Administration of connexin43 siRNA abolishes secretory pulse synchronization in GnRH clonal cell populations

Molecular and Cellular Endocrinology

Volumn 314, Issue 1, 2010, Pages 75-83

  Bose, Sudeep ^a   Leclerc, Gilles M ^b   Vasquez Martinez, Rafael ^c   Boockfor, Fredric R ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b UNIVERSITY OF MIAMI   (United States)

^c UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

GT1 7 cells; Microinjection; Real time RT PCR; Rhythmicity; Single cell imaging; siRNA

Indexed keywords

CONNEXIN 26; CONNEXIN 43; GONADORELIN; MESSENGER RNA; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL CULTURE; CELL SYNCHRONIZATION; CONTROLLED STUDY; GAP JUNCTION; HYPOTHALAMUS; IMMUNOREACTIVITY; MICROINJECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PULSATILE HORMONE RELEASE;

ANIMALS; CELL LINE; CONNEXIN 43; CONNEXINS; GONADOTROPIN-RELEASING HORMONE; HYPOTHALAMUS; MALE; MICE; NEURONS; RNA, MESSENGER; RNA, SMALL INTERFERING;

EID: 70350553826     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mce.2009.08.016     Document Type: Article

References (53)

1. Bedner P., Niessen H., Odermatt B., Kretz M., Willecke K., and Harz H. Selective permeability of different connexin channels to the second messenger cyclic AMP. J. Biol. Chem. 281 (2006) 6673-6681
2. Bennett M.V., and Zukin R.S. Electrical coupling and neuronal synchronization in the mammalian brain. Neuron 41 (2004) 495-511
3. Bittman K.S., and LoTurco J.J. Differential regulation of connexin 26 and 43 in murine neocortical precursors. Cereb. Cortex 9 (1999) 188-195
4. Campbell R.E., Han S.K., and Herbison A.E. Biocytin filling of adult gonadotropin-releasing hormone neurons in situ reveals extensive, spiny, dendritic processes. Endocrinology 146 (2005) 1163-1169
5. Connors B.W., and Long M.A. Electrical synapses in the mammalian brain. Annu. Rev. Neurosci. 27 (2004) 393-418
6. Cottrell E.C., Campbell R.E., Han S.K., and Herbison A.E. Postnatal remodeling of dendritic structure and spine density in gonadotropin-releasing hormone neurons. Endocrinology 147 (2006) 3652-3661
7. Crowley Jr. W.F., and Jameson J.L. Clinical counterpoint: gonadotropin-releasing hormone deficiency: perspectives from clinical investigation. Endocr. Rev. 13 (1992) 635-640
8. Dermietzel R., Traub O., Hwang T.K., Beyer E., Bennett M.V., Spray D.C., and Willecke K. Differential expression of three gap junction proteins in developing and mature brain tissues. Proc. Natl. Acad. Sci. U.S.A. 86 (1989) 10148-10152
9. Evans W.H., and Martin P.E. Gap junctions: structure and function (review). Mol. Membr. Biol. 19 (2002) 121-136
10. Fink L., Seeger W., Ermert L., Hanze J., Stahl U., Grimminger F., Kummer W., and Bohle R.M. Real-time quantitative RT-PCR after laser-assisted cell picking. Nat. Med. 4 (1998) 1329-1333
11. Goldberg G.S., Valiunas V., and Brink P.R. Selective permeability of gap junction channels. Biochim. Biophys. Acta 1662 (2004) 96-101
12. +/- mice. Brain Res. 1045 (2005) 107-115
13. Harris A.L. Connexin channel permeability to cytoplasmic molecules. Prog. Biophys. Mol. Biol. 94 (2007) 120-143
14. Hormuzdi S.G., Filippov M.A., Mitropoulou G., Monyer H., and Bruzzone R. Electrical synapses: a dynamic signaling system that shapes the activity of neuronal networks. Biochim. Biophys. Acta 1662 (2004) 113-137
15. Hosny S., and Jennes L. Identification of gap junctional connexin-32 mRNA and protein in gonadotropin-releasing hormone neurons of the female rat. Neuroendocrinology 67 (1998) 101-108
16. Hu L., Olson A.J., Weiner R.I., and Goldsmith P.C. Connexin 26 expression and extensive gap junctional coupling in cultures of GT1-7 cells secreting gonadotropin-releasing hormone. Neuroendocrinology 70 (1999) 221-227
17. Jongsma H.J., and Wilders R. Gap junctions in cardiovascular disease. Circ. Res. 86 (2000) 1193-1197
18. Kanaporis G., Mese G., Valiuniene L., White T.W., Brink P.R., and Valiunas V. Gap junction channels exhibit connexin-specific permeability to cyclic nucleotides. J. Gen. Physiol. 131 (2008) 293-305
19. Kawano H., and Daikoku S. Immunohistochemical demonstration of LHRH neurons and their pathways in the rat hypothalamus. Neuroendocrinology 32 (1981) 179-186
20. Kleber A.G., and Rudy Y. Basic mechanisms of cardiac impulse propagation and associated arrhythmias. Physiol. Rev. 84 (2004) 431-488
21. Krsmanovic L.Z., Stojilkovic S.S., Merelli F., Dufour S.M., Virmani M.A., and Catt K.J. Calcium signaling and episodic secretion of gonadotropin-releasing hormone in hypothalamic neurons. Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 8462-8466
22. Krsmanovic L.Z., Martinez-Fuentes A.J., Arora K.K., Mores N., Navarro C.E., Chen H.C., Stojilkovic S.S., and Catt K.J. Autocrine regulation of gonadotropin-releasing hormone secretion in cultured hypothalamic neurons. Endocrinology 140 (1999) 1423-1431
23. Langlois S., Cowan K.N., Shao Q., Cowan B.J., and Laird D.W. Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes. Mol. Biol. Cell 19 (2008) 912-928
24. Martinez de la Escalera G., Choi A.L., and Weiner R.I. Generation and synchronization of gonadotropin-releasing hormone (GnRH) pulses: intrinsic properties of the GT1-1 GnRH neuronal cell line. Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 1852-1855
25. Martinez de la Escalera G., Choi A.L., and Weiner R.I. Signaling pathways involved in GnRH secretion in GT1 cells. Neuroendocrinology 61 (1995) 310-317
26. Matesic D.F., Germak J.A., Dupont E., and Madhukar B.V. Immortalized hypothalamic luteinizing hormone-releasing hormone neurons express a connexin 26-like protein and display functional gap junction coupling assayed by fluorescence recovery after photobleaching. Neuroendocrinology 58 (1993) 485-492
27. Matsushita T., Oyamada M., Fujimoto K., Yasuda Y., Masuda S., Wada Y., Oka T., and Takamatsu T. Remodeling of cell-cell and cell-extracellular matrix interactions at the border zone of rat myocardial infarcts. Circ. Res. 85 (1999) 1046-1055
28. Mellon P.L., Windle J.J., Goldsmith P.C., Padula C.A., Roberts J.L., and Weiner R.I. Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis. Neuron 5 (1990) 1-10
29. Mellon P.L., Wetsel W.C., Windle J.J., Valenca M.M., Goldsmith P.C., Whyte D.B., Eraly S.A., Negro-Vilar A., and Weiner R.I. Immortalized hypothalamic gonadotropin-releasing hormone neurons. Ciba Found. Symp. 168 (1992) 104-117
30. Nadarajah B., Jones A.M., Evans W.H., and Parnavelas J.G. Differential expression of connexins during neocortical development and neuronal circuit formation. J. Neurosci. 17 (1997) 3096-3111
31. Nagy J.I., Dudek F.E., and Rash J.E. Update on connexins and gap junctions in neurons and glia in the mammalian nervous system. Brain Res. Brain Res. Rev. 47 (2004) 191-215
32. Nakagami T., Tanaka H., Dai P., Lin S.F., Tanabe T., Mani H., Fujiwara K., Matsubara H., and Takamatsu T. Generation of reentrant arrhythmias by dominant-negative inhibition of connexin43 in rat cultured myocyte monolayers. Cardiovasc. Res. 79 (2008) 70-79
33. Niessen H., Harz H., Bedner P., Kramer K., and Willecke K. Selective permeability of different connexin channels to the second messenger inositol 1,4,5-trisphosphate. J. Cell. Sci. 113 Pt 8 (2000) 1365-1372
34. Oyamada M., Oyamada Y., and Takamatsu T. Regulation of connexin expression. Biochim. Biophys. Acta 1719 (2005) 6-23
35. Paruthiyil S., e L.M.M., Conti M., and Weiner R.I. Phosphodiesterase expression targeted to gonadotropin-releasing hormone neurons inhibits luteinizing hormone pulses in transgenic rats. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 17191-17196
36. Pei Z., Oey N.A., Zuidervaart M.M., Jia Z., Li Y., Steinberg S.J., Smith K.D., and Watkins P.A. The acyl-CoA synthetase "bubblegum" (lipidosin): further characterization and role in neuronal fatty acid beta-oxidation. J. Biol. Chem. 278 (2003) 47070-47078
37. Peters N.S., Green C.R., Poole-Wilson P.A., and Severs N.J. Reduced content of connexin43 gap junctions in ventricular myocardium from hypertrophied and ischemic human hearts. Circulation 88 (1993) 864-875
38. Peters A., Heuer H., Schomburg L., De Greef W.J., Visser T.J., and Bauer K. Thyrotropin-releasing hormone gene expression by anterior pituitary cells in long-term cultures is influenced by the culture conditions and cell-to-cell interactions. Endocrinology 138 (1997) 2807-2812
39. Roberts C.B., Best J.A., and Suter K.J. Dendritic processing of excitatory synaptic input in hypothalamic gonadotropin releasing-hormone neurons. Endocrinology 147 (2006) 1545-1555
40. Roberts C.B., Campbell R.E., Herbison A.E., and Suter K.J. Dendritic action potential initiation in hypothalamic gonadotropin-releasing hormone neurons. Endocrinology 149 (2008) 3355-3360
41. Saez J.C., Berthoud V.M., Branes M.C., Martinez A.D., and Beyer E.C. Plasma membrane channels formed by connexins: their regulation and functions. Physiol. Rev. 83 (2003) 1359-1400
42. Sosinsky G.E., Solan J.L., Gaietta G.M., Ngan L., Lee G.J., Mackey M.R., and Lampe P.D. The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodies. Biochem. J. 408 (2007) 375-385
43. Spratt D.I., Carr D.B., Merriam G.R., Scully R.E., Rao P.N., and Crowley Jr. W.F. The spectrum of abnormal patterns of gonadotropin-releasing hormone secretion in men with idiopathic hypogonadotropic hypogonadism: clinical and laboratory correlations. J. Clin. Endocrinol. Metab. 64 (1987) 283-291
44. Terasawa E. Control of luteinizing hormone-releasing hormone pulse generation in nonhuman primates. Cell. Mol. Neurobiol. 15 (1995) 141-164
45. Terasawa E. Cellular mechanism of pulsatile LHRH release. Gen. Comp. Endocrinol. 112 (1998) 283-295
46. Tsukahara S., Maekawa F., Tsukamura H., Hirunagi K., and Maeda K. Morphological characterization of relationship between gap junctions and gonadotropin releasing hormone nerve terminals in the rat median eminence. Neurosci. Lett. 261 (1999) 105-108
47. Usui I., Imamura T., Satoh H., Huang J., Babendure J.L., Hupfeld C.J., and Olefsky J.M. GRK2 is an endogenous protein inhibitor of the insulin signaling pathway for glucose transport stimulation. Embo J. 23 (2004) 2821-2829
48. Uzzaman M., Honjo H., Takagishi Y., Emdad L., Magee A.I., Severs N.J., and Kodama I. Remodeling of gap junctional coupling in hypertrophied right ventricles of rats with monocrotaline-induced pulmonary hypertension. Circ. Res. 86 (2000) 871-878
49. Valiunas V., Polosina Y.Y., Miller H., Potapova I.A., Valiuniene L., Doronin S., Mathias R.T., Robinson R.B., Rosen M.R., Cohen I.S., and Brink P.R. Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions. J. Physiol. 568 (2005) 459-468
50. Vazquez-Martinez R., Shorte S.L., Boockfor F.R., and Frawley L.S. Synchronized exocytotic bursts from gonadotropin-releasing hormone-expressing cells: dual control by intrinsic cellular pulsatility and gap junctional communication. Endocrinology 142 (2001) 2095-2101
51. Weiner R.I., Wetsel W., Goldsmith P., Martinez de la Escalera G., Windle J., Padula C., Choi A., Negro-Vilar A., and Mellon P. Gonadotropin-releasing hormone neuronal cell lines. Front. Neuroendocrinol. 13 (1992) 95-119
52. Yoshida H., Beltran-Parrazal L., Butler P., Conti M., Charles A.C., and Weiner R.I. Lowering cyclic adenosine-3′,5′-monophosphate (cAMP) levels by expression of a cAMP-specific phosphodiesterase decreases intrinsic pulsatile gonadotropin-releasing hormone secretion from GT1 cells. Mol. Endocrinol. 17 (2003) 1982-1990
53. Zemljic-Harpf A.E., Miller J.C., Henderson S.A., Wright A.T., Manso A.M., Elsherif L., Dalton N.D., Thor A.K., Perkins G.A., McCulloch A.D., and Ross R.S. Cardiac-myocyte-specific excision of the vinculin gene disrupts cellular junctions, causing sudden death or dilated cardiomyopathy. Mol. Cell. Biol. 27 (2007) 7522-7537