Secretory expression of functional barley limit dextrinase by Pichia pastoris using high cell-density fermentation

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 112-119

  Vester Christensen, Malene Bech ^a   Hachem, Maher Abou ^a   Naested, Henrik ^a   Svensson, Birte ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Barley limit dextrinase; Cyclodextrin affinity; High cell density fermentation; Pichia pastoris; Pullulan hydrolysis

Indexed keywords

CYCLODEXTRIN; GLUCAN; GLYCOSIDASE; PULLULAN; PULLULANASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BARLEY; BIOREACTOR; BIOTECHNOLOGY; CELL FRACTIONATION; CHEMISTRY; ENZYMOLOGY; FERMENTATION; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; REGRESSION ANALYSIS; SECRETION; SURFACE PLASMON RESONANCE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOREACTORS; BIOTECHNOLOGY; CLONING, MOLECULAR; CYCLODEXTRINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FERMENTATION; GLUCANS; GLYCOSIDE HYDROLASES; HORDEUM; LEAST-SQUARES ANALYSIS; MOLECULAR SEQUENCE DATA; PICHIA; PLASMIDS; RECOMBINANT PROTEINS; SUBCELLULAR FRACTIONS; SURFACE PLASMON RESONANCE; TRANSFORMATION, GENETIC;

EMBRYOPHYTA; HORDEUM; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 70350776503     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.08.016     Document Type: Article

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