Functional analysis of tvsp1, a serine protease-encoding gene in the biocontrol agent Trichoderma virens

Fungal Genetics and Biology

Volumn 41, Issue 3, 2004, Pages 336-348

  Pozo, María J ^a   Baek, Jong Min ^a   García, Juan M ^a   Kenerley, Charles M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Biocontrol; Gene deletion; Gene overexpression; Hydrolytic enzymes; Mycoparasitism; Phytoalexins; Serine protease; Trichoderma virens

Indexed keywords

FUNGAL PROTEIN; PHYTOALEXIN; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; SERINE PROTEINASE;

ANTIMICROBIAL ACTIVITY; ARTICLE; BIOACCUMULATION; BIOLOGICAL PEST CONTROL; CONIDIUM; COTTON; ESCHERICHIA COLI; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE DELETION; GENE EXPRESSION; GENE FUNCTION; GENE OVEREXPRESSION; GENETIC CODE; GROWTH RATE; HYPHOMYCETES; MOLECULAR CLONING; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT DISEASE; PLANT SEED; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; TRICHODERMA; TRICHODERMA VIRENS;

ESCHERICHIA COLI; FUNGI; GOSSYPIUM HIRSUTUM; HYPOCREA VIRENS; RHIZOCTONIA; THANATEPHORUS CUCUMERIS; TRICHODERMA; TRICHODERMA VIRENS;

EID: 0842283307     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fgb.2003.11.002     Document Type: Article

References (34)

1. Ahman J., Johansson T., Olsson M., Punt P.J., van den Hondel C.A.M.J.J., Tunlid A. Improving the pathogenicity of a nematode-trapping fungus by genetic engineering of a subtilisin with nematotoxic activity. Appl. Environ. Microbiol. 68:2002;3408-3415.
2. Baek J.M., Howell C.R., Kenerley C.M. The role of an extracellular chitinase from Trichoderma virens Gv 29-8 in the biocontrol of Rhizoctonia solani. Curr. Genet. 35:1999;41-50.
3. Baek J.M., Kenerley C.M. The arg2 gene of Trichoderma virens: cloning and development of a homologous transformation system. Fungal Genet. Biol. 23:1998;34-44.
4. Belanger R.R., Dufour N., Caron J., Benhamou N. Chronological events associated with the antagonistic properties of Trichoderma harzianum against Botrytis cinera: indirect evidence for sequencial role of antibiosis and parasitism. Biocont. Sci. Technol. 5:1995;41-53.
5. Bonants P.J., Fitters P.F., Thijs H., den Belder E., Waalwijk C., Henfling J.W. A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs. Microbiology. 141:1995;775-784.
6. Chet I., Chernin L. Biocontrol, microbial agents in soil. Bitton G. Encyclopedia of Environmental Microbiology. vol. 1:2002;450-465 Wiley, NY.
7. Cortés C., Gutierrez A., Olmedo V., Inbar J., Chet I., Herrera-Estrella A. The expression of genes involved in parasitism by Trichoderma harzianum is triggered by a diffusible factor. Mol. Gen. Genet. 260:1998;218-225.
8. Dunaevsky Y.E., Gruban T.N., Beliokova G.A., Belozersky M.A. Enzymes secreted by filamentous fungi: regulation and purification of an extracellular protease of Trichoderma harzianum. Biochemistry (Mosc.). 65:2000;723-727.
9. Dunne C., Moenne-Loccoz Y., de Bruijn F., O'Gara F. Overproduction of an inducible extracellular serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81. Microbiology. 146:2000;2069-2078.
10. Flores A., Chet I., Herrera-Estrella A. Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase-encoding gene prb1. Curr. Genet. 31:1997;30-37.
11. Geremia R.A., Goldman G.H., Jacobs D., Ardiles W., Vila S.B., Van Montagu M., Herrera-Estrella A. Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum. Mol. Microbiol. 8:1993;603-613.
12. Goller S.P., Schoisswohl D., Baron M., Parriche M., Kubicek C.P. Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei. Appl. Environ. Microbiol. 64:1998;3202-3208.
13. Howell C.R., Hanson L.E., Stipanovic R.D., Puckhaber L.S. Induction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani by seed treatments with Trichoderma virens. Phytopathology. 90:2000;248-252.
14. Howell C.R., Stipanovic R.D., Lumsden R.D. Antibiotic production by strains of Gliocladium virens. Biocontrol Sci. Tech. 3:1993;435-441.
15. Kolattukudy P.E., Lee J.D., Rogers L.M., Zimmerman P., Ceselski S., Fox B., Stein B., Copelan E.A. Evidence for possible involvement of an elastolytic serine protease in aspergillosis. Infect. Immun. 61:1993;2357-2368.
16. Marchler G., Schuller C., Adam G., Ruis H. A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 12:1993;1997-2003.
17. Markaryan A., Lee J.D., Sirakova T.D., Kolattukudy P.E. Specific inhibition of mature fungal serine proteinases and metalloproteinases by their propeptides. J. Bacteriol. 178:1996;2211-2215.
18. Messenguy F., Vierendeels F., Scherens B., Dubois E. In Saccharomyces cerevisiae, expression of arginine catabolic genes CAR1 and CAR2 in response to exogenous nitrogen availability is mediated by the Ume6 (CargRI)-Sin3 (CargRII)-Rpd3 (CargRIII) complex. J. Bacteriol. 182:2000;3158-3164.
19. Nelson N.J. Colorimetric analysis of sugars. Methods Enzymol. 3:1957;85-86.
20. Olmedo-Monfil V., Mendoza-Mendoza A., Gómez I., Cortés C., Herrera-Estrella A. Multiple environmental signals determine the transcriptional activation of the mycoparasitism related gene prb1 in Trichoderma atroviride. Mol. Genet. Genomics. 267:2002;703-712.
21. Quandt K., Frech K., Karas H., Wingender E., Werner T. MatInd and MatInspector - new fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res. 23:1995;4878-4884.
22. Ravagnani A., Gorfinkiel L., Langdon T., Diallinas G., Adjadj E., Demais S., Gorton D., Arst H.N. Jr., Scazzocchio C. Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA. EMBO J. 16:1997;3974-3986.
23. Reichard U., Cole G.T., Hill T.W., Ruchel R., Monod M. Molecular characterization and influence on fungal development of ALP2, a novel serine proteinase from Aspergillus fumigatus. Int. J. Med. Microbiol. 290:2000;549-558.
24. Ren Y., West C.A. Elicitation of diterpene biosynthesis in rice (Oryzae sativa L.) by chitin. Plant Physiol. 99:1992;1169-1178.
25. Ren Y., Wee K.E., Chang F.N. Deficiency of current methods in assaying endochitinase activity. Biochem. Biophys. Res. Commun. 268:2000;302-305.
26. Rose, T., Rose, J.M., Di Cera, E., 1999. Serine Protease Web Site. www.biochem.wustl.edu/~protease.
27. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. second ed. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
28. Sophianopoulou V., Suarez T., Diallinas G., Scazzocchio C. Operator derepressed mutations in the proline utilization gene cluster of Aspergillus nidulans. Mol. Gen. Genet. 236:1993;209-213.
29. Sreedhar L., Kobayashi D.Y., Bunting T.E., Hillman B.I., Belanger F.C. Fungal proteinase expression in the interaction of the plant pathogen Magnaporthe poae with its host. Gene. 235:1999;121-129.
30. St Leger R.J., Joshi L., Bidochka M.J., Roberts D.W. Insecticidal properties of a genetically engineered entomopathogenic fungus constitutively expressing a toxic protease. Proc. Natl. Acad. Sci. USA. 93:1996;6349-6354.
31. St Leger R.J., Joshi L., Roberts D. Ambient pH is a major determinant in the expression of cuticle-degrading enzymes and hydrophobin by Metarhizium anisopliae. Appl. Environ. Microbiol. 64:1998;709-713.
32. Tilburn J., Sarkar S., Widdick D.A., Espeso E.A., Orejas M., Mungroo J., Penalva M.A., Arst H.N. Jr. The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J. 14:1995;779-790.
33. Vogel H.J. A convenient growth medium for Neurospora (Medium N). Microbiol. Genet. Bull. 13:1956;42-43.
34. Wiest A., Grzegorski D., Xu B.W., Goulard C., Rebuffat S., Ebbole D.J., Bodo B., Kenerley C.M. Identification of peptaibols from Trichoderma virens and cloning of a peptaibol synthetase. J. Biol. Chem. 277:2002;20862-20868.