High quality protein microarray using in situ protein purification

BMC Biotechnology

Volumn 9, Issue , 2009, Pages 72-

  Kwon, Keehwan ^a   Grose, Carissa ^a   Pieper, Rembert ^a   Pandya, Gagan A ^a   Fleischmann, Robert D ^a   Peterson, Scott N ^a  

^a J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEMONSTRATION PROJECT; ESCHERICHIA COLI CELLS; FREEZE-THAW CYCLES; FUSION TAG; GOLD STANDARDS; HIGH QUALITY; HIGH THROUGHPUT; HIGH-DENSITY; HIS-TAGGED PROTEINS; HUMAN PATIENTS; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHIES; IMMUNOPROTEOMICS; IN-SITU; IN-SITU PURIFICATION; IN-VIVO; METAL CHELATE; NOVEL PROTEINS; PHOSPHATE BUFFERS; PROTEIN EXPRESSIONS; PROTEIN MICROARRAY; PROTEIN PURIFICATION; PROTEIN PURITY; PROTEIN SOLUBILITY; PROTEIN-PROTEIN INTERACTIONS; PURIFICATION PROCESS; PURIFIED PROTEIN; RECOMBINANT PROTEIN; SLIDE SURFACE; SMALL SIZE; STREPTOCOCCUS PNEUMONIAE; TWEEN-20;

AFFINITY CHROMATOGRAPHY; ANTIBODIES; ANTIGENS; BIOASSAY; BIOCHIPS; BUFFER STORAGE; CELL MEMBRANES; CHELATION; CHROMATOGRAPHIC ANALYSIS; COATINGS; ESCHERICHIA COLI; GOLD DEPOSITS; ION CHROMATOGRAPHY; METAL IONS; OPTIMIZATION; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PURIFICATION; RESINS; SODIUM CHLORIDE;

METAL REFINING;

ANTISERUM; BACTERIAL ANTIGEN; BACTERIAL PROTEIN; BUFFER; COPPER; HEXAHISTIDINE; MACROGOL; METAL CHELATE; NITROGEN; POLYSORBATE 20; POTASSIUM CHLORIDE; RECOMBINANT PROTEIN; RESIN; SODIUM CHLORIDE;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ESCHERICHIA COLI; FREEZE THAWING; GOLD STANDARD; HIGH THROUGHPUT SCREENING; HUMAN; IN VIVO STUDY; MATERIAL COATING; NONHUMAN; PH; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN IMMOBILIZATION; PROTEIN MICROARRAY; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; SOLUBILITY; STREPTOCOCCUS PNEUMONIA;

BACTERIAL PROTEINS; CHELATING AGENTS; COPPER; ESCHERICHIA COLI; NICKEL; PROTEIN ARRAY ANALYSIS; PROTEOMICS; RECOMBINANT PROTEINS; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; STREPTOCOCCUS PNEUMONIAE;

EID: 70350108624     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-72     Document Type: Article

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