A simple method for the production of highly competent cells of Agrobacterium for transformation via electroporation

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 9, Issue 2, 1998, Pages 155-159

  McCormac, A C ^a   Elliott, M C ^a   Chen, D F ^a  

^a DE MONTFORT UNIVERSITY   (United Kingdom)

Author keywords

Agrobacterium; Binary plasmids; Competence; Electroporation; Selection; Transformation

Indexed keywords

ANTIBIOTIC AGENT; PLASMID DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; CELL TRANSFORMATION; ELECTROPORATION; NONHUMAN; RHIZOBIUM;

AGROBACTERIUM; BACTERIA (MICROORGANISMS);

EID: 0031716027     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02760816     Document Type: Article

References (6)

1. Nagel, R., Elliott, A., Masel, A., Birch, R. G., and Manners, J. M. (1990) Electroporation of binary Ti plasmid vector into Agrobacterium tumefaciens and Agrobacterium rhizogenes. FEMS Microbiol. Lett. 67, 325.
2. McCormac, A. C., Elliott, M. C., and Chen D. F. (1997) pBECKS: A flexible series of binary vectors for Agrobacterium-mediated plant transformation. Mol. Biotechnol. (in press).
3. Hood, E. E., Helmer, G. L., Fraley, R. T., and Chilton, M.-D. (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J. Bacteriology 168, 1291-1301.
4. Hoekema, A., Hirsch, P. R., Hooykaas, P. J. J., and Schilperoort, R. A. (1983) A binary vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303, 179,180.
5. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory press. Cold Spring Harbor, New York.
6. Chilton, M.-D., Currier, T. C., Farrand, S. K., Bendich, A. J., Gordon, M. P., and Nester, E. W. (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumours. Proc. Natl. Acad. Sci. USA 71, 3672-3676.