Production of enzymatically active recombinant full-length barley high pI α-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification

Protein Expression and Purification

Volumn 46, Issue 1, 2006, Pages 56-63

  Naested, Henrik ^a,b   Kramhøft, Birte ^a,b   Lok, Finn ^a   Bojsen, Kirsten ^a   Yu, Shukun ^c   Svensson, Birte ^a,b  

^a CARLSBERG LABORATORY   (Denmark)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c DANISCO A S   (Denmark)

Author keywords

Barley high pI glucosidase; High cell density fermentation; Maltose; Metal chelating affinity chromatography; Pichia pastoris

Indexed keywords

ALPHA GLUCOSIDASE; PRIMER DNA; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BARLEY; ENZYMOLOGY; FERMENTATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PHYSIOLOGY; PICHIA; PLASMID; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; ULTRAFILTRATION;

ALPHA-GLUCOSIDASES; CHROMATOGRAPHY, AFFINITY; DNA PRIMERS; FERMENTATION; HORDEUM; KINETICS; PICHIA; PLANT PROTEINS; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RESTRICTION MAPPING; ULTRAFILTRATION;

EMBRYOPHYTA; HORDEUM VULGARE SUBSP. VULGARE; PICHIA PASTORIS;

EID: 32644463519     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.10.008     Document Type: Article

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