MicroRNA isolation and stability in stored RNA samples

Biochemical and Biophysical Research Communications

Volumn 390, Issue 1, 2009, Pages 1-4

  Mraz, M ^a   Malinova, K ^a   Mayer, J ^a   Pospisilova, S ^a  

^a UNIVERSITY HOSPITAL BRNO   (Czech Republic)

Author keywords

miRNA cDNA stability; miRNA isolation; miRNA stability

Indexed keywords

MICRORNA;

B LYMPHOCYTE; CHRONIC LYMPHATIC LEUKEMIA; GENE EXPRESSION; HUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; RNA STABILITY; SHORT SURVEY; STORAGE; TEMPERATURE;

B-LYMPHOCYTES; GUANIDINES; HUMANS; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; MICRORNAS; PHENOLS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA STABILITY; SOLUTIONS; SPECIMEN HANDLING;

EID: 70349952140     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.09.061     Document Type: Short Survey

References (25)

1. Lagos-Quintana M., Rauhut R., Lendeckel W., and Tuschl T. Identification of novel genes coding for small expressed RNAs. Science 294 (2001) 853-858
2. Lee Y., Jeon K., Lee J.T., Kim S., and Kim V.N. MicroRNA maturation: stepwise processing and subcellular localization. EMBO J. 21 (2002) 4663-4670
3. Yi R., Qin Y., Macara I.G., and Cullen B.R. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev. 17 (2003) 3011-3016
4. Lund E., Guttinger S., Calado A., Dahlberg J.E., and Kutay U. Nuclear export of microRNA precursors. Science 303 (2004) 95-98
5. Filipowicz W., Bhattacharyya S.N., and Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?. Nat. Rev. Genet. 9 (2008) 102-114
6. Esquela-Kerscher, F.J. Slack, Oncomirs - microRNAs with a role in cancer, Nat. Rev. Cancer 6 (2006) 259-269.
7. Visone R., and Croce C.M. MiRNAs and cancer. Am. J. Pathol. 174 (2009) 1131-1138
8. Mraz M., Pospisilova S., Malinova K., Slapak I., and Mayer J. MicroRNAs in chronic lymphocytic leukemia pathogenesis and disease subtypes. Leuk. Lymphoma 50 (2009) 506-509
9. Lu J., Getz G., Miska E.A., Alvarez-Saavedra E., Lamb J., Peck D., Sweet-Cordero A., Ebert B.L., Mak R.H., Ferrando A.A., Downing J.R., Jacks T., Horvitz H.R., and Golub T.R. MicroRNA expression profiles classify human cancers. Nature 435 (2005) 834-838
10. Bravo V., Rosero S., Ricordi C., and Pastori R.L. Instability of miRNA and cDNAs derivatives in RNA preparations. Biochem. Biophys. Res. Commun. 353 (2007) 1052-1055
11. Yin J.Q., and Zhao R.C. Identifying expression of new small RNAs by microarrays. Methods 43 (2007) 123-130
12. Yin J.Q., Zhao R.C., and Morris K.V. Profiling microRNA expression with microarrays. Trends Biotechnol. 26 (2008) 70-76
13. Shingara J., Keiger K., Shelton J., Laosinchai-Wolf W., Powers P., Conrad R., Brown D., and Labourier E. An optimized isolation and labeling platform for accurate microRNA expression profiling. RNA 11 (2005) 1461-1470
14. Castoldi M., Benes V., Hentze M.W., and Muckenthaler M.U. MiChip: a microarray platform for expression profiling of microRNAs based on locked nucleic acid (LNA) oligonucleotide capture probes. Methods 43 (2007) 146-152
15. Castoldi M., Schmidt S., Benes V., Hentze M.W., and Muckenthaler M.U. MiChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes. Nat. Protoc. 3 (2008) 321-329
16. Liu C.G., Spizzo R., Calin G.A., and Croce C.M. Expression profiling of microRNA using oligo DNA arrays. Methods 44 (2008) 22-30
17. Schmittgen T.D., Lee E.J., Jiang J., Sarkar A., Yang L., Elton T.S., and Chen C. Real-time PCR quantification of precursor and mature microRNA. Methods 44 (2008) 31-38
18. Wang W.X., Wilfred B.R., Baldwin D.A., Isett R.B., Ren N., Stromberg A., and Nelson P.T. Focus on RNA isolation: obtaining RNA for microRNA (miRNA) expression profiling analyses of neural tissue. Biochim. Biophys. Acta 1779 (2008) 749-757
19. Doleshal M., Magotra A.A., Choudhury B., Cannon B.D., Labourier E., and Szafranska A.E. Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues. J. Mol. Diagn. 10 (2008) 203-211
20. Botling J., Edlund K., Segersten U., Tahmasebpoor S., Engström M., Sundström M., Malmström P.U., and Micke P. Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue. Diagn. Mol. Pathol. 18 (2009) 44-52
21. Staal F.J., Cario G., Cazzaniga G., Haferlach T., Heuser M., Hofmann W.K., Mills K., Schrappe M., Stanulla M., Wingen L.U., van Dongen J.J., and Schlegelberger B. Consensus guidelines for microarray gene expression analyses in leukemia from three European leukemia networks. Leukemia 20 (2006) 1385-1392
22. Leonard S., Logel J., Luthman D., Casanova M., Kirch D., and Freedman R. Biological stability of mRNA isolated from human postmortem brain collections. Biol. Psychiatry 33 (1993) 456-466
23. Van Deerlin V.M., Gill L.H., and Nelson P.T. Optimizing gene expression analysis in archival brain tissue. Neurochem. Res. 27 (2002) 993-1003
24. Nelson P.T., Wang W.X., Wilfred B.R., and Tang G. Technical variables in high-throughput miRNA expression profiling: much work remains to be done. Biochim. Biophys. Acta 1779 (2008) 758-765
25. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159