메뉴 건너뛰기




Volumn 8, Issue 3, 2009, Pages 214-225

Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; MEX67 PROTEIN, S CEREVISIAE; NUCLEAR PROTEIN; NUCLEOCYTOPLASMIC TRANSPORT PROTEIN; NUCLEOPORIN; NUP1 PROTEIN, S CEREVISIAE; RECOMBINANT PROTEIN; RNA BINDING PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

EID: 70349453984     PISSN: None     EISSN: 19317913     Source Type: Journal    
DOI: 10.1187/cbe.09-03-0019     Document Type: Article
Times cited : (5)

References (38)
  • 2
    • 0028064385 scopus 로고
    • Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p
    • Belanger, K. D., Kenna, M. A., Wei, S., and Davis, L. I. (1994). Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. J. Cell Biol. 126, 619-630.
    • (1994) J. Cell Biol. , vol.126 , pp. 619-630
    • Belanger, K.D.1    Kenna, M.A.2    Wei, S.3    Davis, L.I.4
  • 3
    • 0027944460 scopus 로고
    • Nup1 mutants exhibit pleiotropic defects in nuclear pore complex function
    • Bogerd A. M., Hoffman, J. A., Amberg, D. C., Fink, G. R., and Davis, L. I. (1994). nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. J. Cell Biol. 127, 319-332.
    • (1994) J. Cell Biol. , vol.127 , pp. 319-332
    • Bogerd, A.M.1    Hoffman, J.A.2    Amberg, D.C.3    Fink, G.R.4    Davis, L.I.5
  • 4
    • 0033527749 scopus 로고    scopus 로고
    • The yeast nucleoporin Nup2p is involved in nuclear export of importin alpha/Srp1p
    • Booth, J. W., Belanger, K. D., Sannella, M. I., and Davis, L. I. (1999). The yeast nucleoporin Nup2p is involved in nuclear export of importin alpha/Srp1p. J. Biol. Chem. 274, 32360-32367.
    • (1999) J. Biol. Chem. , vol.274 , pp. 32360-32367
    • Booth, J.W.1    Belanger, K.D.2    Sannella, M.I.3    Davis, L.I.4
  • 6
    • 70349468345 scopus 로고    scopus 로고
    • Assessment results following inquiry and traditional physics laboratory activities
    • Bryan, J. A. (2006). Assessment results following inquiry and traditional physics laboratory activities. J. Coll. Sci. Teach. 35, 56-61.
    • (2006) J. Coll. Sci. Teach. , vol.35 , pp. 56-61
    • Bryan, J.A.1
  • 8
    • 0025367662 scopus 로고
    • The NUP1 gene encodes an essential component of the yeast nuclear pore complex
    • Davis, L. I., and Fink, G. R. (1990). The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61, 965-978.
    • (1990) Cell , vol.61 , pp. 965-978
    • Davis, L.I.1    Fink, G.R.2
  • 9
    • 18744370194 scopus 로고    scopus 로고
    • The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
    • Fischer, T., Strässer, K., Rácz, A., Rodriguez-Navarro, S., Oppizzi, M., Ihrig, P., Lechner, J., and Hurt, E. (2002). The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. EMBO J. 21, 5843-5852.
    • (2002) EMBO J. , vol.21 , pp. 5843-5852
    • Fischer, T.1    Strässer, K.2    Rácz, A.3    Rodriguez-Navarro, S.4    Oppizzi, M.5    Ihrig, P.6    Lechner, J.7    Hurt, E.8
  • 10
    • 70349458304 scopus 로고    scopus 로고
    • Was "inquiry" a mistake? It's all in the name
    • French, D. P. (2005). Was "inquiry" a mistake? It's all in the name. J. Coll. Sci. Teach. 35, 60.
    • (2005) J. Coll. Sci. Teach. , vol.35 , pp. 60
    • French, D.P.1
  • 11
    • 17944403557 scopus 로고    scopus 로고
    • Characterization of pathogenic human MSH2 missense mutations using yeast as a model system: A laboratory course in molecular biology
    • Gammie, A. E., and Erdeniz, N. (2004). Characterization of pathogenic human MSH2 missense mutations using yeast as a model system: a laboratory course in molecular biology. Cell Biol. Educ. 3, 31-48.
    • (2004) Cell Biol. Educ. , vol.3 , pp. 31-48
    • Gammie, A.E.1    Erdeniz, N.2
  • 12
    • 0036270543 scopus 로고    scopus 로고
    • Transformation of yeast by the LiAc/SS carrier DNA/PEG method
    • Gietz, R. D., and Woods, R. A. (2002). Transformation of yeast by the LiAc/SS carrier DNA/PEG method. Methods Enzymol 350, 87-96.
    • (2002) Methods Enzymol , vol.350 , pp. 87-96
    • Gietz, R.D.1    Woods, R.A.2
  • 15
    • 0027937653 scopus 로고
    • Isolation of a protein that is essential for the first step of nuclear protein import
    • Gorlich, D., Prehn, S., Laskey, R. A., and Hartmann, G. (1994). Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79, 767-778.
    • (1994) Cell , vol.79 , pp. 767-778
    • Gorlich, D.1    Prehn, S.2    Laskey, R.A.3    Hartmann, G.4
  • 18
    • 24344495278 scopus 로고    scopus 로고
    • Using a module-based laboratory to incorporate inquiry into a large cell biology course
    • Howard, D. R., and Miskowski, J. A. (2005). Using a module-based laboratory to incorporate inquiry into a large cell biology course. Cell Biol. Educ. 4, 249-260.
    • (2005) Cell Biol. Educ. , vol.4 , pp. 249-260
    • Howard, D.R.1    Miskowski, J.A.2
  • 19
    • 34648816826 scopus 로고    scopus 로고
    • Exporting RNA from the nucleus to the cytoplasm
    • Kohler, A., and Hurt, E. (2007). Exporting RNA from the nucleus to the cytoplasm. Nat. Rev. Mol. Cell Biol. 8, 761-773.
    • (2007) Nat. Rev. Mol. Cell Biol. , vol.8 , pp. 761-773
    • Kohler, A.1    Hurt, E.2
  • 20
    • 70349447091 scopus 로고    scopus 로고
    • Putting inquiry teaching to the test: Enhancing learning in college botany
    • Lord, T., Chad, S., and Zimmerman, R. (2007). Putting inquiry teaching to the test: enhancing learning in college botany. J. Coll. Sci. Teach. 36, 62-65.
    • (2007) J. Coll. Sci. Teach. , vol.36 , pp. 62-65
    • Lord, T.1    Chad, S.2    Zimmerman, R.3
  • 21
    • 38349097688 scopus 로고    scopus 로고
    • Using Saccharomyces cerevisiae to test the mutagenicity of household compounds: An open ended hypothesis-driven teaching lab
    • Marshall, P. A. (2007). Using Saccharomyces cerevisiae to test the mutagenicity of household compounds: an open ended hypothesis-driven teaching lab. CBE Life Sci. Educ. 6, 307-315.
    • (2007) CBE Life Sci. Educ. , vol.6 , pp. 307-315
    • Marshall, P.A.1
  • 22
    • 0030593468 scopus 로고    scopus 로고
    • Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels
    • Miroux, B., and Walker, J. E. (1996). Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260, 289-298.
    • (1996) J. Mol. Biol. , vol.260 , pp. 289-298
    • Miroux, B.1    Walker, J.E.2
  • 23
    • 33750063175 scopus 로고    scopus 로고
    • From organelle to protein gel: A 6-wk laboratory project on flagellar proteins
    • Mitchell, B. F., and Graziano, M. R. (2006). From organelle to protein gel: a 6-wk laboratory project on flagellar proteins. CBE Life Sci. Educ. 5, 239-246.
    • (2006) CBE Life Sci. Educ. , vol.5 , pp. 239-246
    • Mitchell, B.F.1    Graziano, M.R.2
  • 24
    • 0003608696 scopus 로고    scopus 로고
    • National Research Council (NRC), Washington, DC: National Academies Press
    • National Research Council (NRC) (1996). National Science Education Standards, Washington, DC: National Academies Press.
    • (1996) National Science Education Standards
  • 27
    • 0346124060 scopus 로고    scopus 로고
    • National Science Foundation, Washington, DC: National Science Foundation
    • National Science Foundation (1996). Shaping the Future, Washington, DC: National Science Foundation.
    • (1996) Shaping the Future
  • 28
    • 0037931430 scopus 로고    scopus 로고
    • National Science Teachers Association, Arlington, VA: National Science Teachers Association Press
    • National Science Teachers Association (2001). Practicing Science: The Investigative Approach in College Science Teaching, Arlington, VA: National Science Teachers Association Press.
    • (2001) Practicing Science: The Investigative Approach in College Science Teaching
  • 29
    • 0642311319 scopus 로고    scopus 로고
    • Using the two-hybrid screen in the classroom laboratory
    • Odom, D. P., and Grossel, M. J. (2002). Using the two-hybrid screen in the classroom laboratory. CBE Life Sci. Educ. 1, 43-62.
    • (2002) CBE Life Sci. Educ. , vol.1 , pp. 43-62
    • Odom, D.P.1    Grossel, M.J.2
  • 30
    • 33645619444 scopus 로고    scopus 로고
    • Implementing inquiry-guided instruction: Practical issues
    • Oliver-Hoyo, M. T., Allen, D., and Anderson, M. A. (2004). Implementing inquiry-guided instruction: practical issues. J. Coll. Sci. Teach. 33, 20-24.
    • (2004) J. Coll. Sci. Teach. , vol.33 , pp. 20-24
    • Oliver-Hoyo, M.T.1    Allen, D.2    Anderson, M.A.3
  • 31
    • 34548608900 scopus 로고    scopus 로고
    • The many faces of inductive teaching and learning
    • Prince, M., and Felder, R. M. (2007). The many faces of inductive teaching and learning. J. Coll. Sci. Teach. 36, 14-20.
    • (2007) J. Coll. Sci. Teach. , vol.36 , pp. 14-20
    • Prince, M.1    Felder, R.M.2
  • 32
    • 0034695924 scopus 로고    scopus 로고
    • The yeast nuclear pore complex: Composition, architecture, and transport mechanism
    • Rout, M. P., Aitchison, J. D., Suprapto, A., Hjertaas, K., Zhao, Y., and Chait, B. T. (2000). The yeast nuclear pore complex: composition, architecture, and transport mechanism. J. Cell Biol. 148, 635-651.
    • (2000) J. Cell Biol. , vol.148 , pp. 635-651
    • Rout, M.P.1    Aitchison, J.D.2    Suprapto, A.3    Hjertaas, K.4    Zhao, Y.5    Chait, B.T.6
  • 33
    • 0036571478 scopus 로고    scopus 로고
    • Performance of students in project-based science classrooms on a national measure of science achievement
    • Schneider, R. M., Krajcik, J., Marx, R. W., and Soloway, E. (2002). Performance of students in project-based science classrooms on a national measure of science achievement. J Res. Sci. Teach. 39, 410-422.
    • (2002) J Res. Sci. Teach. , vol.39 , pp. 410-422
    • Schneider, R.M.1    Krajcik, J.2    Marx, R.W.3    Soloway, E.4
  • 34
    • 0030911425 scopus 로고    scopus 로고
    • Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A) RNA and nuclear pores
    • Segref, A., Sharma, K., Doye, V., Hellwig, A., Huber, J., Luhrmann, R., and Hurt, E. (1997). Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A) RNA and nuclear pores. EMBO J. 16, 3256-3271.
    • (1997) EMBO J , vol.16 , pp. 3256-3271
    • Segref, A.1    Sharma, K.2    Doye, V.3    Hellwig, A.4    Huber, J.5    Luhrmann, R.6    Hurt, E.7
  • 35
    • 0034698683 scopus 로고    scopus 로고
    • Binding of the Mex67p/ Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
    • Strasser, K., Bassler, J., and Hurt, E. (2000). Binding of the Mex67p/ Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export. J. Cell Biol. 150, 695-706.
    • (2000) J. Cell Biol. , vol.150 , pp. 695-706
    • Strasser, K.1    Bassler, J.2    Hurt, E.3
  • 36
    • 0035794237 scopus 로고    scopus 로고
    • The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex
    • Strawn, L. A., Shen, T., and Wente, S. R. (2001). The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J. Biol. Chem. 276, 6445-6452.
    • (2001) J. Biol. Chem. , vol.276 , pp. 6445-6452
    • Strawn, L.A.1    Shen, T.2    Wente, S.R.3
  • 37
    • 36749081534 scopus 로고    scopus 로고
    • Crossing the nuclear envelope: Hierarchical regulation of nucleocytoplasmic transport
    • Terry, L. J., Shows, E. B., and Wente, S. R. (2007). Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transport. Science 318, 1412-1416.
    • (2007) Science , vol.318 , pp. 1412-1416
    • Terry, L.J.1    Shows, E.B.2    Wente, S.R.3
  • 38
    • 3242739262 scopus 로고    scopus 로고
    • Analysis of protein localization and secretory pathway function using the yeast Saccharomyces cerevisiae
    • Vallen, E. (2002). Analysis of protein localization and secretory pathway function using the yeast Saccharomyces cerevisiae. Cell Biol. Educ. 1, 173-192.
    • (2002) Cell Biol. Educ. , vol.1 , pp. 173-192
    • Vallen, E.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.