Biochemical analysis of the human EVL domains in homologous recombination

FEBS Journal

Volumn 276, Issue 20, 2009, Pages 5841-5848

  Takaku, Motoki ^a   MacHida, Shinichi ^a   Nakayama, Shugo ^a   Takahashi, Daisuke ^a   Kurumizaka, Hitoshi ^a  

^a WASEDA UNIVERSITY   (Japan)

Author keywords

DSB; ENA VASP; EVL; Homologous recombination; RAD51

Indexed keywords

DNA; ENABLED VASODILATOR STIMULATED PHOSPHOPROTEIN; RAD51 PROTEIN; UNCLASSIFIED DRUG; VASODILATOR STIMULATED PHOSPHOPROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL ANALYSIS; CONTROLLED STUDY; DNA BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PURIFICATION;

CELL ADHESION MOLECULES; HUMANS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RAD51 RECOMBINASE; RECOMBINATION, GENETIC;

EID: 70349496097     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.07265.x     Document Type: Article

References (30)

1. Agarwal S, Tafel AA Kanaar R (2006) DNA double-strand break repair and chromosome translocations. DNA Repair (Amst) 5, 1075 1081. (Pubitemid 44291620)
2. Weinstock DM, Richardson CA, Elliott B Jasin M (2006) Modeling oncogenic translocations: distinct roles for double-strand break repair pathways in translocation formation in mammalian cells. DNA Repair (Amst) 5, 1065 1074.
3. Wyman C Kanaar R (2006) DNA double-strand break repair: all's well that ends well. Annu Rev Genet 40, 363 383. (Pubitemid 44956790)
4. West SC (2003) Molecular views of recombination proteins and their control. Nat Rev Mol Cell Biol 4, 435 445.
5. Symington LS (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66, 630 670.
6. Sung P, Krejci L, Van Komen S Sehorn MG (2003) Rad51 recombinase and recombination mediators. J Biol Chem 278, 42729 42732.
7. San Filippo J, Sung P Klein H (2008) Mechanism of eukaryotic homologous recombination. Annu Rev Biochem 77, 229 257.
8. Modesti M, Budzowska M, Baldeyron C, Demmers JA, Ghirlando R Kanaar R (2007) RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination. Mol Cell 28, 468 481.
9. Wiese C, Dray E, Groesser T, San Filippo J, Shi I, Collins DW, Tsai MS, Williams GJ, Rydberg B, Sung P et al. (2007) Promotion of homologous recombination and genomic stability by RAD51AP1 via RAD51 recombinase enhancement. Mol Cell 28, 482 490.
10. Morozumi Y, Takizawa Y, Takaku M Kurumizaka H (2009) Human PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities. Nucleic Acids Res 37, 4296 4307.
11. Takaku M, Machida S, Hosoya N, Nakayama S, Takizawa Y, Sakane I, Shibata T, Miyagawa K Kurumizaka H (2009) Recombination activator function of the novel RAD51- and RAD51B-binding protein, human EVL. J Biol Chem 284, 14326 14336.
12. Kwiatkowski AV, Gertler FB Loureiro JJ (2003) Function and regulation of Ena/VASP proteins. Trends Cell Biol 13, 386 392.
13. Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC Gygi SP (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci USA 101, 12130 12135.
14. Takata M, Sasaki MS, Sonoda E, Morrison C, Hishimoto M, Utsumi H, Yamaguchi-Iwai Y, Shinohara A Takeda S (1998) Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J 17, 5497 5508.
15. Hüttelmaier S, Harbeck B, Steffens O, Messerschmidt T, Illenberger S Jockusch BM (1999) Characterization of the actin binding properties of the vasodilator-stimulated phosphoprotein VASP. FEBS Lett 451, 68 74.
16. Walders-Harbeck B, Khaitlina SY, Hinssen H, Jockusch BM Illenberger S (2002) The vasodilator-stimulated phosphoprotein promotes actin polymerisation through direct binding to monomeric actin. FEBS Lett 529, 275 280.
17. Krause M, Dent EW, Bear JE, Loureiro JJ Gertler FB (2003) Ena/VASP proteins: regulators of the actin cytoskeleton and cell migration. Annu Rev Cell Dev Biol 19, 541 564.
18. Barzik M, Kotova TI, Higgs HN, Hazelwood L, Hanein D, Gertler FB Schafer DA (2005) Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J Biol Chem 280, 28653 28662.
19. Loureiro JJ, Rubinson DA, Bear JE, Baltus GA, Kwiatkowski AV Gertler FB (2002) Critical roles of phosphorylation and actin binding motifs, but not the central proline-rich region, for Ena/vasodilator-stimulated phosphoprotein (VASP) function during cell migration. Mol Biol Cell 13, 2533 2546.
20. Applewhite DA, Barzik M, Kojima S, Svitkina TM, Gertler FB Borisy GG (2007) Ena/VASP proteins have an anti-capping independent function in filopodia formation. Mol Biol Cell 18, 2579 2591.
21. Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl UD, Walter U, Gertler FB, Wehland J Chakraborty T (1997) A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J 16, 5433 5444.
22. Carl UD, Pollmann M, Orr E, Gertlere FB, Chakraborty T Wehland J (1999) Aromatic and basic residues within the EVH1 domain of VASP specify its interaction with proline-rich ligands. Curr Biol 9, 715 718.
23. Fedorov AA, Fedorov E, Gertler F Almo SC (1999) Structure of EVH1, a novel proline-rich ligand-binding module involved in cytoskeletal dynamics and neural function. Nat Struct Biol 6, 661 665.
24. Gertler FB, Comer AR, Juang JL, Ahern SM, Clark MJ, Liebl EC Hoffmann FM (1995) Enabled, a dosage-sensitive suppressor of mutations in the Drosophila Abl tyrosine kinase, encodes an Abl substrate with SH3 domain-binding properties. Genes Dev 9, 521 533.
25. Ermekova KS, Zambrano N, Linn H, Minopoli G, Gertler F, Russo T Sudol M (1997) The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila enabled. J Biol Chem 272, 32869 32877.
26. Ahern-Djamali SM, Bachmann C, Hua P, Reddy SK, Kastenmeier AS, Walter U Hoffmann FM (1999) Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc Natl Acad Sci USA 96, 4977 4982.
27. Kurumizaka H, Aihara H, Kagawa W, Shibata T Yokoyama S (1999) Human Rad51 amino acid residues required for Rad52 binding. J Mol Biol 291, 537 548.
28. Matsuo Y, Sakane I, Takizawa Y, Takahashi M Kurumizaka H (2006) Roles of the human Rad51 L1 and L2 loops in DNA binding. FEBS J 273, 3148 3159.
29. Ishida T, Takizawa Y, Sakane I Kurumizaka H (2008) The Lys313 residue of the human Rad51 protein negatively regulates the strand-exchange activity. Genes Cells 13, 91 103.
30. Kagawa W, Kurumizaka H, Ikawa S, Yokoyama S Shibata T (2001) Homologous pairing promoted by the human Rad52 protein. J Biol Chem 276, 35201 35208.