Membrane association of proteins in the aging human lens: Profound changes take place in the fifth decade of life

Investigative Ophthalmology and Visual Science

Volumn 50, Issue 10, 2009, Pages 4786-4793

  Friedrich, Michael G ^a,b   Truscott, Roger J W ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CRYSTALLIN; ANAZOLENE SODIUM; CRYSTALLIN; LENS PROTEIN; PHOSPHOLIPID; MEMBRANE LIPID; MEMBRANE PROTEIN;

AGE DISTRIBUTION; AGING; ARTICLE; CELL MEMBRANE; CELL NUCLEUS; CONTROLLED STUDY; DENSITOMETRY; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; LENS; LIPID ANALYSIS; MALE; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; SUCROSE DENSITY GRADIENT CENTRIFUGATION; ADULT; AGED; DENSITY GRADIENT CENTRIFUGATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LENS NUCLEUS; METABOLISM; MIDDLE AGED; PHYSIOLOGY; TANDEM MASS SPECTROMETRY;

ADULT; AGED; AGED, 80 AND OVER; AGING; CENTRIFUGATION, DENSITY GRADIENT; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRYSTALLINS; DENSITOMETRY; FEMALE; HUMANS; LENS NUCLEUS, CRYSTALLINE; MALE; MEMBRANE LIPIDS; MEMBRANE PROTEINS; MIDDLE AGED; TANDEM MASS SPECTROMETRY; YOUNG ADULT;

EID: 70349578965     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.09-3588     Document Type: Article

References (38)

1. Garland DL, Duglas TY, Jimenez AJ, et al. The nucleus of the human lens: demonstration of a highly characteristic protein pattern by two-dimensional electrophoresis and introduction of a new method of lens dissection. Exp Eye Res. 1996;62:285-291.
2. Kuszak JR. The development of lens sutures. Prog Retin Eye Res. 1995;14:567-591.
3. Lynnerup N, Kjeldsen H, Heegaard S, et al. Radiocarbon dating of the human eye lens crystallines reveal proteins without carbon turnover throughout life. PLoS ONE. 2008;3:e1529.
4. Heys K, Friedrich M, Truscott RJW. Presbyopia and heat: changes associated with aging of the human lens suggest a functional role for the small heat shock protein, alpha-crystallin, in maintaining lens flexibility. Aging Cell. 2007;6:807-815.
5. Heys KR, Friedrich MG, Truscott RJW. Free and bound water in normal and cataractous human lenses. Invest Ophthalmol Vis Sci. 2008;49:1991-1997.
6. Folch-Pi J, Lees M, Stanley G. A simple method for the isolation and purification of total lipides from animal tissue. J Biol Chem. 1957; 226:497-509.
7. Deeley JM, Mitchell TW, Wei X, et al. Human lens lipids differ markedly from those of commonly used experimental animals. Biochim Biophys Acta. 2008;1781:288-298.
8. Chandrasekher G, Cenedella RJ. Protein associated with human lens 'native' membrane during aging and cataract formation. Exp Eye Res. 1995;60:707-717.
9. Schey KL, Data L, Fowler JG, et al. Characterization of human lens major intrinsic protein structure. Invest Ophthalmol Vis Sci. 2000; 41:175-182.
10. Xianlin Han RWG. Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrom Rev. 2005;24:367-412.
11. Broekhuyse RM. Phospholipids in tissues of the eye, III: composition and metabolism of phospholipids in human lens in relation to age and cataract formation. Biochim Biophys Acta. 1969;187:354-365.
12. Ross PL, Huang YN, Marchese JN, et al. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol Cell Proteomics. 2004;3:1154-1169.
13. Spector A. The search for a solution to senile cataracts: Proctor lecture. Invest Ophthalmol Vis Sci. 1984;25:130-146.
14. Spector A, Garner MH, Garner WH, et al. An extrinsic membrane polypeptide associated with high-molecular-weight protein aggregates in human cataract. Science. 1979;204:1323-1326.
15. McHaourab HS, Dodson EK, Koteiche HA. Mechanism of chaperone function in small heat shock proteins: two-mode binding of the excited states of T4 lysozyme mutants by alpha A-crystallin. J Biol Chem. 2002;277:40557-40566.
16. Treweek TM, Lindner RA, Mariani M, et al. The small heat-shock chaperone protein, α-crystallin, does not recognise stable molten globule states of cytosolic proteins. Biochim Biophys Acta. 2000; 1481:175-188.
17. Horwitz J. Alpha-crystallin can function as a molecular chaperone. Proc Natl Acad Sci U S A. 1992;89:10449-10453.
18. Cobb BA, Petrash JM. Alpha-crystallin chaperone-like activity and membrane binding in age-related cataracts. Biochemistry. 2002; 41:483-490.
19. Boyle DL, Takemoto L. EM immunolocalization of alpha-crystallins: association with the plasma membrane from normal and cataractous human lenses. Curr Eye Res. 1996;15:577-582.
20. Roy D, Spector A. Absence of low-molecular-weight alpha crystallin in nuclear region of old human lenses. Proc Natl Acad Sci U S A. 1976;73:3484-3487.
21. McFall-Ngai MJ, Ding L-L, Takemoto LJ, et al. Spatial and temporal mapping of the age-related changes in human lens crystallins. Exp Eye Res. 1985;41:745-758.
22. Rao PV, Huang QL, Horwitz J, et al. Evidence that alpha-crystallin prevents non-specific protein aggregation in the intact eye lens. Biochim Biophys Acta. 1995;1245:439-447.
23. Wilmarth PA, Tanner S, Dasari S, et al. Age-related changes in human crystallins determined from comparative analysis of posttranslational modifications in young and aged lens: does deamidation contribute to crystallin insolubility? J Proteome Res. 2006;5: 2554-2564.
24. Hains PG, Truscott RJW. Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. J Proteome Res. 2007;6:3935-3943.
25. Lampi KJ, Ma Z, Hanson SRA, et al. Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry. Exp Eye Res. 1998;67:31-43.
26. Masters PM, Bada JL, Zigler JS Jr. Aspartic acid racemization in heavy molecular weight crystallins and water insoluble protein from normal human lenses and cataracts. Proc Natl Acad Sci U S A. 1978;75:1204-1208.
27. Fujii N, Ishibashi Y, Satoh K, et al. Simultaneous racemization and isomerization at specific aspartic acid residues in alpha B-crystallin from the aged human lens. Biochim Biophys Acta. 1994;1204: 157-163.
28. Korlimbinis A, Truscott RJW. Identification of 3-hydroxykynurenine bound to proteins in the human lens: a possible role in age-related nuclear cataract. Biochemistry. 2006;45:1950-1960.
29. Cheng R, Feng Q, Argirov OK, et al. Structure elucidation of a novel yellow chromophore from human lens protein. J Biol Chem. 2004;279:45441-45449.
30. Heys KR, Cram SL, Truscott RJW. Massive increase in the stiffness of the human lens nucleus with age: the basis of presbyopia? Mol Vis. 2004;10:956-963.
31. Borchman D, Ozaki Y, Lamba OP, et al. Age and regional structural characterization of lipid hydrocarbon chains from human lenses by infrared, and near-infrared raman, spectroscopies. Biospectroscopy. 1996;2:113-123.
32. Duncan G, van Heyeningen R. Distribution of non-diffusible calcium and sodium in normal and cataractous human lenses. Exp Eye Res. 1977;25:183-193.
33. Duncan G, Hightower KR, Gandolfi SA, et al. Human lens membrane cation permeability increases with age. Invest Ophthalmol Vis Sci. 1989;30:1855-1859.
34. Rajini B, Shridas P, Sundari CS, et al. Calcium binding properties of gamma-crystallin: calcium ion bind at the Greek key beta gammacrystallin fold. J Biol Chem. 2001;276:38464-38471.
35. Giblin FJ, Hightower KR, Ragatzki PA, et al. Calcium-induced high molecular weight proteins in the intact rabbit lens. Exp Eye Res. 1984;39:9-17.
36. Rujoi M, Borchman D, Du Pré DB, et al. Interactions of Ca2+ with sphingomyelin and dihydrosphingomyelin. Biophys J. 2002;82: 3096-3104.
37. Moffat BA, Landman KA, Truscott RJ, et al. Age-related changes in the kinetics of water transport in normal human lenses. Exp Eye Res. 1999;69:663-669.
38. Sweeney MH, Truscott RJ. An impediment to glutathione diffusion in older normal human lenses: a possible precondition for nuclear cataract. Exp Eye Res. 1998;67:587-595.