ORCA3, a jasmonate-responsive transcriptional regulator of plant primary and secondary metabolism

Science

Volumn 289, Issue 5477, 2000, Pages 295-297

  Van Der Fits, Leslie ^a   Memelink, Johan ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALOID DERIVATIVE; INDOLE DERIVATIVE; JASMONIC ACID; TRANSCRIPTION FACTOR; VINBLASTINE;

ARTICLE; BIOSYNTHESIS; ELECTROPHORETIC MOBILITY; GENE CONTROL; PHYTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ISOLATION; STRESS;

ACETIC ACIDS; ANGIOSPERMS; CELL LINE; CYCLOPENTANES; DNA, BACTERIAL; GENE EXPRESSION REGULATION, PLANT; HOMEODOMAIN PROTEINS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PLANT GROWTH REGULATORS; PLANT PROTEINS; TRANSCRIPTION FACTORS; VINCA ALKALOIDS;

CATHARANTHUS ROSEUS; EMBRYOPHYTA;

EID: 0034647914     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5477.295     Document Type: Article

References (22)

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8. Cell cultures of C. roseus grown as described in (18) were transformed by particle bombardment (C. roseus cell line MP183L) (18) or with an improved Agrobacterium tumefaciens strain (C. roseus cell line BIX) (9).
9. L. van der Fits and J. Memelink, data not shown.
10. Ten micrograms of genomic DNA from line 46 were digested with Xba I, self-ligated, and electroporated into Escherichia coli strain NM554. Cells were selected on carbenicillin.
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13. Polymerase chain reactions (PCR) were performed on a C. roseus root cDNA library in lambda ZAPII (Stratagene) with primers based on Orca3 genomic sequences (Fig. 2B). Primer combinations OR1 with M13-40 and OR4 with T3 primer were used to isolate 3′ and 5′ cDNA parts, respectively. Primers OR5 and OR6, based on cDNA sequences, were used to generate a complete Orca3 ORF.
14. One microgram of genomic C. roseus DNA was digested with Eco RI and self-ligated and inverse-PCR (I-PCR) was performed with primers OR5 and OR8 (Fig. 2B).
15. Northern blots were hybridized with C. roseus cDNAs as follows (see Fig. 1 legend for abbreviations; GenBank accession numbers are in parentheses): Tdc (Acc. No. X67662), Str (Acc. No. X61932), Sgd (Acc. No. AF112888), G10h (Acc. No. AJ251269),
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22. The authors thank S. Whitmer and A. Hermans for assistance with high-performance liquid chromatography analysis; P. Hock for preparing the figures; W. de Winter for assistance with tissue culturing; D. Vom Endt for I-PCR; and J. Kijne, R. Verpoorte, and F. Menke for valuable discussions. Supported by the Ministry of Economic Affairs; the Ministry of Education, Culture, and Science; and the Ministry of Agriculture, Nature Management, and Fishery in the framework of an industrial relevant research program of the Netherlands Association of Biotechnology Centres in the Netherlands (ABON).