Automated live microscopy to study mitotic gene function in fluorescent reporter cell lines

Methods in Molecular Biology

Volumn 545, Issue , 2009, Pages 113-134

  Schmitz, Michael H A ^a   Gerlich, Daniel W ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Automated live cell imaging; Green Fluorescent Protein (GFP); laser scanning confocal microscopy; mitosis; multidimensional microscopy; stably expressing reporter cell lines; widefield epifluorescence microscopy

Indexed keywords

CELL CYCLE PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; CELL COMPARTMENTALIZATION; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; GENETICS; HELA CELL; HUMAN; METABOLISM; METHODOLOGY; MITOSIS; REPORTER GENE;

CELL COMPARTMENTATION; CELL CYCLE PROTEINS; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOSIS; RECOMBINANT FUSION PROTEINS;

EID: 70350727266     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-993-2_7     Document Type: Article

References (27)

1. Gerlich, D., Beaudouin, J., Gebhard, M., Ellenberg, J., and Eils, R. (2001) Fourdimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells. Nat Cell Biol 3, 852-5. (Pubitemid 32842693)
2. Gerlich, D. and Ellenberg, J. (2003) 4D imaging to assay complex dynamics in live specimens. Nat Cell Biol Suppl, S14-9.
3. Dailey, M.E., Manders, E., Soll, D.R., and Terasaki, M. (2006) Confocal Microscopy of Living Cells, in Handbook of Biological Confocal Microscopy (Pawley, J.B., ed.), Springer, New York, NY, pp. 381-403.
4. Bodnar, A.G., Ouellette, M., Frolkis, M., Holt, S.E., Chiu, C.P., Morin, G.B., Harley, C.B., Shay, J.W., Lichtsteiner, S., and Wright, W.E. (1998) Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349-52. (Pubitemid 28063364)
5. Neumann, B., Held, M., Liebel, U., Erfle, H., Rogers, P., Pepperkok, R., and Ellenberg, J. (2006) High-throughput RNAi screening by time-lapse imaging of live human cells. Nat Methods 3, 385-90.
6. Kanda, T., Sullivan, K.F., and Wahl, G.M. (1998) Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 8, 377-85. (Pubitemid 28173996)
7. Snapp, E.L., Hegde, R.S., Francolini, M., Lombardo, F., Colombo, S., Pedrazzini, E., Borgese, N., and Lippincott-Schwartz, J. (2003) Formation of stacked ER cisternae by low affinity protein interactions. J Cell Biol 163, 257-69. (Pubitemid 37363127)
8. Shu, X., Shaner, N.C., Yarbrough, C.A., Tsien, R.Y., and Remington, S.J. (2006) Novel chromophores and buried charges control color in mFruits. Biochemistry 45, 9639-47. (Pubitemid 44257411)
9. Campbell, R.E., Tour, O., Palmer, A.E., Steinbach, P.A., Baird, G.S., Zacharias, D.A., and Tsien, R.Y. (2002) A monomeric red fluorescent protein. Proc Natl Acad Sci USA 99, 7877-82. (Pubitemid 34650973)
10. Stepanova, T., Slemmer, J., Hoogenraad, C.C., Lansbergen, G., Dortland, B., De Zeeuw, C.I., Grosveld, F., van Cappellen, G., Akhmanova, A., and Galjart, N. (2003) Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (endbinding protein 3-green fluorescent protein). J Neurosci 23, 2655-64. (Pubitemid 36418600)
11. Piel, M., Meyer, P., Khodjakov, A., Rieder, C.L., and Bornens, M. (2000) The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells. J Cell Biol 149, 317-30.
12. Sugimoto, K., Fukuda, R., and Himeno, M. (2000) Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. Cell Struct Funct 25, 253-61. (Pubitemid 32007609)
13. Zacharias, D.A., Violin, J.D., Newton, A.C., and Tsien, R.Y. (2002) Partitioning of lipidmodified monomeric GFPs into membrane microdomains of live cells. Science 296, 913-6. (Pubitemid 34464897)
14. Leonhardt, H., Rahn, H.P., Weinzierl, P., Sporbert, A., Cremer, T., Zink, D., and Cardoso, M.C. (2000) Dynamics of DNA replication factories in living cells. J Cell Biol 149, 271-80.
15. Zaal, K.J., Smith, C.L., Polishchuk, R.S., Altan, N., Cole, N.B., Ellenberg, J., Hirschberg, K., Presley, J.F., Roberts, T.H., Siggia, E., Phair, R.D., and Lippincott-Schwartz, J. (1999) Golgi membranes are absorbed into and reemerge from the ER during mitosis. Cell 99, 589-601. (Pubitemid 30004635)
16. Terasaki, M., Jaffe, L.A., Hunnicutt, G.R., and Hammer, J.A., 3rd (1996) Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein. Dev Biol 179, 320-8. (Pubitemid 26384077)
17. Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R.Y., and Pozzan, T., et al. (1996) Double labelling of subcellular structures with organelletargeted GFP mutants in vivo. Curr Biol 6, 183-8.
18. Muhlhausser, P. and Kutay, U. (2007) An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubuledependent steps in nuclear envelope breakdown. J Cell Biol 178, 595-610. (Pubitemid 47267272)
19. Rao, P.N. and Engelberg, J. (1965) Hela cells: Effects of temperature on the life cycle. Science 148, 1092-4.
20. Rieder, C.L. and Cole, R.W. (2002) Coldshock and the Mammalian cell cycle. Cell Cycle 1, 169-75.
21. Waters, J.C. (2007) Live-cell fluorescence imaging. Methods Cell Biol 81, 115-40.
22. Lippincott-Schwartz, J., Altan-Bonnet, N., and Patterson, G.H. (2003) Photobleaching and photoactivation: following protein dynamics in living cells. Nat Cell Biol Suppl, S7-14. (Pubitemid 37082167)
23. Swedlow, J.R. (2007) Quantitative fluorescence microscopy and image deconvolution. Methods Cell Biol 81, 447-65.
24. Abramowitz, M., Spring, K.R., Keller, H.E., and Davidson, M.W. (2002) Basic principles of microscope objectives. Biotechniques 33, 772-4, 776-8, 780-1.
25. Keller, H.E. (2006) Objective lenses for confocal microscopy, in Handbook of Biological Confocal Microscopy (Pawley, J.B., ed.), Springer, New York, pp. 145-161.
26. Inoúe, S. and Spring, K. (1986) Video Microscopy. Plenum Press, New York.
27. Rabut, G. and Ellenberg, J. (2004) Automatic real-time three-dimensional cell tracking by fluorescence microscopy. J Microsc 216, 131-7. (Pubitemid 39469786)