Mechanism of pH-sensitive polymer-assisted protein refolding and its application in TGF-β1 and KGF-2

Biotechnology Progress

Volumn 25, Issue 5, 2009, Pages 1387-1395

  Huang, Zhifeng ^a,b,c   Ni, Chunyan ^a   Zhou, Xiangtian ^a   Liu, Yanlong ^a   Tan, Yi ^a   Xiao, Jian ^a   Feng, Wenke ^a   Li, Xiaokun ^a,c   Yang, Shulin ^a  

^a NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b WENZHOU MEDICAL UNIVERSITY   (China)

^c Nanyang Technological University   (Singapore)

Author keywords

Eudragit; KGF 2; Lysozyme; Protein refolding; TGF 1

Indexed keywords

BIO-CONJUGATION; BIO-MOLECULAR; ELECTROSTATIC INTERACTIONS; EUDRAGIT; EUDRAGIT S; HIGH CONCENTRATION; HYDROPHOBIC RESIDUES; ION EXCHANGE CHROMATOGRAPHY; KGF-2; LYSOZYME; PH SENSITIVE; PH-RESPONSIVE POLYMER; PROTEIN REFOLDING; PROTEIN STRUCTURES; REFOLDING;

CHROMATOGRAPHIC ANALYSIS; HYDROPHOBICITY; ION CHROMATOGRAPHY; ION EXCHANGE; POLYMERS;

ENZYMES;

FIBROBLAST GROWTH FACTOR 10; HEN EGG LYSOZYME; LYSOZYME; METHYLMETHACRYLATE METHACRYLIC ACID COPOLYMER; METHYLMETHACRYLATE-METHACRYLIC ACID COPOLYMER; POLYMETHACRYLIC ACID DERIVATIVE; TRANSFORMING GROWTH FACTOR BETA1;

ANIMAL; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ISOELECTRIC POINT; METABOLISM; PH; PROTEIN FOLDING; SPECTROFLUOROMETRY;

ANIMALS; CIRCULAR DICHROISM; FIBROBLAST GROWTH FACTOR 10; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; MURAMIDASE; POLYMETHACRYLIC ACIDS; PROTEIN FOLDING; SPECTROMETRY, FLUORESCENCE; TRANSFORMING GROWTH FACTOR BETA1;

EID: 70350168424     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.218     Document Type: Article

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