Cloning of and genetic variation in protease VCP1 from the nematophagous fungus Pochonia chlamydosporia

Mycological Research

Volumn 107, Issue 1, 2003, Pages 38-46

  Morton, C Oliver ^a,b   Hirsch, Penny R ^a   Peberdy, John P ^b   Kerry, Brian R ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BIOCONTROL AGENT; FUNGUS; GENE; GENETIC VARIATION; NEMATODE; POLYMORPHISM;

FUNGI; METARHIZIUM; METARHIZIUM ANISOPLIAE; MYXOGASTRIA; NEMATODA; POCHONIA; POCHONIA CHLAMYDOSPORIA;

EID: 0037269814     PISSN: 09537562     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0953756202007050     Document Type: Article

References (43)

1. Åhman, J., Ek, B., Rask, L. & Tunlid, A. (1996) Sequence analysis and regulation of a gene encoding a cuticle-degrading serine protease from the nematophagous fungus Arthrobotrys oligospora. Microbiology 142: 1605-1616.
2. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25: 3389-3402.
3. Arora, D. K., Hirsch, P. R. & Kerry, B. R. (1996) PCR-based molecular discrimination of Verticillum chlamydosporium isolates. Mycological Research 100: 801-809.
4. Bidochka, M. J. & Khachatourians, G. G. (1990) Identification of Beauveria bassiana extracellular protease as a virulence factor in pathogenicity toward the Migratory Grasshopper, Melanoplus sanguinipes. Journal of Invertebrate Pathology 56: 362-370.
5. Bidochka, M. J. & Khachatourians, G. G. (1994) Basic proteases of entomopathogenic fungi differ in their adsorption properties to insect cuticle. Journal of Invertebrate Pathology 64: 26-32.
6. Bidochka, M. J., Burke, S. & Ng, L. (1999) Extracellular hydrolytic enzymes in the fungal genus Verticillium: adaptations for pathogenesis. Canadian Journal of Microbiol 45: 856-864.
7. Bird, A. F. & McClure, M. A. (1976) The tylenchid (nematode) egg shell: structure, composition and permeability. Parasitology 72: 19-28.
8. Butt, T. M., Segers, R., Leal, S. C. & Kerry, B. R. (1998) Variation in the subtilisins of fungal pathogens of insects and nematodes. In Molecular Variability of Fungal Pathogens (P. D. Bridge, Y. Couteaudier & J. M. Clarkson, eds): 149-169. CAB International, Wallingford.
9. Clarkson, J. M. & Charnley, A. K. (1996) New insights into the mechanisms of fungal pathogenesis of insects. Trends in Microbiology 4: 197-203.
10. Dhawale, S. S. & Lane, A. C. (1993) Compilation of sequence-specific DNA-binding proteins implicated in transcriptional control in fungi. Nucleic Acids Research 21: 5537-5546.
11. Felsenstein, J. (1989) PHYLIP-Phylogeny Inference Package (Version 3.2). Cladistics 5: 164-166.
12. Frosco, M., Chase, T., jr & MacMillan, J. D. (1992) Purification and properties of the elastase from Aspergillus fumigatus. Infection and Immunity 60: 728-734.
13. Gams, W. & Zare, R. (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 72: 329-337.
14. Gunkel, F. A. & Gassen, H. G. (1989) Proteinase K from Tritirachium album Limber. Characterisation of the chromosomal gene and expression of the cDNA in Escherichia coli. European Journal of Biochemistry 179: 185-194.
15. Isogai, T., Fukagawa, M., Kojo, H., Kohsaka, M., Aoki, H. & Imanaka, H. (1991) Cloning and nucleotide sequences of the complementary and genomic DNAs for the alkaline protease from Acremonium chrysogenum. Agricultural and Biological Chemistry 55: 471-477.
16. Joshi, L., St Leger, R. J. & Bidochka, M. J. (1995) Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana. FEMS Microbiology Letters 125: 211-217.
17. Kerry, B. R. (2000) Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Annual Review of Phytopathology 38: 423-441.
18. Kerry, B. R. & Crump, D. H. (1977) Observations on fungal parasites of females and eggs of the cereal cyst-nematode, Heterodera avenae, and other cyst nematodes. Nematologica 23: 193-201.
19. Kerry, B. R., Crump, D. H. & Mullen, L. A. (1982) Natural control of the cereal cyst-nematode, Heterodera avenae Woll., by soil fungi at three sites. Crop Protection 1: 99-109.
20. Lopez-Llorca, L.V. & Robertson, W.M. (1992) Immunocytochemical localization of a 32-kDa protease from the nematophagous fungus Verticillium suchlasporium in infected nematode eggs. Experimental Mycology 16: 261-267.
21. Morgan-Jones, G., Godoy, G. & Rodriguez-Kabana, R. (1981) Verticillium chlamydosporium, fungal parasite of Meloidogyne arenaria females. Nematropica 11: 115-120.
22. Morita, S., Kuriyama, M., Kazutaka, M. & Kazuaki, K. (1994) Cloning and nucleotide sequence of the alkaline protease gene from Fusarium sp. S-19-5 and expression in Saccharomyces cerevisiae. Bioscience, Biotechnology and Biochemistry 58: 621-626.
23. Murakami, K., Ishida, Y., Masaki, A., Tatsumi, H., Murakami, S., Nakano, E., Motai, H., Kawabe, H. & Arimura, H. (1991) Isolation and characterisation of the alkaline protease gene of Aspergillus oryzae. Agricultural and Biological Chemistry 55: 2807-2811.
24. Page, R. D. M. (1996) TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357-358.
25. Perona, J. J. & Craik, C. S. (1995) Structural basis of substrate specificity in the serine proteases. Protein Science 4: 337-360.
26. Screen, S., Bailey, A., Charnley, A. K., Cooper, R. M. & Clarkson, J. M. (1997) Carbon regulation of the cuticle-degrading enzyme PR1 from Metarhizium anisopliae may involve a trans-acting DNA-binding protein CRR1, a functional equivalent of the Aspergillus nidulans CREA protein. Current Genetics 31: 511-518.
27. Segers, R., Butt, T. M., Kerry, B. R. & Peberdy, J. F. (1994) The nematophagous fungus Verticillium chlamydosporium produces a chymoelastase-like protease which hydrolyses host nematode proteins in situ. Microbiology 140: 2715-2723.
28. Segers, R., Butt, T. M., Keen, J. N., Kerry, B. R. & Peberdy, J. F. (1995) The subtilisins of the invertebrate mycopathogens Verticillium chlamydosporium and Metarhizium anisopliae are serologically and functionally related. FEMS Microbiology Letters 126: 227-231.
29. Segers, R. (1996a) The nematophagous fungus Verticillium chlamydosporium, aspects of pathogenicity. PhD thesis, University of Nottingham.
30. Segers, R., Butt, T. M., Kerry, B. R., Beckett, A. & Peberdy, J. F. (1996b) The role of the proteinase VCP1 produced by the nematophagous Verticillium chlamydosporium in the infection process of nematode eggs. Mycological Research 100: 421-428.
31. Segers, R., Butt, T. M., Carder, J. H., Keen, J. F., Kerry, B. R. & Peberdy, J. F. (1999) The subtilisins of fungal pathogens of insects, nematodes and plants: distribution and variation. Mycological Research 103: 395-402.
32. Siezen, R. J., Devos, W. M., Leunissen, J. A. M. & Dijkstra, B. W. (1991) Homology modeling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases. Protein Engineering 4: 719-737.
33. Siezen, R. J. & Leunissen, J. A. M. (1997) Subtilases: the superfamily of subtilisin-like serine proteases. Protein Science 6: 501-523.
34. Sørensen, A. B., Duch, M. & Pedersen, F. S. (1993) Isolation of unknown flanking DNA by a simple two-step polymerase chain reaction method. Dynabeads® M-280 Streptavidin (Prod. No. 112.05/06 and 602.10) applications. http://www.dynal.no/.
35. St Leger, R. J., Charnley, A. K. & Cooper, R. M. (1987) Characterization of cuticle-degrading proteases produced by the entomopathogen Metarhizium anisopliae. Archives of Biochemistry and Biophysics 253: 221-232.
36. St Leger, R. J., Frank, D. C., Roberts, D. W. & Staples, R. C. (1992) Molecular cloning and regulatory analysis of the cuticle-degrading-protease structural gene from the entomopathogenic fungus Metarhizium anisopliae. European Journal of Biochemistry 204: 991-1001.
37. St Leger, R. J. (1995) The role of cuticle-degrading proteases in fungal pathogenesis of insects. Canadian Journal of Botany 73 (Suppl.): S1119-S1125.
38. St Leger, R. J., Joshi, L., Bidochka, M. J. & Roberts, D. W. (1996) Construction of an improved mycoinsecticide overexpressing a toxic protease. Proceedings of the National Academy of Sciences, USA 93: 6349-6354.
39. Staden, R. (1996) The Staden sequence analysis package. Molecular Biotechnology 5: 233-241.
40. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876-4882.
41. Tunlid, A., Rosen, S., Ek, B. & Rask, R. (1994) Purification and characterisation of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys oligospora. Microbiology 140: 1687-1695.
42. Wells, J. A. & Estell, D. A. (1988) Subtilisin - an enzyme designed to be engineered. Trends in Biochemical Sciences 13: 291-297.
43. Williamson, M. S., Martinez-Torres, D., Hick, C. A. & Devonshire, A. L. (1996) Identification of mutations in the housefly para-type sodium channel gene associated with knockdown resistance (kdr) to pyrethroid insecticides. Molecular and General Genetics 252: 51-60.