.
26. Pitt, J.I., 1986. Properties of the ideal fungal enumeration medium. In King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L. (eds.) Methods for the mycological examination of food. Plenum Press, New York, USA, pp. 63-65.
27. Reutter, M., 1999. Zearalenon und Deoxynivalenol in Getreide und Futtermitteln Schleswig-Holsteins: Untersuchungen aus dem Erntejahr 1998. Mycotoxin Research 15/A: 5-9.
28. Richard, E., Heutte, N., Sage, L., Pottier, D., Bouchart, V., Lebailly, P. and Garon, D., 2007. Toxigenic fungi and mycotoxins in mature corn silage. Food and Chemical Toxicology 45: 2420-2425.
29. Rochard-Molard, D., 1986. Comparison of five media for the enumeration of molds in cereals and cereal products. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 109-111.
30. Samson, R.A., Hoekstra, E.S. and Frisvad, J.C., 2004. Introduction of food- and airborne fungi. Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands, 389 p.
31. Schneweis, I., Meyer, K., Hörmansdorfer, S. and Bauer, J., 2000. Mycophenolic acid in silage. Applied and Environmental Microbiology 266: 3639-3641.
32. Scudamore, K.A. and Livesey, C.T., 1998. Occurrence and significance of mycotoxins in forage crops and silage: a review. Journal of the Science of Food and Agriculture 77: 1-17.
33. Seiler, D.A.L., 1986. A collaborative exercise comparing media for enumerating fungi in foods. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 83-85.
34. Skaar, I. and Stenwig, H., 1996. Malt-yeast extract sucrose agar, a suitable medium for enumeration and isolation of fungi from silage. Applied and Environmental Microbiology 62: 3614-3619.
35. White, T., Burns, T., Lee, S. and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J. (eds.) PCR protocols. Academic Press, San Diego, CA, USA, pp. 315-322.
36. Williams, A.P., 1986. A comparison of DRBC, RBC an MEA media for the enumeration of molds and yeasts in pure culture and in foods. In: King, A.D., Pitt, J.I., Beuchat, L.R. and Corry, J.E.L (eds.) Methods for the mycological examination of food. Plenum Press, New York, NY, USA, pp. 85-89.