Silencing of GRP94 expression promotes apoptosis in pancreatic cancer cells

International Journal of Oncology

Volumn 35, Issue 4, 2009, Pages 823-828

  Pan, Zheng ^a   Erkan, Mert ^a   Streit, Sylvia ^a   Friess, Helmut ^a   Kleeff, Jörg ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Apoptosis; Capheron; Chemotherapy; Pancreatic cancer; TRA1

Indexed keywords

DACTINOMYCIN; GLUCOSE REGULATED PROTEIN 94; MESSENGER RNA; SMALL INTERFERING RNA;

ACINAR CELL; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CANCER INHIBITION; CANCER SURVIVAL; CHRONIC PANCREATITIS; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION REGULATION; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; PANCREAS CARCINOMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

APOPTOSIS; CARCINOMA, PANCREATIC DUCTAL; CELL LINE, TUMOR; DACTINOMYCIN; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOHISTOCHEMISTRY; KAPLAN-MEIERS ESTIMATE; MEMBRANE GLYCOPROTEINS; NEOPLASM STAGING; NUCLEIC ACID SYNTHESIS INHIBITORS; PANCREATIC NEOPLASMS; PROGNOSIS; PROTEIN SYNTHESIS INHIBITORS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; TIME FACTORS; TRANSFECTION; TUMOR MARKERS, BIOLOGICAL;

EID: 70449408715     PISSN: 10196439     EISSN: 17912423     Source Type: Journal    
DOI: 10.3892/ijo_00000395     Document Type: Article

References (34)

1. Van Cutsem E, Aerts R, Haustermans K, Topal B, Van Steenbergen W and Verslype C: Systemic treatment of pancreatic cancer. Eur J Gastroenterol Hepatol 16: 265-274, 2004.
2. Erkan M, Kleeff J, Gorbachevski A, et al: Periostin creates a tumor-supportive microenvironment in the pancreas by sustaining fibrogenic stellate cell activity. Gastroenterology 132: 1447-1464, 2007. (Pubitemid 46627568)
3. Erkan M, Michalski CW, Rieder S, et al: The activated stroma index is a novel and independent prognostic marker in pancreatic ductal adenocarcinoma. Clin Gastroenterol Hepatol 6: 1155-1161, 2008.
4. Michalski CW, Kleeff J, Bachmann J, et al: Second-look operation for unresectable pancreatic ductal adenocarcinoma at a high-volume center. Ann Surg Oncol 15: 186-192, 2008.
5. Erkan M, Kleeff J, Esposito I, et al: Loss of BNIP3 expression is a late event in pancreatic cancer contributing to chemoresistance and worsened prognosis. Oncogene 24: 4421-4432, 2005. (Pubitemid 40961765)
6. Michalski CW, Erkan M, Sauliunaite D, et al: Ex vivo chemo-sensitivity testing and gene expression profiling predict response towards adjuvant gemcitabine treatment in pancreatic cancer. Br J Cancer 99: 760-767, 2008.
7. Hanahan D and Weinberg RA: The hallmarks of cancer. Cell 100: 57-70, 2000.
8. Fu Y and Lee AS: Glucose regulated proteins in cancer progression, drug resistance and immunotherapy. Cancer Biol Ther 5: 741-744, 2006. (Pubitemid 44775126)
9. Koumenis C: ER stress, hypoxia tolerance and tumor progression. Curr Mol Med 6: 55-69, 2006.
10. Li J and Lee AS: Stress induction of GRP78/BiP and its role in cancer. Curr Mol Med 6: 45-54, 2006.
11. Nicchitta CV: Re-evaluating the role of heat-shock protein-peptide interactions in tumour immunity. Nat Rev Immunol 3: 427-432, 2003.
12. Srivastava PK: Therapeutic cancer vaccines. Curr Opin Immunol 18: 201-205, 2006.
13. Wang XY, Li Y, Yang G and Subjeck JR: Current ideas about applications of heat shock proteins in vaccine design and immunotherapy. Int J Hyperthermia 21: 717-722, 2005. (Pubitemid 41807098)
14. Melnick J, Dul JL and Argon Y: Sequential interaction of the chaperones BiP and GRP94 with immunoglobulin chains in the endoplasmic reticulum. Nature 370: 373-375, 1994. (Pubitemid 2110873)
15. Banerjea A, Ahmed S, Hands RE, et al: Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity. Mol Cancer 3: 21, 2004. (Pubitemid 40230551)
16. Kubota H, Suzuki T, Lu J, et al: Increased expression of GRP94 protein is associated with decreased sensitivity to X-rays in cervical cancer cell lines. Int J Radiat Biol 81: 701-709, 2005. (Pubitemid 43169761)
17. Lee AS: The glucose-regulated proteins: stress induction and clinical applications. Trends Biochem Sci 26: 504-510, 2001. (Pubitemid 32735452)
18. Reddy RK, Lu J and Lee AS: The endoplasmic reticulum chaperone glycoprotein GRP94 with Ca(2+)-binding and anti-apoptotic properties is a novel proteolytic target of calpain during etoposide-induced apoptosis. J Biol Chem 274: 28476-28483, 1999. (Pubitemid 129504716)
19. Bando Y, Katayama T, Aleshin AN, Manabe T and Tohyama M: GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis. Apoptosis 9: 501-508, 2004. (Pubitemid 38987587)
20. Lee SH, Song R, Lee MN, et al: A molecular chaperone glucose-regulated protein 94 blocks apoptosis induced by virus infection. Hepatology 47: 854-866, 2008.
21. Kleeff J, Kornmann M, Sawhney H and Korc M: Actinomycin D induces apoptosis and inhibits growth of pancreatic cancer cells. Int J Cancer 86: 399-407, 2000. (Pubitemid 30187502)
22. Reiser-Erkan C, Erkan M, Pan Z, et al: Hypoxia-inducible proto-oncogene Pim-1 is a prognostic marker in pancreatic ductal adenocarcinoma. Cancer Biol Ther 7: 1352-1359, 2008.
23. Gazit G, Lu J and Lee AS: De-regulation of GRP stress protein expression in human breast cancer cell lines. Breast Cancer Res Treat 54: 135-146, 1999. (Pubitemid 29295316)
24. Nomura H, Uzawa K, Yamano Y, et al: Network-based analysis of calcium-binding protein genes identifies Grp94 as a target in human oral carcinogenesis. Br J Cancer 97: 792-801, 2007. (Pubitemid 47396293)
25. Wang Q, He Z, Zhang J, et al: Overexpression of endoplasmic reticulum molecular chaperone GRP94 and GRP78 in human lung cancer tissues and its significance. Cancer Detect Prev 29: 544-551, 2005. (Pubitemid 41758494)
26. Zheng HC, Takahashi H, Li XH, et al: Overexpression of GRP78 and GRP94 are markers for aggressive behavior and poor prognosis in gastric carcinomas. Hum Pathol 39: 1042-1049, 2008.
27. Wang XP, Liu GZ, Song AL, Chen RF, Li HY and Liu Y: Expression and significance of heat shock protein 70 and glucose-regulated protein 94 in human esophageal carcinoma. World J Gastroenterol 11: 429-432, 2005. (Pubitemid 40125257)
28. Wang XP, Qiu FR, Liu GZ and Chen RF: Correlation between clinicopathology and expression of heat shock protein 70 and glucose-regulated protein 94 in human colonic adenocarcinoma. World J Gastroenterol 11: 1056-1059, 2005. (Pubitemid 40347764)
29. Misra UK, Deedwania R and Pizzo SV: Binding of activated alpha2-macroglobulin to its cell surface receptor GRP78 in 1-LN prostate cancer cells regulates PAK-2-dependent activation of LIMK. J Biol Chem 280: 26278-26286, 2005. (Pubitemid 41022225)
30. Misra UK, Deedwania R and Pizzo SV: Activation and cross-talk between Akt, NF-kappaB, and unfolded protein response signaling in 1-LN prostate cancer cells consequent to ligation of cell surface-associated GRP78. J Biol Chem 281: 13694-13707, 2006. (Pubitemid 43855285)
31. Bando Y, Katayama T, Kasai K, Taniguchi M, Tamatani M and Tohyama M: GRP94 (94 kDa glucose-regulated protein) suppresses ischemic neuronal cell death against ischemia/ reperfusion injury. Eur J Neurosci 18: 829-840, 2003. (Pubitemid 37059693)
32. Sheu ML, Liu SH and Lan KH: Honokiol induces calpain-mediated glucose-regulated protein-94 cleavage and apoptosis in human gastric cancer cells and reduces tumor growth. PLoS ONE 2: e1096, 2007.
33. Parmiani G, Testori A, Maio M, et al: Heat shock proteins and their use as anticancer vaccines. Clin Cancer Res 10: 8142-8146, 2004. (Pubitemid 40053371)
34. Wang XH, Qin Y, Hu MH and Xie Y: Dendritic cells pulsed with gp96-peptide complexes derived from human hepatocellular carcinoma (HCC) induce specific cytotoxic T lymphocytes. Cancer Immunol Immunother 54: 971-980, 2005. (Pubitemid 41187705)