Characterization of a novel α4/4-conotoxin, Qc1.2, from vermivorous Conus quercinus

Acta Biochimica et Biophysica Sinica

Volumn 41, Issue 10, 2009, Pages 858-864

  Peng, Can ^a   Chen, Weihua ^a   Han, Yuhong ^b   Sanders, Tanya ^c   Chew, Geoffrey ^c   Liu, Jing ^c   Hawrot, Edward ^c   Chi, Chengwu ^a,b   Wang, Chunguang ^a  

^a TONGJI UNIVERSITY   (China)

^b INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^c BROWN UNIVERSITY   (United States)

Author keywords

4 4 conotoxin; Nicotinic acetylcholine receptor subtype; Post translational modification; Xenopus oocyte

Indexed keywords

CONUS QUERCINUS; RATTUS;

CALCIUM CHANNEL BLOCKING AGENT; CONOTOXIN; CONOTOXIN QC1.2, CONUS QUERCINUS; NICOTINIC RECEPTOR; PEPTIDE; POTASSIUM CHANNEL KV1.6;

ANIMAL; ARTICLE; CHEMISTRY; CHINA; CONUS SNAIL; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN ENGINEERING; PROTEIN PROCESSING; SYNTHESIS;

ANIMALS; CALCIUM CHANNEL BLOCKERS; CHINA; CONOTOXINS; CONUS SNAIL; KV1.6 POTASSIUM CHANNEL; PEPTIDES; PROTEIN ENGINEERING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTORS, NICOTINIC;

EID: 70349604421     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gmp077     Document Type: Article

References (25)

1. Nicke A, Wonnacott S and Lewis RJ. a-conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes. Eur J Biochem 2004, 271: 2305-2319.
2. Jones S, Sudweeks S and Yakel JL. Nicotinic receptors in the brain: correlating physiology with function. Trends Neurosci 1999, 22: 555-561.
3. Lindstrom J. Nicotinic acetylcholine receptors in health and disease. Mol Neurobiol 1997, 15: 193-222.
4. Lloyd GK and Williams M. Neuronal nicotinic acetylcholine receptors as novel drug targets. J Pharmacol Exp Ther 2000, 292: 461-467.
5. Azam L, Dowell C, Watkins M, Stitzel JA, Olivera BM and McIntosh JM. a-conotoxin BuIA, a novel peptide from Conus bullatus, distinguishes among neuronal nicotinic acetylcholine receptors. J Biol Chem 2005, 280: 80-87.
6. Craik DJ and Adams DJ. Chemical modification of conotoxins to improve stability and activity. ACS Chem Biol 2007, 2: 457-468.
7. Martinez JS, Olivera BM, Gray WR, Craig AG, Groebe DR, Abramson SN and McIntosh JM. a-Conotoxin EI, a new nicotinic acetylcholine receptor antagonist with novel selectivity. Biochemistry 1995, 34: 14519-14526.
8. López-Vera E, Aguilar MB, Schiavon E, Marinzi C, Ortiz E, Restano Cassulini R and Batista CV, et al. Novel a-conotoxins from Conus spurius and the a-conotoxin EI share high-affinity potentiation and low-affinity inhibition of nicotinic acetylcholine receptors. FEBS J 2007, 274: 3972-3985.
9. López-Vera E, Jacobsen RB, Ellison M, Olivera BM and Teichert RW. A novel a-conotoxin (a-PIB) isolated from C. purpurascens is selective for skeletal muscle nicotinic acetylcholine receptors. Toxicon 2007, 49: 1193-1199.
10. Yuan DD, Han YH, Wang CG and Chi CW. From the identification of gene organization of a-conotoxins to the cloning of novel toxins. Toxicon 2007, 49: 1135-1149.
11. Shon KJ, Olivera BM, Watkins M, Jacobsen RB, Gray WR, Floresca CZ and Cruz LJ, et al. m-Conotoxin PIIIA, a new peptide for discriminating among tetrodotoxin-sensitive Na channel subtypes. J Neurosci 1998, 18: 4473-4481.
12. Peng C, Han Y, Sanders T, Chew G, Liu J, Hawrot E and Chi C, et al. α4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors. Peptides 2008, 29: 1700-1707.
13. Liu L, Chew G, Hawrot E, Chi C and Wang C. Two potent α3/5 conotoxins from piscivorous Conus achatinus. Acta Biochim Biophys Sin 2007, 39: 438-444.
14. Rosenthal JA, Levandoski MM, Chang B, Potts JF, Shi QL and Hawrot E. The functional role of positively charged amino acid side chains in a-bungarotoxin revealed by site-directed mutagenesis of a His-tagged recombinant a-bungarotoxin. Biochemistry 1999, 38: 7847-7855.
15. Levandoski MM, Lin Y, Moise L, McLaughlin JT, Cooper E and Hawrot E. Chimeric analysis of a neuronal nicotinic acetylcholine receptor reveals amino acids conferring sensitivity to a-bungarotoxin. J Biol Chem 1999, 274: 26113-26119.
16. Nutt LK, Margolis SS, Jensen M, Herman CE, Dunphy WG, Rathmell JC and Kornbluth S. Metabolic regulation of oocyte cell death through the CaMKII-mediated phosphorylation of caspase-2. Cell 2005, 123: 89-103.
17. Sanders T and Hawrot E. A novel pharmatope tag inserted into the b4 subunit confers allosteric modulation to neuronal nicotinic receptors. J Biol Chem 2004, 279: 51460-51465.
18. Gerzanich V, Anand R and Lindstrom J. Homomers of a8 and a7 subunits of nicotinic acetylcholine receptors exhibit similar channel but contrasting binding site properties. Mol Pharmacol 1994, 45: 212-220.
19. Loughnan ML and Alewood PF. Physico-chemical characterization and synthesis of neuronally active a-conotoxins. Eur J Biochem 2004, 271: 2294-2304.
20. Kang TS, Radić Z, Talley TT, Jois SD, Taylor P and Kini RM. Protein folding determinants: structural features determining alternative disulfide pairing in α- and x/conotoxins. Biochemistry 2007, 46: 3338-3355.
21. Favreau P, Krimm I, Le Gall F, Bobenrieth MJ, Lamthanh H, Bouet F and Servent D, et al. Biochemical characterization and nuclear magnetic resonance structure of novel a-conotoxins isolated from the venom of Conus consors. Biochemistry 1999, 38: 6317-6326.
22. Gray WR, Rivier JE, Galyean R, Cruz LJ, Olivera BM and Conotoxin MI. Disulfide bonding and conformational states. J Biol Chem 1983, 258: 12247-12251.
23. Maslennikov IV, Sobol AG, Gladky KV, Lugovskoy AA, Ostrovsky AG, Tsetlin VI and Ivanov VT, et al. Two distinct structures of a-conotoxin GI in aqueous solution. Eur J Biochem 1998, 254: 238-247.
24. Wang CG, Cai Z, Lu W, Wu J, Xu Y, Shi Y and Chi CW. A novel short-chain peptide BmKX from the Chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination. Toxicon 2005, 45: 309-319.
25. Chi SW, Kim DH, Olivera BM, McIntosh JM and Han KH. NMR structure determination of α-conotoxin BuIA, a novel neuronal nicotinic acetylcholine receptor antagonist with an unusual 4/4 disulfide scaffold. Biochem Biophys Res Commun 2006, 349: 1228-1234.