Effective elimination of nucleic acids from bacterial protein samples for optimized blue native polyacrylamide gel electrophoresis

Electrophoresis

Volumn 30, Issue 14, 2009, Pages 2454-2459

  Liang, Jingdan ^a   Niu, Qiule ^a   Xu, Xinping ^b   Luo, Yuanming ^b   Zhou, Xiufen ^a   Deng, Zixin ^a   Wang, Zhijun ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

Author keywords

Blue native polyacrylamide gel electrophoresis; Nucleic acid binding protein; Polyethyleneimine precipitation; Sample preparation; Streptomycin sulfate

Indexed keywords

ANTIBIOTICS; BIOLOGICAL MEMBRANES; BIOMOLECULES; ELECTROPHORESIS; ESCHERICHIA COLI; NUCLEIC ACIDS; PROTEINS;

ACID-BINDING PROTEINS; BACTERIAL PROTEIN; BLUE NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NUCLEIC ACID-BINDING PROTEIN; POLY(ETHYLENEIMINE); POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYETHYLENEIMINE PRECIPITATION; PROTEIN SAMPLES; SAMPLE PREPARATION; STREPTOMYCIN SULPHATE;

SULFUR COMPOUNDS;

BACTERIAL PROTEIN; MEMBRANE PROTEIN; NUCLEIC ACID BINDING PROTEIN; POLYACRYLAMIDE; POLYETHYLENEIMINE; STREPTOMYCIN SULFATE; BACTERIAL DNA; BACTERIAL RNA; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; NUCLEIC ACID; STREPTOMYCIN;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; ESCHERICHIA COLI; GEL ELECTROPHORESIS; NONHUMAN; PRECIPITATION; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEOMICS; STREPTOMYCES LIVIDANS; ULTRACENTRIFUGATION; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIAL PROTEINS; DNA, BACTERIAL; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; NUCLEIC ACIDS; PRECIPITATION; RNA, BACTERIAL; STREPTOMYCES LIVIDANS; STREPTOMYCIN; ULTRACENTRIFUGATION;

EID: 70350435615     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900026     Document Type: Article

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