Characterization and gene cloning of a novel serine protease with nematicidal activity from trichoderma pseudokoningii SMF2: RESEARCH LETTER

FEMS Microbiology Letters

Volumn 299, Issue 2, 2009, Pages 135-142

  Chen, Lei Lei ^a   Liu, Li Jun ^a   Shi, Mei ^a   Song, Xiao Yan ^a   Zheng, Chang Ying ^a   Chen, Xiu Lan ^a   Zhang, Yu Zhong ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Characterization; Gene clone; Meloidogyne incognita; Nematicidal activity; Serine protease; Trichoderma pseudokoningii SMF2

Indexed keywords

ANTINEMATODAL AGENT; BOVINE SERUM ALBUMIN; GELATIN; LYSOZYME; SERINE PROTEINASE; SERINE PROTEINASE SPRT; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; MELOIDOGYNE INCOGNITA; MOLECULAR CLONING; MOLECULAR WEIGHT; NEMATODE; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; THERMOSTABILITY; TRICHODERMA; TRICHODERMA PSEUDOKONINGII;

ANIMALS; ANTINEMATODAL AGENTS; CLONING, MOLECULAR; ENZYME ACTIVATORS; ENZYME STABILITY; GELATIN; HYDROGEN-ION CONCENTRATION; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MURAMIDASE; PHYLOGENY; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SERINE ENDOPEPTIDASES; SERUM ALBUMIN, BOVINE; SURVIVAL ANALYSIS; TEMPERATURE; TRICHODERMA; TYLENCHOIDEA;

BOVINAE; FUNGI; HYPOCREA PSEUDOKONINGII; MELOIDOGYNE INCOGNITA; NEMATODA; TRICHODERMA;

EID: 70349245249     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01746.x     Document Type: Article

References (33)

1. Bonants PJ, Fitters PF, Thijs H, den Belder E, Waalwijk C Henfling JW (1995) A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs. Microbiology 141 : 775 784.
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 : 248 254.
3. Casas-Flores S Herrera-Estrella A (2007) Antagonism of plant parasitic nematodes by fungi. The Mycota (Kubicek CP Druzhinina IS, eds), pp. 147 157. Springer, Berlin.
4. Chen X, Zhang Y, Gao P Luan X (2003) Two different proteases produced by a deep-sea psychrotrophic bacterial strain, Pseudoaltermonas sp. SM9913. Mar Biol 143 : 989 993.
5. Emanuelsson O, Brunak S, Gunnar von H Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP, and related tools. Nat Protoc 2 : 953 971.
6. Geremia RA, Goldman GH, Jacobs D, Ardiles W, Vila SB, Van Montagu M Herrera-Estrella A (1993) Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum. Mol Microbiol 8 : 603 613.
7. Harman GE (2006) Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96 : 190 194.
8. Huang Q, Tezuka Y, Kikuchi T, Nishi A, Tubaki K Tanaka K (1995) Studies on metabolites of mycoparasitic fungi. II. Metabolites of Trichoderma koningii. Chem Pharm Bull 43 : 223 229.
9. Khan A, Williams KL Nevalainen HKM (2004) Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles. Biol Control 31 : 346 352.
10. Khan TA Saxena SK (1997) Effect of root-dip treatment with fungal filtrates on root penetration, development and reproduction of Meloidogyne javanica on tomato. Int J Nematol 7 : 85 88.
11. Kredics L, Antal Z, Szekeres A, Hatvani L, Manczinger L, Vagvolgyi C Nagy E (2005) Extracellular proteases of Trichoderma species. Acta Microbiol Immunol Hung 52 : 169 184.
12. Kumar S, Tamura K Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5 : 150 163.
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
14. Marchler-Bauer A, Anderson JB, Derbyshire MK et al 2007) CDD: a conserved domain database for interactive domain family analysis. Nucleic Acids Res 35 : D237 D240.
15. Meyer SLF, Massoud SI, Chitwood DJ Roberts DP (2000) Evaluation of Trichoderma virens and Bulkholderia cepacia for antagonistic activity against root-knot nematode, Meloidogyne incognita. Nematology 2 : 871 879.
16. Morton CO, Hirsch PR, Peberdy JP Kerry BR (2003) Cloning of and genetic variation in protease VCP1 from the nematophagous fungus Pochonia chlamydosporia. Mycol Res 107 : 38 46.
17. Peek K, Veitch DP, Prescott M, Daniel RM, MacIver B Bergquist PL (1993) Some characteristics of a proteinase from a thermophilic Bacillus sp. expressed in Escherichia coli: comparison with the native enzyme and its processing in E. coli and in vitro. Appl Environ Microb 59 : 1168 1175.
18. Pozo MJ, Baek JM, Garcia JM Kenerley CM (2004) Functional analysis of tvsp1, a serine protease-encoding gene in the biocontrol agent Trichoderma virens. Fungal Genet Biol 41 : 336 348.
19. Rao MS, Reddy PP Nagesh M (1998) Evaluation of plant based formulations of Trichoderma harzianum for the management of Meloidogyne incognita on egg plant. Nematologia Mediterranea 26 : 59 62.
20. Segers R, Butt TM, Kerry BR Peberdy JF (1994) The nematophagous fungus Verticillium chlamydosporium produces a chymoelastase-like protease which hydrolyses host nematode proteins in situ. Microbiology 140 : 2715 2723.
21. Sharon ECI, Bar-Eyal M, Chet I, Herrera-Estrella A, Kleifeld O Spiegel Y (2001) Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathology 91 : 687 693.
22. Sharon ECI, Viterbo A, Bar-Eyal M, Nagan H, Samuels GJ Spiegel Y (2007) Parasitism of Trichoderma on Meloidogyne javanica and role of the gelatinous matrix. Eur J Plant Pathol 118 : 247 258.
23. Siddiqui ZA Mahmood I (1996) Biological control of plant parasitic nematodes by fungi: a review. Bioresource Technol 58 : 229 239.
24. Song X, Shen Q, Xie S, Chen X, Sun C Zhang Y (2006) Broad-spectrum antimicrobial activity and high stability of Trichokonins from Trichoderma koningii SMF2 against plant pathogens. FEMS Microbiol Lett 260 : 119 125.
25. Spiegel Y Chet I (1998) Evaluation of Trichoderma spp. as a biocontrol agent against soilborne fungi and plant-parasitic nematodes in Israel. 3 : 169 494.
26. Spiegel Y, Sharon E, Bar-Eyal M, Maghodia A, Vanachter A, Van Assche A, Van Kerckhove S, Viterbo A Chet I (2007) Evaluation and mode of action of Trichoderma isolates as biocontrol agents against plant-parasitic nematodes. Proceedings of IOBC/WPRS Meeting, Spa, Belgium, IOBC/WPRS Bulletin, 30 : 129 133.
27. St Leger RJ, Frank DC, Roberts DW Staples RC (1992) Molecular cloning and regulatory analysis of the cuticle-degrading-protease structural gene from the entomopathogenic fungus Metarhizium anisopliae. Eur J Biochem 204 : 991 1001.
28. Suarez B, Rey M, Castillo P, Monte E Llobell A (2004) Isolation and characterization of PRA1, a trypsin-like protease from the biocontrol agent Trichoderma harzianum CECT 2413 displaying nematicidal activity. Appl Microbiol Biot 65 : 46 55.
29. Sun MH, Gao L, Shi YX, Li BJ Liu XZ (2006) Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. J Invertebr Pathol 93 : 22 28.
30. Tikhonov VE, Lopez-Llorca LV, Salinas J Jansson HB (2002) Purification and characterization of chitinases from the nematophagous fungi Verticillium chlamydosporium and V. suchlasporium. Fungal Genet Biol 35 : 67 78.
31. Windham GL, Windham MT Pederson GA (1993) Interaction of Trichoderma harzianum, Meloidogyne incognita, and Meloidogyne arenaria on Trifolium repens. Nematropica 23 : 99 103.
32. Yang J, Huang X, Tian B, Sun H, Duan J, Wu W Zhang K (2005) Characterization of an extracellular serine protease gene from the nematophagous fungus Lecanicillium psalliotae. Biotechnol Lett 27 : 1329 1334. (Pubitemid 41430459)
33. Yang J, Tian B, Liang L Zhang KQ (2007) Extracellular enzymes and the pathogenesis of nematophagous fungi. Appl Microbiol Biot 75 : 21 31.