The effect of Cucumber mosaic virus 2b protein to transient expression and transgene silencing mediated by agro-infiltration

Plant Pathology Journal

Volumn 24, Issue 3, 2008, Pages 296-304

  Choi, Min Sue ^a   Yoon, In Sun ^a   Rhee, Yong ^b   Choi, Seung Kook ^a   Lim, Sun Hyung ^a   Won, So Youn ^a   Lee, Yeon Hee ^a   Choi, Hong Soo ^a   Lee, Suk Chan ^b   Kim, Kook Hyung ^c   Lomonossoff, George ^d   Sohn, Seong Han ^a  

^a National Institute of Animal Science   (South Korea)

^b Sungkyunkwan University   (South Korea)

^c Seoul National University   (South Korea)

^d JOHN INNES CENTRE   (United Kingdom)

Author keywords

Agro infiltration; Cucumber mosaic virus 2b; GFP; Post transcriptional gene silencing

Indexed keywords

EID: 70350248333     PISSN: 15982254     EISSN: 20939280     Source Type: Journal    
DOI: 10.5423/PPJ.2008.24.3.296     Document Type: Article

References (25)

1. Abdal-Aziz, S. A., Pliego-Alfaro, F., Quesada, M. A. and Mercado, J. A. 2006. Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant. J. Biosci. Bioeng. 6:508-510.
2. Baumberger, N. and Baulcombe, D. C. 2005. Arabidopsis ARGONAUTE1 is an RNA silencer that selectively recruits miRNAs and short interfering RNAs. Proc. Natl. Acad. Sci. USA 102:11928-11933.
3. Bernstein, E., Caudy, A. A., Hammond, S. M. and Hannon, G J. 2001. Role of a bidentate ribonuclease in the initiation step of RNA interference. Nature 409:363-366.
4. De Buck, S., De Wilde, C., Van Montagu, M. and Depicker A. 2000. T-DNA vector backbone sequences are frequently integrated into genome of transgenic plants obtained by Agrobacterium-mediated transformation. Mol. Breeding 6:459-468.
5. Elbashir, S. M., Lendeckel, W. and Tuschl, T. 2001. RNA interference is mediated by 21-and 22-nucleotide RNAs. Genes Dev. 15:188-200.
6. Fisher, R., Vaquero-Martin, C., Sack, M., Drossard, J., Emans, N. and Crmmandeur, U. 1999. Towards molecular farming in the future: transient protein expression in plants. Biotechnol. Appl. Biochem. 30:113-116.
7. Goto, K., Kobori, T., Kosaka, Y., Natsuaki, T. and Masuta, C. 2007. Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Plant Cell Physiol. 48:1050-1060.
8. Hamilton, A. J. and Baulcombe, D. C. 1999. A species of small antisence RNA in post-transcriptional gene silencing in plants. Science 286:950-952.
9. Hellens, R. P., Edwards, E. A., Leyland, N. R., Bean, S. and Mullineaux, P. M. 2000. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol. 42:819-832.
10. Johansen, L. K. and Carrington, J. C. 2001. Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol. 126:930-938.
11. Kapila, J., de Rycke, R., van Montagu, M. and Angenon, G 1997. An Agrobacterium-meáialed transient gene expression system for intact leaves. Plant Sci. 122:101-108.
12. Kim, S.-R., Lee, J., Jun, S.-H., Park, S., Kang, H.-G, Kwon, S. and An, G 2003. Transgenic structures in T-DNA-inserted rice plants. Plant Mol. Biol 52:761-773.
13. Kuraya, Y., Ohta, S., Fukuda, M., Hiei, Y., Murai, N., Hamada, K., Ueki, J., Imaseki, H. and Komai, T. 2004. Suppression of transfer of non-T-DNA 'vector backbone' sequences by multiple left border repeats in vectors for transformation of higher plants mediated by Agrobacterium tumefaciens. Mol. Breeding 14:309-320.
14. Lindbo, J. A., Silva-Rosales, L., Proebsting, W. M. and Dougherty, W. G 1993. Induction of highly specific antiviral state in transgenic plants: Implications for regulation of gene expression and virus resistance. Plant Cell 5:1749-1759.
15. Mayers, C. N., Palukaitis, P. and Carr, J. P. 2000. Subcelluar distribution analysis of the cucumber mosaic virus 2b protein. J. Gen. Viol. 81:9-226.
16. McCormac, A. C., Elliott, M. C. and Chen, D. F. 1998. A simple method for the production of highly competent cells of Agrobacterium for transformation via electroporation. Mol. Biotech. 9:155-159.
17. Meins, F. 2000. RNA degradation and models for post-transcriptional gene silencing. Plant Mol. Biol. 43:261-273.
18. Ratcliff, F. G, MacFarlane, S. A. and Baulcombe, D. C. 1999. Gene silencing without DNA. RNA-meditated cross-protection between viruses. Plant Cell 11:794-798.
19. Schubert, D., Lechtenberg, B., Forsbach, A., Gils, M., Bahadur, S. and Schmidt, R. 2004. Silencing in Arabidopsis T-DNA Transformants: The Predominant Role of a Gene-Specific RNA Sensing Mechanism versus Position Effects. Plant Cell 16:2561-2572.
20. Tzfira, T., Frankman, L. R., Vaidya, M. and Citovsky, V. 2003. Site-specific integration if Agrobacterium tumefaciens T-DNA via double-stranded intermediates. Plant Physiol. 133:1011-1023.
21. Vaucheret, H., Béclin, C. and Fagard, M. 2001. Post-transcriptional gene silencing in plants. J. Cell Sci. 114:3083-3091.
22. Vionnet, O., Pinto, Y. M. and Baulcombe, D. C. 1999. Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc. Natl. Acad. Sci. USA 96:14147-14152.
23. Voinnet, O. 2001. RNA silencing as a plant immune system against viruses. Trends Genet. 17:449-459.
24. Voinnet, O., Rivas, S., Mestre, P. and Baulcombe, D. C. 2003. An enhanced transient expression system in plants based on suppression of gene silencing by the p 19 protein of tomato bushy stunt virus. Plant J. 33:949-956.
25. Zhang, X., Yuan, Y.-R., Pei, Y., Lin, S.-S., Tuschl, T., Patel, D. J. and Chua, N.-H. 2008. Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonautel cleavage activity to counter plant defence. Genes Develop. 20:3255-3268.