Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad- 14C,4-2H] NADPH and (4R)-[Ad-3H,4- 2H] NADPH

Journal of Labelled Compounds and Radiopharmaceuticals

Volumn 52, Issue 11, 2009, Pages 463-466

  Yahashiri, Atsushi ^a   Sen, Arundhuti ^a   Kohen, Amnon ^a  

^a E11 SSH   (United States)

Author keywords

Dihydrofolate reductase; Isotopic labeling; Kinetic isotope effect; Labeled NADPH; Nicotinamide adenine dinucleotide 2 phosphate; Thermoanaerobium brockii alcohol dehydrogenase

Indexed keywords

AMIDES; ENZYMES; KINETICS;

ALCOHOL DEHYDROGENASE; DIHYDROFOLATE REDUCTASE; ISOTOPIC LABELING; KINETIC ISOTOPES EFFECTS; LABELED NADPH; MICROSCALE SYNTHESIS; NAD +; NICOTINAMIDE ADENINE DINUCLEOTIDE 2′ PHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDES; THERMOANAEROBIUM BROCKII ALCOHOL DEHYDROGENASE;

DEUTERIUM;

4 (ADENOSINE CARBON 14,4 DEUTERIUM) NADPH; 4 (ADENOSINE TRITIUM,4 DEUTERIUM) NADPH; ALCOHOL DEHYDROGENASE; CARBON 14; DEUTERIUM; NICOTINAMIDE ADENINE DINUCLEOTIDE KINASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; DRUG SYNTHESIS; ISOTOPE LABELING; PHOSPHORYLATION; THERMOANAEROBACTERIACEAE; THERMOANAEROBIUM BROCKII;

THERMOANAEROBACTER BROCKII;

EID: 70350077264     PISSN: 03624803     EISSN: 10991344     Source Type: Journal    
DOI: 10.1002/jlcr.1660     Document Type: Article

References (15)

1. L. Wang, S. Tharp, T. Selzer, S. J. Benkovic, A. Kohen, Biochemistry 2006, 45, 1383-1392.
2. L. Wang, N. M. Goodey, S. J. Benkovic, A. Kohen, Proc. Nat. Acad. Sci. USA, 2006, 103, 15753-15758.
3. L. Wang, N. M. Goodey, S. J. Benkovic, A. Kohen, Philos. Trans. R. Soc. London, Ser. B 2006, 361, 1307-1315.
4. R. S. Sikorski, L. Wang, K. A. Markham, P. T. R. Rajagopalan, S. J. Benkovic, A. Kohen, J. Am. Chem. Soc. 2004, 126, 4778-4779.
5. A. Sen, A. Kohen, In Quantum Tunneling in Enzyme Catalyzed Reactions (Eds.: R. Allemann, N. Scrutton), Royal Society of Chemistry, London, UK, 2008, pp. 161-178.
6. D. W. Parkin, In Enzyme Mechanism from Isotope Effects (Ed.: P. F. Cook), CRC Press, Boca Raton, FL, 1991, pp. 269-290.
7. D. B. Northrop, In Enzyme Mechanism from Isotope Effects (Ed.: P. F. Cook), CRC Press, Boca Raton, FL, 1991, pp. 181-202.
8. A. Kohen, In Isotope Effects in Chemistry and Biology (Eds.: A. Kohen, H. H. Limbach), Taylor and Francis, CRC Press, Boca Raton, FL, 2006, pp. 743-764.
9. J. A. McCracken, L. Wang, A. Kohen, Anal. Biochem. 2004, 324, 131-136.
10. J. A. McCracken, L. Wang, A. Kohen, Anal. Biochem. 2003, 324, 131-136.
11. N. Agrawal, A. Kohen, Anal. Biochem. 2003, 322, 179-184.
12. K. A. Markham, R. S. Sikorski, A. Kohen, Anal. Biochem. 2003, 322, 26-32.
13. K. A. Markham, A. Kohen, Curr. Anal. Chem. 2006, 2, 379-388.
14. A. Yahashiri, E. E. Howell, A. Kohen, Chem. Phys. Chem. 2008, 9, 980-982.
15. L. Melander, H. W. Saunders, Reaction Rates of Isotopic Molecules, Krieger Pub. Co., Malabar, FL, 1987.