Extracellular acid protease from Aspergillus niger I1: Purification and characterization

African Journal of Biotechnology

Volumn 8, Issue 18, 2009, Pages 4582-4589

  Siala, Rayda ^a   Sellami Kamoun, Alya ^a   Hajji, Mohamed ^a   Abid, Ines ^a   Gharsallah, Neji ^a   Nasri, Moncef ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Acid protease; Aspergillopepsin; Aspergillus niger; Glycosylation; Purification

Indexed keywords

ACID PROTEINASE; ASPARTIC PROTEINASE; SEPHADEX;

ARTICLE; ASPERGILLUS NIGER; BACTERIAL STRAIN; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; GEL FILTRATION; ION EXCHANGE; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; ULTRAFILTRATION;

ASPERGILLUS NIGER;

EID: 70349515516     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Alessandro M, Federico F (1980). Partial purification and characterization of a yeast extracellular acid protease. J. Dairy Sci. 63: 1397-2402.
2. Basten DEJW, Visser J, Schaap PJ (2001). Lysine aminopeptidase of Aspergillus niger. Microbiology, 147: 2045-2050
3. Bradford M (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 72: 248-254
4. Chrzanowska J, Klaczkowska M, Dryjanski M, Stachowiak D, Polanowski A (1995). Aspartic proteinase from Penicillium camemberti: purification, properties and substrate specificity. Enzyme Microb. Technol. 17: 719-724
5. Dal Degan F, Ribadeau-Dumas B, Breddam K (1992). Purification and characterization of two serine carboxypeptidases from Aspergillus niger and their use in C-terminal sequencing of proteins and peptide synthesis. Appl. Environ. Microbiol. 58: 2144-2152
6. Dara OD, Wang L, Xu J, Darin R, Gu T, Murray MY (2001). Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity. Biochem. Eng. J. 8: 187-193.
7. Dregwood R (1946). Carbohydrate quantification. Ind. Eng. Chem. Analytical Edition 18: 499.
8. Garcia-Carreno FL, Haard NF (1993). Characterization of proteinase classes in Langostilla (Pleuroncodes planipes) and crayfish (Pacifastacus astacus) extracts. J. Food Biochem. 17: 97-113.
9. Gomi K, Arikawa K, Kamiya N, Kitamoto K, Kumagai C (1993). Cloning and nucleotide sequence of the acid protease-encoding gene (pepA) from Aspergillus oryzae. Biosci Biotechnol. Biochem. 57: 1095-1100
10. Gupta R, Beg QK, Lorenz P (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol. 59: 15-32.
11. Hashem AM (1999). Optimization of milk clotting enzyme productivity by Penicillium oxalicum. Biores Technol. 70: 203-207.
12. Hashimoto H, Iwaasa T, Yokotsuka T (1973). Some properties of acid protease from the thermophilic Penicillium duponti K1014. Appl. Microbiol. 25: 578-583.
13. Ichishima E (2004). Aspergillopepsin I. In Handbook of Proteolytic Enzymes, 2 edn (Barrett,A.J., Rawlings,N.D. & Woessner,J.F. eds), pp. 92-99, Elsevier, London.
14. Inoue H, Lu JF, Athauda SBP, Kong KH, Hayashi T, Kimura T, Makabe O, Takahashi K (1995). Aspergillus niger var. macrosporus proteinase B. cDNA cloning, expression and activation of the proenzyme. Adv. Exp. Med. Biol. 362: 581-587.
15. Inoue H, Hayashi T, Huang XP, Lu J, Athanda SBP, Kong K, Yamagata H, Ukada S, Takahashi K (1996). Heterologous expression and site-directed mutagenesis studies on the activation mechanism and the roles of the basic residues in the prosegment of aspergillopepsinogen I. Eur. J. Biochem. 237: 719-725
16. Ishibashi N, Ono I, Kato K, Shigenaga T, Shinoda I, Okai H, Fukui S (1988). Role of the hydrophobic amino acid residue in the bitterness of peptides. Agr. Biol. Chem. 52:91-94
17. Kembhavi AA, Kulharni A, Pant AA (1993). Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM No.64. Appl. Biochem. Biotechnol. 38: 83-92.
18. Kumar S, Sharma NS, Saharan MR, Singh R (2005). Extracellular acid protease from Rhizopus oryzae: purification and characterization. Proc. Biochem. 40: 1701-1705.
19. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
20. Lineweaver H, Burk D (1934). The determination of enzyme dissociation constants. Journal of the American Chemical Society, 56, 665-666.
21. Moralejo FJ, Cardoza RE, Gutierrez S, Lombraña M, Fierro F, Martin JF (2002). Silencing of the aspergillopepsin B gene of Aspergillus awamori by antisense RNA expression of protease removal by gene disruption results in a large increase in Thaumatin production. Appl Environ Microbiol. 68:3550-3559.
22. Morimura S, Kida K, Sonoda Y (1994). Purification of protease using waste water from the manufacture of Shochu. J. Ferm. Bioeng. 77: 183-187.
23. Monod M, Capoccia S, Léchenne B, Zaugg C, Holdom M, Jousson O (2002). Secreted proteases from pathogenic fungi. Int. J. Med. Microbiol. 292: 405-419.
24. Olajuyigbe FM, Ajele JO, Olawoye, TL (2003). Some physicochemical properties of acid protease produced during growth of Aspergillus niger (NRRL 1785). Global J. Pure Appl. Sci. 9: 523-528.
25. Pericin DM, Madarev-Popovic SZ, Radulovic-Popovic LM (2009). Optimization of conditions for acid protease partitioning and purification in aqueous two-phase systems using response surface methodology. Biotechnol. Lett. 31: 43-47.
26. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998). Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 62: 597-635.
27. Reichard U, Monod M, Ruechel R (1995). Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus. FEMS. Microbiol. Lett. 130: 69-74.
28. Somkuti G/A, Babel FJ (1968). Purification and properties of Mucor pusillus acid protease. J. Bacteriol. 95: 1407-1414
29. Sumantha A, Larroche C, Pandey A (2006). Microbiology and industrial biotechnology of food-grade proteases: a perspective. Food Technol. Biotechnol. 44: 211-220.
30. Takahashi K, Inoue H, Sakai K, Kohama T, Kitahara S, Takashima K, Tanji M, Athauda SBP, Takahashi T, Akanuma H, Mamiya G, Yamasaki M (1991). The primary structure of Aspergillus niger acid proteinase A. J Biol Chem. 266: 19480-19483.
31. Tello-Solis SR, Hernandez-Arana A (1995). Effect of irreversibility on the thermodynamic characterization of the thermal denaturation of Aspergillus saitoi acid proteinase. Biochem. J. 311: 969-974
32. Tsujita Y, Endo A (1978). Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae. Eur. J. Biochem. 15: 347-353. (Pubitemid 8301263)
33. Van den Hombergh JP, Van de Vondervoort PJI, Fraissinet-Tachet L, Visser J (1997). Aspergillus as a host for heterologous protein production: the problem of proteases. Trends Biotechnol. 15: 256-263. (Pubitemid 27342095)
34. Ward OP (1983). Proteinases. In Microbiol Enzymes and Biotechnology Fogarty, WM. (Ed.): Applied Science Publishers. New York. 251-305.
35. Wu LC, Hang YD (1998). Purification and characterization of acid proteinase from Neosartorya fischeri var. spinosa IBT 4872. Lett. Appl. Microbiol. 27: 71-75.
36. Zhu H, Guo DC, Dancik BP (1990). Purification and characterization of an extracellular acid proteinase from the ectomycorrhizal fungus Hebeloma crustuliniforme. Appl. Environ. Microbiol. 56: 837-843. (Pubitemid 20114430)