Critical roles of phosphorylation and actin binding motifs, but not the central proline-rich region, for Ena/vasodilator-stimulated phosphoprotein (VASP) function during cell migration

Molecular Biology of the Cell

Volumn 13, Issue 7, 2002, Pages 2533-2546

  Loureiro, Joseph J ^a   Rubinson, Douglas A ^a   Bear, James E ^a   Baltus, Gretchen A ^a   Kwiatkowski, Adam V ^a   Gertler, Frank B ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PROLINE; VASODILATOR STIMULATED PHOSPHOPROTEIN; ACTIN; ACTIN BINDING PROTEIN; CARRIER PROTEIN; CELL ADHESION MOLECULE; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYTOSKELETON PROTEIN; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; ENAH PROTEIN, MOUSE; HYBRID PROTEIN; N WASP PROTEIN, RAT; N-WASP PROTEIN, RAT; NERVE PROTEIN; NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; PHOSPHOPROTEIN; PHOTOPROTEIN; SERINE; THREONINE; VASODILATOR-STIMULATED PHOSPHOPROTEIN; VINCULIN; WASL PROTEIN, MOUSE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL MIGRATION; CELL MOTILITY; FLUORESCENCE ACTIVATED CELL SORTING; IMMUNOFLUORESCENCE; MUTATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; RAT; REGULATORY MECHANISM; ANIMAL; BINDING SITE; CELL CULTURE; CELL MOTION; CHEMISTRY; CYTOLOGY; FIBROBLAST; GENETIC COMPLEMENTATION; GENETICS; METABOLISM; MOUSE; PHOSPHORYLATION; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; REPORTER GENE;

ACTINS; AMINO ACID MOTIFS; ANIMALS; BINDING SITES; CARRIER PROTEINS; CELL ADHESION MOLECULES; CELL MOVEMENT; CELLS, CULTURED; CYCLIC AMP-DEPENDENT PROTEIN KINASES; CYTOSKELETAL PROTEINS; FIBROBLASTS; GENES, REPORTER; GENETIC COMPLEMENTATION TEST; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICE; MICROFILAMENT PROTEINS; NERVE TISSUE PROTEINS; PHENOTYPE; PHOSPHOPROTEINS; PHOSPHORYLATION; PROLINE; PROTEIN STRUCTURE, TERTIARY; RATS; RECOMBINANT FUSION PROTEINS; SERINE; THREONINE; VINCULIN; WISKOTT-ALDRICH SYNDROME PROTEIN, NEURONAL;

EID: 0036323119     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E01-10-0102     Document Type: Article

References (38)

1. Mutations in Drosophila enabled and rescue by human vasodilator-stimulated phosphoprotein (VASP) indicate important functional roles for Ena/VASP homology domain 1 (EVH1) and EVH2 domains
2. The vasodilator-stimulated phosphoprotein (VASP) is involved in cGMP- and cAMP-mediated inhibition of agonist-induced platelet aggregation, but is dispensable for smooth muscle function
3. The EVH2 domain of the vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and actin bundle formation
4. Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor
5. Regulating cellular actin assembly
6. Negative regulation of fibroblast motility by Ena/VASP proteins
7. Antagonism between Ena/VASP Proteins and Actin Filament Capping Regulaties Fibroblast Motility
8. cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets
9. Aromatic and basic residues within the EVH1 domain of VASP specify its interaction with proline-rich ligands
10. AKAP signaling complexes at the cytoskeleton
11. The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila enabled
12. Structure of EVH1, a novel proline-rich ligand-binding module involved in cytoskeletal dynamics and neural function
13. Accumulation of profilin II at the surface of Listeria is concomitant with the onset of motility and correlates with bacterial speed
14. Enabled, a dosage-sensitive suppressor of mutations in the Drosophila Abl tyrosine kinase, encodes an Abl substrate with SH3 domain-binding properties
15. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
16. Stoichiometric and reversible phosphorylation of a 46-kDa protein in human platelets in response to cGMP- and cAMP-elevating vasodilators
17. Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin
18. Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice
19. Characterization of the actin binding properties of the vasodilator-stimulated phosphoprotein VASP
20. Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton
21. Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex
22. cAMP-dependent protein kinase phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3 domains
23. Mena is required for neurulation and commissure formation
24. From Abl to actin: Abl tyrosine kinase and associated proteins in growth cone motility
25. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
26. N-WASP, WAVE and Mena play different roles in the organization of actin cytoskeleton in lamellipodia
27. A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family
28. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells
29. Structure of the enabled/VASP homology 1 domain-peptide complex: A key component in the spatial control of actin assembly
30. Vasodilator-stimulated phosphoprotein is involved in stress-fiber and membrane ruffle formation in endothelial cells
31. The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins
32. The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts
33. VASP dynamics during lamellipodia protrusion
34. The Alzheimer amyloid precursor protein (APP) and FE65, an APP-binding protein, regulate cell movement
35. Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility
36. The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin
37. The actin binding site of thymosin beta 4 mapped by mutational analysis
38. Role of cyclic nucleotide-dependent protein kinases and their common substrate VASP in the regulation of human platelets