Assessing the limitations to terpenoid indole alkaloid biosynthesis in catharanthus roseus hairy root cultures through gene expression profiling and precursor feeding

Biotechnology Progress

Volumn 25, Issue 5, 2009, Pages 1289-1296

  Goklany, Sheba ^a   Loring, Ralph H ^b   Glick, James ^b   Lee Parsons, Carolyn W T ^a  

^a Northeastern University   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

Alkaloid; Catharanthus roseus; Gene expression; Hairy root cultures; Methyl jasmonate; Precursor feeding; Quantitative RT PCR

Indexed keywords

ALKALOID; CATHARANTHUS ROSEUS; HAIRY ROOT CULTURES; METHYL JASMONATE; PRECURSOR FEEDING; QUANTITATIVE RT-PCR;

BIOACTIVITY; BIOCHEMISTRY; BIOSYNTHESIS; DIFFERENTIAL AMPLIFIERS; DRUG PRODUCTS; FEEDING; GENES; SERPENTINE; SILICATE MINERALS; TIMING CIRCUITS; TRANSCRIPTION;

CELL CULTURE;

IRIDOID; LOGANIN; MESSENGER RNA; PLANT RNA; RAUWOLFIA ALKALOID; TRYPTAMINE; TRYPTAMINE DERIVATIVE;

ARTICLE; CATHARANTHUS; GENE EXPRESSION PROFILING; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; PLANT ROOT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE CULTURE TECHNIQUE;

CATHARANTHUS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENE EXPRESSION PROFILING; IRIDOIDS; METABOLIC NETWORKS AND PATHWAYS; PLANT ROOTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, PLANT; SECOLOGANIN TRYPTAMINE ALKALOIDS; TISSUE CULTURE TECHNIQUES; TRYPTAMINES;

CATHARANTHUS ROSEUS;

EID: 70350139483     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.204     Document Type: Article

References (28)

1. van der Heijden R, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R. The Catharanthus alkaloids: pharmacognosy and biotechnology. Curr Med Chem. 2004;11:607-628.
2. van der Fits L, Memelink J. ORCA3, a jasmonate-responsive transcriptional regulator of plant primary and secondary metabolism. Science. 2000;289:295-297.
3. Hong SB, Hughes EH, Shanks JV, San KY, Gibson SI. Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots. Biotechnol Prog. 2003;19: 1105-1108.
4. El-Sayed M, Verpoorte R. Catharanthus terpenoid indole alkaloids: biosynthesis and regulation. Phytochem Rev. 2007;6:277-305.
5. Loyola-Vargas VM, Galaz-Avalos RM, Ku-Cauich R. Catharanthus biosynthetic enzymes: the road ahead. Phytochem Rev. 2007;6:307-339.
6. Baíza A, Quiroz-Moreno A, Ruíz J, Loyola-Vargas V. Genetic stability of hairy root cultures of Datura stramonium. Plant Cell Tissue Organ Cult. 1999;59:9-17.
7. Shanks JV, Morgan J. Plant 'hairy root' culture. Curr Opin Biotechnol. 1999;10:151-155.
8. Radman R, Saez T, Bucke C, Keshavarz T. Elicitation of plants and microbial cell systems. Biotechnol Appl Biochem. 2003;37: 91-102.
9. Memelink J, Verpoorte R, Kijne JW. ORCAnization of jasmonate-responsive gene expression in alkaloid metabolism. Trends Plant Sci. 2001;6:212-219.
10. Vazquez-Flota F, De Luca V, Carrillo-Pech M, Canto-Flick A, de Lourdes Miranda-Ham M. Vindoline biosynthesis is transcriptionally blocked in Catharanthus roseus cell suspension cultures. Mol Biotechnol. 2002;22:1-8.
11. Menke FL, Parchmann S, Mueller MJ, Kijne JW, Memelink J. Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthetic genes in Catharanthus roseus. Plant Physiol. 1999;119:1289-1296.
12. Gundlach H, Muller MJ, Kutchan TM, Zenk MH. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA. 1992;89:2389-2393.
13. Rischer H, Oresic M, Seppanen-Laakso T, Katajamaa M, Lammertyn F, Ardiles-Diaz W, Van Montagu MC, Inze D, Oksman-Caldentey KM, Goossens A. Gene-to-metabolite networks for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells. Proc Natl Acad Sci USA. 2006;103:5614-5619.
14. Collu G, Unver N, Peltenburg-Looman AM, van der Heijden R, Verpoorte R, Memelink J. Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis. FEBS Lett. 2001;508:215-220.
15. Lee-Parsons CWT, Royce AJ. Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures. Plant Cell Rep. 2006;25:607-612.
16. Morgan JA, Shanks JV. Determination of metabolic rate-limitations by precursor feeding in Catharanthus roseus hairy root cultures. J Biotechnol. 2000;79:137-145.
17. Whitmer S, Canel C, Hallard D, Goncalves C, Verpoorte R. Influence of precursor availability on alkaloid accumulation by transgenic cell line of Catharanthus roseus. Plant Physiol. 1998;116:853-857. (Pubitemid 128482314)
18. Whitmer S, van der Heijden R, Verpoorte R. Effect of precursor feeding on alkaloid accumulation by a tryptophan decarboxylase over-expressing transgenic cell line T22 of Catharanthus roseus. J Biotechnol. 2002;96:193-203. (Pubitemid 34556961)
19. Whitmer S, van der Heijden R, Verpoorte R. Effect of precursor feeding on alkaloid accumulation by a strictosidine synthase over-expressing transgenic cell line S1 of Catharanthus roseus. Plant Cell Tissue Organ Cult. 2002;69:85-93. (Pubitemid 34432022)
20. Goklany S. Monitoring the expression of TIA pathway genes in Catharanthus roseus hairy root cultures under optimum elicitation conditions. Master's thesis, Northeastern University, Boston, 2007.
21. Chang S, Puryear J, Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep. 1993; 11:113-116.
22. Dutta A, Batra J, Pandey-Rai S, Singh D, Kumar S, Sen J. Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don. Planta. 2005;220:376-383.
23. Dagnino D, Schripsema J, Verpoorte R. Analysis of several iridoid and indole precursors of terpenoid indole alkaloids with a single HPLC run. Planta Med. 1996;62:278-280. (Pubitemid 126425824)
24. 2+ level. Plant Cell Rep. 2005;24:677-682.
25. Gangl ET, Annan M, Spooner N, Vouros P. Reduction of signal suppression effects in ESI-MS using a nanosplitting device. Anal Chem. 2001;73:5635-5644.
26. Rijhwani SK, Shanks JV. Effect of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol Prog. 1998;14:442-449.
27. Zhao J, Zhu W-H, Hu Q. Effects of light and plant growth regulators on the biosynthesis of vindoline and other indole alkaloids in Catharanthus roseus callus cultures. Plant Growth Regul. 2001;33:43-49.
28. Loyola-Vargas VM, Mendez-Zeel M, Monforte-Gonzalez M, Miranda-Ham MDL. Serpentine accumulation during greening in normal and tumor tissues of Catharanthus roseus. J Plant Physiol. 1992;140:213-217.