PCR-suppression effect: Kinetic analysis and application to representative or long-molecule biased PCR-based amplification of complex samples

Journal of Biotechnology

Volumn 128, Issue 3, 2007, Pages 435-443

  Dai, Zhong Min ^a   Zhu, Xiao Jing ^a   Chen, Qi ^a   Yang, Wei Jun ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Annealing temperature; Complex PCR; Full length cDNA; PCR suppression effect; Representation

Indexed keywords

ANNEALING; COMPLEXATION; DNA; MOLECULAR STRUCTURE; PARTICLE SIZE ANALYSIS; SYNTHESIS (CHEMICAL);

ANNEALING TEMPERATURE; COMPLEX PCR; FULL LENGTH CDNA; PCR-SUPPRESSION EFFECT; REPRESENTATION;

ENZYME KINETICS;

COMPLEMENTARY DNA;

ARTICLE; DNA SYNTHESIS; GENE AMPLIFICATION; KINETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DESIGN; TEMPERATURE;

BASE SEQUENCE; DNA, COMPLEMENTARY; DNA, SINGLE-STRANDED; KINETICS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; TEMPLATES, GENETIC;

EID: 33846536399     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.10.018     Document Type: Article

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